BBa_K1884006 1 BBa_K1884006 Ferredoxin-NADP(+)Reductase(FNR) 2016-10-08T11:00:00Z 2016-10-17T07:43:45Z The DNA sequence of FNR is designed by ourselves Under anaerobic conditions, fumarate or nitrate, among others, can replace O2 as the terminal electron acceptor. Optimal switching from one respiratory pathway to another is thus a key requirement for this flexibility. In E. coli, the fumarate and nitrate reduction (FNR) transcriptional regulator is responsible for sensing environmental levels of O2 and controlling the switch to anaerobic respiration false false _2349_ 29275 29275 9 false false YongJing Ping BBa_K509003 1 BBa_K509003 PSAD Terminator 2011-09-18T11:00:00Z 2015-05-08T01:12:30Z plasmid PSAD Terminator false false _672_ 0 8421 9 Not in stock false to edit false Ben Jevans BBa_K1884009 1 BBa_K1884009 PSAD Promoter+GalAD-CRY2+UAS+FNR(fumarate and nitrate reduction)+PSAD Terminator 2016-10-10T11:00:00Z 2016-10-19T05:48:42Z false false _2349_ 29275 29275 9 false false Changxing Hu component2492806 1 BBa_K1547005 component2492809 1 BBa_K1884004 component2492810 1 BBa_K1884006 component2492811 1 BBa_K509003 component2492807 1 BBa_K1884002 annotation2492807 1 BBa_K1884002 range2492807 1 901 3006 annotation2492806 1 BBa_K1547005 range2492806 1 1 894 annotation2492809 1 BBa_K1884004 range2492809 1 3015 3193 annotation2492810 1 BBa_K1884006 range2492810 1 3202 3342 annotation2492811 1 BBa_K509003 range2492811 1 3351 3883 BBa_K1884002 1 AD-CRY2 VP16AD-CRY2 2016-10-06T11:00:00Z 2016-10-13T08:55:18Z Part cloned from pRMH120 of Addgene Cryptochrome 2 (CRY2) is a blue light stimulated photoreceptor, when exposed to blue light, it would interact with CIB1. This part is a Gal4 DNA activating domain fused to N terminus of CRY2. false false _2349_ 29275 30234 9 Not in stock false One powerful application of protein-interaction dimerizers(CRY2-CIB1) is to allow inducible control of a 'split' protein, in which two inactive fragments are brought together to reconstitute a functional protein activity. In principle, using this approach with optically controlled dimerizers would confer light-dependent activity to a diverse range of target proteins. We used the CRY2-CIB1 modules to reconstitute split versions of Gal4 transcription factor proteins. Based on the transient nature of the CRY-CIB interaction,the light pulses would allow precise, dose-dependent control of protein activity. We expressed in yeast the split Gal4 partners Gal4BD- CIB1 and Gal4AD-CRY2 together with upstream activating sequence under control of a galactose-inducible promoter. We incubated the yeast in the dark or subjected them to up to twenty blue-light pulses over 4 h. Immunoblot analysis of Snl1 revealed a strong dose dependence of protein expression in response to light. false Chengrong Xie BBa_K1547005 1 BBa_K1547005 PSAD Promoter 2014-10-07T11:00:00Z 2015-05-08T01:10:53Z Chlamydomonas reinhardtii nucleus This is a plant promoter from the green algae Chlamydomonas reinhardtii. false false _1931_ 0 22390 9 It's complicated false PSAD was amplified using PCR on a plasmid containing the promoter supplied by chlamycollection.org false victor yuan BBa_K1884004 1 Gal4-UAS Upstream activating sequence 2016-10-06T11:00:00Z 2016-10-15T07:42:46Z PhyB (photochrome B) PhyB is red/far-red photoreceptor involved in the regulation of de-etiolation. It is involved in the light-promotion of seed germination and in the shade avoidance response. false false _2349_ 29275 30234 9 No part sequence false PhyB functions as a transcriptional activator, and can be functionally and mechanistically separated from its role in repression of PIF3 mediated processes. false Chengrong Xie annotation2489054 1 UAS range2489054 1 1 179 BBa_K1884002_sequence 1 atgggccccaagaagaagcgcaaggtggccccccccaccgacgtgagcctgggcgacgagctgcacctggacggcgaggacgtggcgatggcccacgccgacgccctggacgacttcgacctggacatgctgggcgacggcgacagccccggccccggcttcaccccccacgacagcgccccctacggcgccctggacatggccgacttcgagttcgagcagatgttcaccgacgccctgggcatcgacgagtacggcggccatatgatgaagatggacaaaaagactatagtttggtttagaagagacctaaggattgaggataatcctgcattagcagcagctgctcacgaaggatctgtttttcctgtcttcatttggtgtcctgaagaagaaggacagttttatcctggaagagcttcaagatggtggatgaaacaatcacttgctcacttatctcaatccttgaaggctcttggatctgacctcactttaatcaaaacccacaacacgatttcagcgatcttggattgtatccgcgttaccggtgctacaaaagtcgtctttaaccacctctatgatcctgtttcgttagttcgggaccataccgtaaaggagaagctggtggaacgtgggatctctgtgcaaagctacaatggagatctattgtatgaaccgtgggagatatactgcgaaaagggcaaaccttttacgagtttcaattcttactggaagaaatgcttagatatgtcgattgaatccgttatgcttcctcctccttggcggttgatgccaataactgcggcggctgaagcgatttgggcgtgttcgattgaagaactagggctggagaatgaggccgagaaaccgagcaatgcgttgttaactagagcttggtctccaggatggagcaatgctgataagttactaaatgagttcatcgagaagcagttgatagattatgcaaagaacagcaagaaagttgttgggaactctacttcactactttctccgtatctccatttcggggaaataagcgtcagacacgttttccagtgtgcccggatgaaacaaattatatgggcaagagataagaacagtgaaggagaagaaagtgcagatctttttcttaggggaatcggtttaagagagtattctcggtatatatgtttcaacttcccgtttactcacgagcaatcgttgttgagtcatcttcggtttttcccttgggatgctgatgttgataagttcaaggcctggagacaaggcaggaccggttatccgttggtggatgccggaatgagagagctttgggctaccggatggatgcataacagaataagagtgattgtttcaagctttgctgtgaagtttcttctccttccatggaaatggggaatgaagtatttctgggatacacttttggatgctgatttggaatgtgacatccttggctggcagtatatctctgggagtatccccgatggccacgagcttgatcgcttggacaatcccgcgttacaaggcgccaaatatgacccagaaggtgagtacataaggcaatggcttcccgagcttgcgagattgccaactgaatggatccatcatccatgggacgctcctttaaccgtactcaaagcttctggtgtggaactcggaacaaactatgcgaaacccattgtagacatcgacacagctcgtgagctactagctaaagctatttcaagaacccgtgaagcacagatcatgatcggagcagcacctgatgagattgtagcagatagcttcgaggccttaggggctaataccattaaagaacctggtctttgcccatctgtgtcttctaatgaccaacaagtaccttcggctgttcgttacaacgggtcaaagagagtgaaacctgaggaagaagaagagagagacatgaagaaatctaggggattcgatgaaagggagttgttttcgactgctgaatcttcttcttcttcgagtgtgtttttcgtttcgcagtcttgctcgttggcatcagaagggaagaatctggaaggtattcaagattcatctgatcagattactacaagtttgggaaaaaatggttgcaaatga BBa_K1547005_sequence 1 taattcatcaaattataactatatagaccctaatttcatacacacacctgcccgtctgcctgacaggaagtgaacgcatgtcgagggaggcctcaccaatcgtcacacgagccctcgtcagaaacacgtctccgccacgctctccctctcacggccgaccccgcagcccttttgccctttcctaggccaccgacaggacccaggcgctctcagcatgcctcaacaacccgtactcgtgccagcggtgcccttgtgctggtgatcgcttggaagcgcatgcgaagacgaaggggcggagcaggcggcctggctgttcgaagggctcgccgccagttcgggtgcctttctccacgcgcgcctccacacctaccgatgcgtgaaggcaggcaaatgctcatgtttgcccgaactcggagtccttaaaaagccgcttcttgtcgtcgttccgagacatgttagcagatcgcagtgccacctttcctgacgcgctcggccccatattcggacgcaattgtcatttgtagcacaattggagcaaatctggcgaggcagtaggcttttaagttgcaaggcgagagagcaaagtgggacgcggcgtgattattggtatttacgcgacggcccggcgcgttagcggcccttcccccaggccagggacgattatgtatcaatattgttgcgttcgggcactcgtgcgagggctcctgcgggctggggagggggatctgggaattggaggtacgaccgagatggcttgctcggggggaggtttcctcgccgagcaagccagggttaggtgttgcgctcttgactcgttgtgcattctaggaccccactgctactcacaacaagccctaactgtgccaaattatcttccaaactcctaatccaatt BBa_K1884004_sequence 1 cggagtactgtcctccgagcggagtactgtcctccgactcgagcggagtactgtcctccgatcggagtactgtcctccgcgaatcccggagtactgtcctccgaagacgctagcggggggctataaaagggggtgggggcgttcgtcctcactctattagtattttaaatacctagata BBa_K1884009_sequence 1 taattcatcaaattataactatatagaccctaatttcatacacacacctgcccgtctgcctgacaggaagtgaacgcatgtcgagggaggcctcaccaatcgtcacacgagccctcgtcagaaacacgtctccgccacgctctccctctcacggccgaccccgcagcccttttgccctttcctaggccaccgacaggacccaggcgctctcagcatgcctcaacaacccgtactcgtgccagcggtgcccttgtgctggtgatcgcttggaagcgcatgcgaagacgaaggggcggagcaggcggcctggctgttcgaagggctcgccgccagttcgggtgcctttctccacgcgcgcctccacacctaccgatgcgtgaaggcaggcaaatgctcatgtttgcccgaactcggagtccttaaaaagccgcttcttgtcgtcgttccgagacatgttagcagatcgcagtgccacctttcctgacgcgctcggccccatattcggacgcaattgtcatttgtagcacaattggagcaaatctggcgaggcagtaggcttttaagttgcaaggcgagagagcaaagtgggacgcggcgtgattattggtatttacgcgacggcccggcgcgttagcggcccttcccccaggccagggacgattatgtatcaatattgttgcgttcgggcactcgtgcgagggctcctgcgggctggggagggggatctgggaattggaggtacgaccgagatggcttgctcggggggaggtttcctcgccgagcaagccagggttaggtgttgcgctcttgactcgttgtgcattctaggaccccactgctactcacaacaagccctaactgtgccaaattatcttccaaactcctaatccaatttactagatgggccccaagaagaagcgcaaggtggccccccccaccgacgtgagcctgggcgacgagctgcacctggacggcgaggacgtggcgatggcccacgccgacgccctggacgacttcgacctggacatgctgggcgacggcgacagccccggccccggcttcaccccccacgacagcgccccctacggcgccctggacatggccgacttcgagttcgagcagatgttcaccgacgccctgggcatcgacgagtacggcggccatatgatgaagatggacaaaaagactatagtttggtttagaagagacctaaggattgaggataatcctgcattagcagcagctgctcacgaaggatctgtttttcctgtcttcatttggtgtcctgaagaagaaggacagttttatcctggaagagcttcaagatggtggatgaaacaatcacttgctcacttatctcaatccttgaaggctcttggatctgacctcactttaatcaaaacccacaacacgatttcagcgatcttggattgtatccgcgttaccggtgctacaaaagtcgtctttaaccacctctatgatcctgtttcgttagttcgggaccataccgtaaaggagaagctggtggaacgtgggatctctgtgcaaagctacaatggagatctattgtatgaaccgtgggagatatactgcgaaaagggcaaaccttttacgagtttcaattcttactggaagaaatgcttagatatgtcgattgaatccgttatgcttcctcctccttggcggttgatgccaataactgcggcggctgaagcgatttgggcgtgttcgattgaagaactagggctggagaatgaggccgagaaaccgagcaatgcgttgttaactagagcttggtctccaggatggagcaatgctgataagttactaaatgagttcatcgagaagcagttgatagattatgcaaagaacagcaagaaagttgttgggaactctacttcactactttctccgtatctccatttcggggaaataagcgtcagacacgttttccagtgtgcccggatgaaacaaattatatgggcaagagataagaacagtgaaggagaagaaagtgcagatctttttcttaggggaatcggtttaagagagtattctcggtatatatgtttcaacttcccgtttactcacgagcaatcgttgttgagtcatcttcggtttttcccttgggatgctgatgttgataagttcaaggcctggagacaaggcaggaccggttatccgttggtggatgccggaatgagagagctttgggctaccggatggatgcataacagaataagagtgattgtttcaagctttgctgtgaagtttcttctccttccatggaaatggggaatgaagtatttctgggatacacttttggatgctgatttggaatgtgacatccttggctggcagtatatctctgggagtatccccgatggccacgagcttgatcgcttggacaatcccgcgttacaaggcgccaaatatgacccagaaggtgagtacataaggcaatggcttcccgagcttgcgagattgccaactgaatggatccatcatccatgggacgctcctttaaccgtactcaaagcttctggtgtggaactcggaacaaactatgcgaaacccattgtagacatcgacacagctcgtgagctactagctaaagctatttcaagaacccgtgaagcacagatcatgatcggagcagcacctgatgagattgtagcagatagcttcgaggccttaggggctaataccattaaagaacctggtctttgcccatctgtgtcttctaatgaccaacaagtaccttcggctgttcgttacaacgggtcaaagagagtgaaacctgaggaagaagaagagagagacatgaagaaatctaggggattcgatgaaagggagttgttttcgactgctgaatcttcttcttcttcgagtgtgtttttcgtttcgcagtcttgctcgttggcatcagaagggaagaatctggaaggtattcaagattcatctgatcagattactacaagtttgggaaaaaatggttgcaaatgatactagagcggagtactgtcctccgagcggagtactgtcctccgactcgagcggagtactgtcctccgatcggagtactgtcctccgcgaatcccggagtactgtcctccgaagacgctagcggggggctataaaagggggtgggggcgttcgtcctcactctattagtattttaaatacctagatatactagaggcggggccctgacaccactgcggccgccacctgccacatcatcatacgccgcgcctggacccgagggaggacccctcgggacccggtacgtcgtatgatgatgtggcaagtcttggtcgcggtggggtcaggtccttccgctactagagttctggcagcagctggaccgcctgtaccatggagaagagctttacttgccgggatggccgatttcgctgattgatacgggatcggagctcggaggctttcgcgctaggggctaggcgaagggcagtggtgaccagggtcggtgtggggtcggcccacggtcaattagccacaggaggatcagggggaggtaggcacgtcgacttggtttgcgaccccgcagttttggcggacgtgctgttgtagatgttagcgtgtgcgtgagccagtggccaacgtgccacacccattgagaagaccaaccaacttactggcaatatctgccaatgccatactgcatgtaatggccaggccatgtgagagtttgccgtgcctgcgcgcgccccgggggcgcagtttagctgaccagccgtgggatgatgcacgcatttgcaaggacagggtaatcacagcagcaacatggtgggcttaggacagctgtgggtcagtggacggacggcaggggagggacggcgcagctcgggagacaggggga BBa_K1884006_sequence 1 gcggggccctgacaccactgcggccgccacctgccacatcatcatacgccgcgcctggacccgagggaggacccctcgggacccggtacgtcgtatgatgatgtggcaagtcttggtcgcggtggggtcaggtccttccgc BBa_K509003_sequence 1 ttctggcagcagctggaccgcctgtaccatggagaagagctttacttgccgggatggccgatttcgctgattgatacgggatcggagctcggaggctttcgcgctaggggctaggcgaagggcagtggtgaccagggtcggtgtggggtcggcccacggtcaattagccacaggaggatcagggggaggtaggcacgtcgacttggtttgcgaccccgcagttttggcggacgtgctgttgtagatgttagcgtgtgcgtgagccagtggccaacgtgccacacccattgagaagaccaaccaacttactggcaatatctgccaatgccatactgcatgtaatggccaggccatgtgagagtttgccgtgcctgcgcgcgccccgggggcgcagtttagctgaccagccgtgggatgatgcacgcatttgcaaggacagggtaatcacagcagcaacatggtgggcttaggacagctgtgggtcagtggacggacggcaggggagggacggcgcagctcgggagacaggggga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z