BBa_J18921
1
3xGS
6aa [GS]x linker
2010-01-26T12:00:00Z
2015-08-31T04:08:36Z
gene synthesis
Highly flexible 6 amino acid linker. Translates to gsgsgs. Codon-optimize for E. coli, yeast, mammalian.
false
false
_165_
0
2175
165
It's complicated
false
* gene synthesis
* codon optimized for E. coli
false
Raik Gruenberg
BBa_K1885001
1
osmY-PETas
Novel fusion osmY-PETase, A1.00
2016-10-13T11:00:00Z
2016-10-29T11:16:17Z
The pSB1C3 backbone, the Anderson promoter, and serine-glycine linker peptide parts came from the iGEM registry. The N-terminal osmY sequence came from the Escherischia Coli genome, found on NCBI, and the PETase protein came from the Ideonella sakaiensis genome, found on NCBI and annotated as ???lipase."
PETase is a novel enzyme for the degradation of polyethylene terephthalate (PET) plastic. It was discovered in Ideonella sakaiensis, a bacteria species found by screening PET-exposed microbe communities in Japan. It functions as the first step in the degradation of PET; PETase catalyzes the hydrolysis of PET into terephthalate and ethylene glycol monomers.
The N-terminal osmY protein and its signal peptide fused to PETase, with serine-glycine peptide repeats intervening, serves to secrete the PETase out of the cell so that it can act on PET in the cell's local environment.
The Anderson promoter with a relative strength of 1.00 that precedes the fusion protein serves to constitutively express the fusion PETase, enhancing the degradation of environmental PET by the presence of more fusion PETase.
This part offers a more efficient initial step for teams interested in the bioremediation of PET, because the first step involved in PET bioremediation is hydrolysis into products that can then be utilized by a cell. Teams can then metabolically engineer bacteria of interest to metabolize ethylene glycol and terephthalate, but initially, these monomers must be produced by hydrolysis. Thus, PET bioremediation depends on the production of metabolizable monomers, and this part offers more efficient production of such monomers.
This part utilizes the pSB1C3 backbone and confers resistance to chloramphenicol.
true
false
_2350_
29517
29517
9
false
Designing this part required considering constitutive expression and promoter strength, secretion of the PETase, and maintaining an active PETase conformation. Respectively, the Anderson promoter, osmY, and serine-glycine linker peptide address the aforementioned considerations.
false
Kien Patrick Malarney
component2530879
1
BBa_J23100
component2530880
1
BBa_E0010
component2530878
1
BBa_J18921
annotation2530879
1
BBa_J23100
range2530879
1
27
61
annotation2530880
1
BBa_E0010
range2530880
1
68
748
annotation2530878
1
BBa_J18921
range2530878
1
1
18
BBa_E0010
1
dsRed
dsRed (LVA-)
2004-07-06T11:00:00Z
2015-08-31T04:07:25Z
-- No description --
false
false
_11_1_
0
60
7
Not in stock
false
false
cconboy
BBa_J23100
1
BBa_J23100
constitutive promoter family member
2006-08-03T11:00:00Z
2015-08-31T04:08:40Z
Isolated from library of promoters
Released HQ 2013
Replace later
false
true
_52_
0
483
95
In stock
true
N/A
true
John Anderson
BBa_J23100_sequence
1
ttgacggctagctcagtcctaggtacagtgctagc
BBa_K1885001_sequence
1
ggtagcggcagcggtagctactagagttgacggctagctcagtcctaggtacagtgctagctactagatggcctcctccgaggacgtcatcaaggagttcatgcgcttcaaggtgcgcatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggcggccccctgcccttcgcctgggacatcctgtccccccagttccagtacggctccaaggtgtacgtgaagcaccccgccgacatccccgactacaagaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccctgcaagacggctccttcatctacaaggtgaagttcatcggcgtgaacttcccctccgacggccccgtaatgcagaagaagactatgggctgggaggcctccaccgagcgcctgtacccccgcgacggcgtgctgaagggcgagatccacaaggccctgaagctgaaggacggcggccactacctggtggagttcaagtccatctacatggccaagaagcccgtgcagctgcccggctactactacgtggactccaagctggacatcacctcccacaacgaggactacaccatcgtggagcagtacgagcgcgccgagggccgccaccacctgttcctgtaataa
BBa_J18921_sequence
1
ggtagcggcagcggtagc
BBa_E0010_sequence
1
atggcctcctccgaggacgtcatcaaggagttcatgcgcttcaaggtgcgcatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggcggccccctgcccttcgcctgggacatcctgtccccccagttccagtacggctccaaggtgtacgtgaagcaccccgccgacatccccgactacaagaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccctgcaagacggctccttcatctacaaggtgaagttcatcggcgtgaacttcccctccgacggccccgtaatgcagaagaagactatgggctgggaggcctccaccgagcgcctgtacccccgcgacggcgtgctgaagggcgagatccacaaggccctgaagctgaaggacggcggccactacctggtggagttcaagtccatctacatggccaagaagcccgtgcagctgcccggctactactacgtggactccaagctggacatcacctcccacaacgaggactacaccatcgtggagcagtacgagcgcgccgagggccgccaccacctgttcctgtaataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z