##gff-version 3
##sequence-region BBa_K1885000 1 1155
BBa_K1885000	.	engineered_region	44	1112	.	+	0	ID=BBa_J04450;Name=BBa_J04450
BBa_K1885000	.	terminator	1072	1112	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J04450
BBa_K1885000	.	polyA_site	1099	1112	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1885000	.	stem_loop	1079	1098	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1885000	.	engineered_region	1072	1112	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1885000	.	stop_codon	1105	1105	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1885000	.	ribosome_entry_site	252	263	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J04450
BBa_K1885000	.	conserved_region	256	259	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1885000	.	CDS	270	950	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_J04450
BBa_K1885000	.	CDS	270	944	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K1885000	.	engineered_tag	951	975	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K1885000	.	terminator	984	1063	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J04450
BBa_K1885000	.	stem_loop	995	1038	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1885000	.	engineered_region	984	1063	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1885000	.	promoter	44	243	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_J04450
BBa_K1885000	.	CDS	44	131	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K1885000	.	engineered_region	44	243	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K1885000	.	start_codon	216	216	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K1885000	.	promoter	180	185	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K1885000	.	non_covalent_binding_site	132	169	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K1885000	.	promoter	204	209	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K1885000	.	non_covalent_binding_site	209	243	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K1885000	.	promoter	1	35	.	+	0	ID=BBa_J23100;Name=BBa_J23100
BBa_K1885000	.	promoter	1121	1155	.	+	0	ID=BBa_J23100;Name=BBa_J23100
>BBa_K1885000
ttgacggctagctcagtcctaggtacagtgctagctactagagcaatacgcaaaccgcctctccccgcgcgttggccga
ttcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagct
cactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaattt
cacacatactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagt
tcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccag
accgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggtt
ccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacg
tgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaa
gttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccg
aacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacga
cgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggac
atcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaat
aacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcc
tttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcg
tttatatactagagttgacggctagctcagtcctaggtacagtgctagc