BBa_K1885123 1 BBa_K1885123 Novel osmY-PETase fusion protein, A0.33 2016-10-28T11:00:00Z 2016-10-29T11:20:21Z The osmY and PETase sequences were drawn from genome sequences of escherichia coli and ideonella sakanesis, respectively. PETase is a novel enzyme for the degradation of polyethylene terephthalate (PET) plastic. It was discovered in Ideonella sakaiensis, a bacteria species found by screening PET-exposed microbe communities in Japan. It functions as the first step in the degradation of PET; PETase catalyzes the hydrolysis of PET into terephthalate and ethylene glycol monomers. The N-terminal osmY protein and its signal peptide fused to PETase, with serine-glycine peptide repeats intervening, serves to secrete the PETase out of the cell so that it can act on PET in the cell's local environment. The Anderson promoter with a relative strength of 0.33 that precedes the fusion protein serves to constitutively express the fusion PETase, enhancing the degradation of environmental PET by the presence of more fusion PETase by constitutive expression. This part offers a more efficient initial step for teams interested in the bioremediation of PET, because the first step involved in PET bioremediation is hydrolysis into products that can then be utilized by a cell. Teams can then metabolically engineer bacteria of interest to metabolize ethylene glycol and terephthalate, but initially, these monomers must be produced by hydrolysis. Thus, PET bioremediation depends on the production of metabolizable monomers, and this part offers more efficient production of such monomers. false false _2350_ 29517 29517 9 false We added the glycine-serine linker because we considered the c-terminal osmY domains and n-terminal PETase domains interfering with one another's folding. false Kien Patrick Malarney component2530884 1 BBa_K1885120 component2530882 1 BBa_K1885007 component2530881 1 BBa_J23110 component2530883 1 BBa_J18921 annotation2530884 1 BBa_K1885120 range2530884 1 680 1552 annotation2530881 1 BBa_J23110 range2530881 1 1 35 annotation2530883 1 BBa_J18921 range2530883 1 656 673 annotation2530882 1 BBa_K1885007 range2530882 1 42 647 BBa_J23110 1 BBa_J23110 constitutive promoter family member 2006-08-16T11:00:00Z 2015-08-31T04:08:40Z Later Later false true _52_ 0 483 95 In stock true N/A true John Anderson BBa_J18921 1 3xGS 6aa [GS]x linker 2010-01-26T12:00:00Z 2015-08-31T04:08:36Z gene synthesis Highly flexible 6 amino acid linker. Translates to gsgsgs. Codon-optimize for E. coli, yeast, mammalian. false false _165_ 0 2175 165 It's complicated false * gene synthesis * codon optimized for E. coli false Raik Gruenberg BBa_K1885007 1 BBa_K1885007 osmY 2016-10-28T11:00:00Z 2016-10-29T11:12:20Z The coding region was drawn from the escherichia coli genome sequence. osmY is an osmotically inducible protein found in escherichia coli and is secreted out of the cell. This part codes for osmY. false false _2350_ 29517 29517 9 false The coding region was drawn from the escherichia coli genome sequence. false Kien Patrick Malarney BBa_K1885120 1 BBa_K1885120 PETase from Ideonella Sakainesis 2016-10-28T11:00:00Z 2016-10-29T11:04:38Z This part comes from a shotgun sequence of the Ideonella Sakainesis genome. Isolated from Ideonella Sakarinesis, this is the coding sequence for the enzyme PETase, which catalyzes the hydrolysis of polyethylene terephthalate into ethylene glycol and terephthalic acid. It codes for a PET hydrolase. false false _2350_ 29517 29517 9 false It was taken directly from the genome sequence. false Kien Patrick Malarney BBa_K1885120_sequence 1 atgaactttccccgcgcttcccgcctgatgcaggccgccgttctcggcgggctgatggccgtgtcggccgccgccaccgcccagaccaacccctacgcccgcggcccgaacccgacagccgcctcactcgaagccagcgccggcccgttcaccgtgcgctcgttcaccgtgagccgcccgagcggctacggcgccggcaccgtgtactaccccaccaacgccggcggcaccgtgggcgccatcgccatcgtgccgggctacaccgcgcgccagtcgagcatcaaatggtggggcccgcgcctggcctcgcacggcttcgtggtcatcaccatcgacaccaactccacgctcgaccagccgtccagccgctcgtcgcagcagatggccgcgctgcgccaggtggcctcgctcaacggcaccagcagcagcccgatctacggcaaggtcgacaccgcccgcatgggcgtgatgggctggtcgatgggcggtggcggctcgctgatctcggcggccaacaacccgtcgctgaaagccgcggcgccgcaggccccgtgggacagctcgaccaacttctcgtcggtcaccgtgcccacgctgatcttcgcctgcgagaacgacagcatcgccccggtcaactcgtccgccctgccgatctacgacagcatgtcgcgcaatgcgaagcagttcctcgagatcaacggtggctcgcactcctgcgccaacagcggcaacagcaaccaggcgctgatcggcaagaagggcgtggcctggatgaagcgcttcatggacaacgacacgcgctactccaccttcgcctgcgagaacccgaacagcacccgcgtgtcggacttccgcaccgcgaactgcagctga BBa_K1885123_sequence 1 tttacggctagctcagtcctaggtacaatgctagctactagatgactatgacaagactgaagatttcgaaaactctgctggctgtaatgttgacctctgccgtcgcgaccggctctgcctacgcggaaaacaacgcgcagactaccaatgaaagcgcagggcaaaaagtcgatagctctatgaataaagtcggtaatttcatggatgacagcgccatcaccgcgaaagtgaaggcggccctggtggatcatgacaacatcaagagcaccgatatctctgtaaaaaccgatcaaaaagtcgtgaccctgagcggtttcgttgaaagccaggcccaggccgaagaggcagtgaaagtggcgaaaggcgttgaaggggtgacctctgtcagcgacaaactgcacgttcgcgacgctaaagaaggctcggtgaagggctacgcgggtgacaccgccaccaccagtgaaatcaaagccaaactgctggcggacgatatcgtcccttcccgtcatgtgaaagttgaaaccaccgacggcgtggttcagctctccggtaccgtcgattctcaggcacaaagtgaccgtgctgaaagtatcgccaaagcggtagatggtgtgaaaagcgttaaaaatgatctgaaaactaagtaatactagagggtagcggcagcggtagctactagatgaactttccccgcgcttcccgcctgatgcaggccgccgttctcggcgggctgatggccgtgtcggccgccgccaccgcccagaccaacccctacgcccgcggcccgaacccgacagccgcctcactcgaagccagcgccggcccgttcaccgtgcgctcgttcaccgtgagccgcccgagcggctacggcgccggcaccgtgtactaccccaccaacgccggcggcaccgtgggcgccatcgccatcgtgccgggctacaccgcgcgccagtcgagcatcaaatggtggggcccgcgcctggcctcgcacggcttcgtggtcatcaccatcgacaccaactccacgctcgaccagccgtccagccgctcgtcgcagcagatggccgcgctgcgccaggtggcctcgctcaacggcaccagcagcagcccgatctacggcaaggtcgacaccgcccgcatgggcgtgatgggctggtcgatgggcggtggcggctcgctgatctcggcggccaacaacccgtcgctgaaagccgcggcgccgcaggccccgtgggacagctcgaccaacttctcgtcggtcaccgtgcccacgctgatcttcgcctgcgagaacgacagcatcgccccggtcaactcgtccgccctgccgatctacgacagcatgtcgcgcaatgcgaagcagttcctcgagatcaacggtggctcgcactcctgcgccaacagcggcaacagcaaccaggcgctgatcggcaagaagggcgtggcctggatgaagcgcttcatggacaacgacacgcgctactccaccttcgcctgcgagaacccgaacagcacccgcgtgtcggacttccgcaccgcgaactgcagctga BBa_K1885007_sequence 1 atgactatgacaagactgaagatttcgaaaactctgctggctgtaatgttgacctctgccgtcgcgaccggctctgcctacgcggaaaacaacgcgcagactaccaatgaaagcgcagggcaaaaagtcgatagctctatgaataaagtcggtaatttcatggatgacagcgccatcaccgcgaaagtgaaggcggccctggtggatcatgacaacatcaagagcaccgatatctctgtaaaaaccgatcaaaaagtcgtgaccctgagcggtttcgttgaaagccaggcccaggccgaagaggcagtgaaagtggcgaaaggcgttgaaggggtgacctctgtcagcgacaaactgcacgttcgcgacgctaaagaaggctcggtgaagggctacgcgggtgacaccgccaccaccagtgaaatcaaagccaaactgctggcggacgatatcgtcccttcccgtcatgtgaaagttgaaaccaccgacggcgtggttcagctctccggtaccgtcgattctcaggcacaaagtgaccgtgctgaaagtatcgccaaagcggtagatggtgtgaaaagcgttaaaaatgatctgaaaactaagtaa BBa_J18921_sequence 1 ggtagcggcagcggtagc BBa_J23110_sequence 1 tttacggctagctcagtcctaggtacaatgctagc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z