LOCUS BBa_K1886002 4850 bp DNA linear 28-Nov-2024 DEFINITION AHL,DPD double-cycled oscillation ACCESSION BBa_K1886002 VERSION BBa_K1886002.1 FEATURES Location/Qualifiers misc_feature 1..20 /label=LuxR/HSL promoter 115..122 /label=-10 promoter 89..94 /label=-35 misc_feature 128..128 /label=start misc_feature 130..149 /label=Putative LuxR/HSL misc_feature 1..151 /label=BBa_R0063 promoter 1..151 /label=BBa_R0063 misc_feature 164..167 /label=conserved RBS 160..171 /label=BBa_B0034 misc_feature 178..179 /label=prefix misc_feature 351..351 /label=T misc_feature 669..669 /label=A CDS 178..927 /label=luxR misc_feature 178..933 /label=BBa_C0062 misc_feature 934..958 /label=Help:Barcodes CDS 178..933 /label=BBa_C0062 stem_loop 978..1021 /label=stem_loop misc_feature 967..1046 /label=BBa_B0010 terminator 967..1046 /label=BBa_B0010 stem_loop 1062..1081 /label=T7 TE misc_feature 1088..1088 /label=stop polyA_site 1082..1095 /label=polya misc_feature 1055..1095 /label=BBa_B0012 terminator 1055..1095 /label=BBa_B0012 terminator 967..1095 /label=BBa_B0015 misc_feature 1104..1123 /label=LuxR/HSL promoter 1123..1128 /label=-35 promoter 1145..1150 /label=-10 misc_feature 1156..1156 /label=start misc_feature 1104..1158 /label=BBa_R0062 promoter 1104..1158 /label=BBa_R0062 misc_feature 1171..1174 /label=conserved RBS 1167..1178 /label=BBa_B0034 misc_feature 1764..1795 /label=LVA CDS 1185..1763 /label=luxI misc_feature 1185..1802 /label=BBa_C0061 misc_feature 1803..1827 /label=Help:Barcodes CDS 1185..1802 /label=BBa_C0061 stem_loop 1847..1890 /label=stem_loop misc_feature 1836..1915 /label=BBa_B0010 terminator 1836..1915 /label=BBa_B0010 stem_loop 1931..1950 /label=T7 TE misc_feature 1957..1957 /label=stop polyA_site 1951..1964 /label=polya misc_feature 1924..1964 /label=BBa_B0012 terminator 1924..1964 /label=BBa_B0012 terminator 1836..1964 /label=BBa_B0015 misc_feature 1973..1992 /label=LuxR/HSL promoter 2087..2094 /label=-10 promoter 2061..2066 /label=-35 misc_feature 2100..2100 /label=start misc_feature 2102..2121 /label=Putative LuxR/HSL misc_feature 1973..2123 /label=BBa_R0063 promoter 1973..2123 /label=BBa_R0063 misc_feature 2136..2139 /label=conserved RBS 2132..2143 /label=BBa_B0034 misc_feature 2150..2151 /label=prefix misc_feature 2323..2323 /label=T misc_feature 2641..2641 /label=A CDS 2150..2899 /label=luxR misc_feature 2150..2905 /label=BBa_C0062 misc_feature 2906..2930 /label=Help:Barcodes CDS 2150..2905 /label=BBa_C0062 stem_loop 2950..2993 /label=stem_loop misc_feature 2939..3018 /label=BBa_B0010 terminator 2939..3018 /label=BBa_B0010 stem_loop 3034..3053 /label=T7 TE misc_feature 3060..3060 /label=stop polyA_site 3054..3067 /label=polya misc_feature 3027..3067 /label=BBa_B0012 terminator 3027..3067 /label=BBa_B0012 terminator 2939..3067 /label=BBa_B0015 misc_feature 3076..3095 /label=LuxR/HSL promoter 3095..3100 /label=-35 promoter 3117..3122 /label=-10 misc_feature 3128..3128 /label=start misc_feature 3076..3130 /label=BBa_R0062 promoter 3076..3130 /label=BBa_R0062 RBS 3139..3151 /label=RBS-3\Weak RBS 3145..3148 /label=RBS misc_feature 3139..3151 /label=BBa_B0032 RBS 3139..3151 /label=BBa_B0032 misc_feature 3158..3160 /label=start misc_feature 3941..3946 /label=2 misc_feature 3908..3940 /label=LVA CDS 3158..3907 /label=aiiA misc_feature 3158..3946 /label=BBa_C0060 misc_feature 3947..3971 /label=Help:Barcodes CDS 3158..3946 /label=BBa_C0060 stem_loop 3991..4034 /label=stem_loop misc_feature 3980..4059 /label=BBa_B0010 terminator 3980..4059 /label=BBa_B0010 stem_loop 4075..4094 /label=T7 TE misc_feature 4101..4101 /label=stop polyA_site 4095..4108 /label=polya misc_feature 4068..4108 /label=BBa_B0012 terminator 4068..4108 /label=BBa_B0012 terminator 3980..4108 /label=BBa_B0015 misc_feature 4117..4136 /label=LuxR/HSL promoter 4136..4141 /label=-35 promoter 4158..4163 /label=-10 misc_feature 4169..4169 /label=start misc_feature 4117..4171 /label=BBa_R0062 promoter 4117..4171 /label=BBa_R0062 misc_feature 4184..4187 /label=conserved RBS 4180..4191 /label=BBa_B0034 CDS 4198..4713 /label=BBa_K091109 stem_loop 4733..4776 /label=stem_loop misc_feature 4722..4801 /label=BBa_B0010 terminator 4722..4801 /label=BBa_B0010 stem_loop 4817..4836 /label=T7 TE misc_feature 4843..4843 /label=stop polyA_site 4837..4850 /label=polya misc_feature 4810..4850 /label=BBa_B0012 terminator 4810..4850 /label=BBa_B0012 terminator 4722..4850 /label=BBa_B0015 ORIGIN 1 acctgtacga tcctacaggt gcttatgtta agtaattgta ttcccagcga tacaatagtg 61 tgacaaaaat ccaatttatt agaatcaaat gtcaatccat taccgtttta atgatatata 121 acacgcaaaa cttgcgacaa acaataggta atactagaga aagaggagaa atactagatg 181 aaaaacataa atgccgacga cacatacaga ataattaata aaattaaagc ttgtagaagc 241 aataatgata ttaatcaatg cttatctgat atgactaaaa tggtacattg tgaatattat 301 ttactcgcga tcatttatcc tcattctatg gttaaatctg atatttcaat cctagataat 361 taccctaaaa aatggaggca atattatgat gacgctaatt taataaaata tgatcctata 421 gtagattatt ctaactccaa tcattcacca attaattgga atatatttga aaacaatgct 481 gtaaataaaa aatctccaaa tgtaattaaa gaagcgaaaa catcaggtct tatcactggg 541 tttagtttcc ctattcatac ggctaacaat ggcttcggaa tgcttagttt tgcacattca 601 gaaaaagaca actatataga tagtttattt ttacatgcgt gtatgaacat accattaatt 661 gttccttctc tagttgataa ttatcgaaaa ataaatatag caaataataa atcaaacaac 721 gatttaacca aaagagaaaa agaatgttta gcgtgggcat gcgaaggaaa aagctcttgg 781 gatatttcaa aaatattagg ttgcagtgag cgtactgtca ctttccattt aaccaatgcg 841 caaatgaaac tcaatacaac aaaccgctgc caaagtattt ctaaagcaat tttaacagga 901 gcaattgatt gcccatactt taaaaattaa taacactgat agtgctagtg tagatcacta 961 ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta 1021 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc 1081 ctttctgcgt ttatatacta gagacctgta ggatcgtaca ggtttacgca agaaaatggt 1141 ttgttatagt cgaataaata ctagagaaag aggagaaata ctagatgact ataatgataa 1201 aaaaatcgga ttttttggca attccatcgg aggagtataa aggtattcta agtcttcgtt 1261 atcaagtgtt taagcaaaga cttgagtggg acttagttgt agaaaataac cttgaatcag 1321 atgagtatga taactcaaat gcagaatata tttatgcttg tgatgatact gaaaatgtaa 1381 gtggatgctg gcgtttatta cctacaacag gtgattatat gctgaaaagt gtttttcctg 1441 aattgcttgg tcaacagagt gctcccaaag atcctaatat agtcgaatta agtcgttttg 1501 ctgtaggtaa aaatagctca aagataaata actctgctag tgaaattaca atgaaactat 1561 ttgaagctat atataaacac gctgttagtc aaggtattac agaatatgta acagtaacat 1621 caacagcaat agagcgattt ttaaagcgta ttaaagttcc ttgtcatcgt attggagaca 1681 aagaaattca tgtattaggt gatactaaat cggttgtatt gtctatgcct attaatgaac 1741 agtttaaaaa agcagtctta aatgctgcaa acgacgaaaa ctacgcttta gtagcttaat 1801 aactctgata gtgctagtgt agatctctac tagagccagg catcaaataa aacgaaaggc 1861 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta 1921 gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tatatactag agacctgtac 1981 gatcctacag gtgcttatgt taagtaattg tattcccagc gatacaatag tgtgacaaaa 2041 atccaattta ttagaatcaa atgtcaatcc attaccgttt taatgatata taacacgcaa 2101 aacttgcgac aaacaatagg taatactaga gaaagaggag aaatactaga tgaaaaacat 2161 aaatgccgac gacacataca gaataattaa taaaattaaa gcttgtagaa gcaataatga 2221 tattaatcaa tgcttatctg atatgactaa aatggtacat tgtgaatatt atttactcgc 2281 gatcatttat cctcattcta tggttaaatc tgatatttca atcctagata attaccctaa 2341 aaaatggagg caatattatg atgacgctaa tttaataaaa tatgatccta tagtagatta 2401 ttctaactcc aatcattcac caattaattg gaatatattt gaaaacaatg ctgtaaataa 2461 aaaatctcca aatgtaatta aagaagcgaa aacatcaggt cttatcactg ggtttagttt 2521 ccctattcat acggctaaca atggcttcgg aatgcttagt tttgcacatt cagaaaaaga 2581 caactatata gatagtttat ttttacatgc gtgtatgaac ataccattaa ttgttccttc 2641 tctagttgat aattatcgaa aaataaatat agcaaataat aaatcaaaca acgatttaac 2701 caaaagagaa aaagaatgtt tagcgtgggc atgcgaagga aaaagctctt gggatatttc 2761 aaaaatatta ggttgcagtg agcgtactgt cactttccat ttaaccaatg cgcaaatgaa 2821 actcaataca acaaaccgct gccaaagtat ttctaaagca attttaacag gagcaattga 2881 ttgcccatac tttaaaaatt aataacactg atagtgctag tgtagatcac tactagagcc 2941 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt 3001 ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc 3061 gtttatatac tagagacctg taggatcgta caggtttacg caagaaaatg gtttgttata 3121 gtcgaataaa tactagagtc acacaggaaa gtactagatg acagtaaaga agctttattt 3181 cgtcccagca ggtcgttgta tgttggatca ttcgtctgtt aatagtacat taacaccagg 3241 agaattatta gacttaccgg tttggtgtta tcttttggag actgaagaag gacctatttt 3301 agtagataca ggtatgccag aaagtgcagt taataatgaa ggtcttttta acggtacatt 3361 tgtcgaaggg caggttttac cgaaaatgac tgaagaagat agaatcgtga atattttaaa 3421 acgggttggt tatgagccgg aagaccttct ttatattatt agttctcact tgcattttga 3481 tcatgcagga ggaaatggcg cttttataaa tacaccaatc attgtacagc gtgctgaata 3541 tgaggcggcg cagcatagcg aagaatattt gaaagaatgt atattgccga atttaaacta 3601 caaaatcatt gaaggtgatt atgaagtcgt accaggagtt caattattgc atacaccagg 3661 ccatactcca gggcatcaat cgctattaat tgagacagaa aaatccggtc ctgtattatt 3721 aacgattgat gcatcgtata cgaaagagaa ttttgaaaat gaagtgccat ttgcgggatt 3781 tgattcagaa ttagctttat cttcaattaa acgtttaaaa gaagtggtga tgaaagagaa 3841 gccgattgtt ttctttggac atgatataga gcaggaaagg ggatgtaaag tgttccctga 3901 atatatagct gcaaacgacg aaaactacgc tttagtagct taataacgct gatagtgcta 3961 gtgtagatcg ctactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg 4021 gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca 4081 ccttcgggtg ggcctttctg cgtttatata ctagagacct gtaggatcgt acaggtttac 4141 gcaagaaaat ggtttgttat agtcgaataa atactagaga aagaggagaa atactagatg 4201 ccgttgttag atagcttcac agtcgatcat acccggatgg aagcgcctgc tgttcgggtg 4261 gcgaaaacaa tgaacacccc gcatggcgac gcaatcaccg tgttcgatct gcgcttctgc 4321 gtgccgaaca aagaagtgat gccagaaaga gggatccata ccctggagca cctgtttgct 4381 ggttttatgc gtaaccatct taacggtaat ggtgtagaga ttatcgatat ctcgccaatg 4441 ggctgccgca ccggttttta tatgagtctg attggtacgc cagatgagca gcgtgttgct 4501 gatgcctgga aagcggcaat ggaagacgtg ctgaaagtgc aggatcagaa tcagatcccg 4561 gaactgaacg tctaccagtg tggcacttac cagatgcact cgttgcagga agcgcaggat 4621 attgcgcgta gcattctgga acgtgacgta cgcatcaaca gcaacgaaga actggcactg 4681 ccgaaagaga agttgcagga actgcacatc taatactaga gccaggcatc aaataaaacg 4741 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct 4801 ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata //