##gff-version 3
##sequence-region BBa_K1886002 1 4850
BBa_K1886002	.	ribosome_entry_site	3139	3151	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_K1886002	.	ribosome_entry_site	3139	3151	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K1886002	.	engineered_region	3139	3151	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K1886002	.	ribosome_entry_site	3145	3148	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K1886002	.	promoter	1	151	.	+	0	ID=BBa_R0063;Name=BBa_R0063
BBa_K1886002	.	engineered_region	1	151	.	+	0	ID=annotation7071;Name=BBa_R0063;Parent=BBa_R0063
BBa_K1886002	.	start_codon	128	128	.	+	0	ID=annotation2054;Name=start;Parent=BBa_R0063
BBa_K1886002	.	promoter	115	122	.	+	0	ID=annotation2052;Name=-10;Parent=BBa_R0063
BBa_K1886002	.	non_covalent_binding_site	1	20	.	+	0	ID=annotation2051;Name=LuxR/HSL;Parent=BBa_R0063
BBa_K1886002	.	non_covalent_binding_site	130	149	.	+	0	ID=annotation2055;Name=Putative LuxR/HSL;Parent=BBa_R0063
BBa_K1886002	.	promoter	89	94	.	+	0	ID=annotation2053;Name=-35;Parent=BBa_R0063
BBa_K1886002	.	CDS	2150	2905	.	+	0	ID=BBa_C0062;Name=BBa_C0062
BBa_K1886002	.	engineered_region	2150	2151	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K1886002	.	engineered_region	2150	2905	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K1886002	.	engineered_tag	2906	2930	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K1886002	.	point_mutation	2641	2641	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K1886002	.	point_mutation	2323	2323	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K1886002	.	CDS	2150	2899	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K1886002	.	promoter	4117	4171	.	+	0	ID=BBa_R0062;Name=BBa_R0062
BBa_K1886002	.	non_covalent_binding_site	4117	4136	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_K1886002	.	promoter	4136	4141	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_K1886002	.	engineered_region	4117	4171	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_K1886002	.	promoter	4158	4163	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_K1886002	.	start_codon	4169	4169	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_K1886002	.	CDS	3158	3946	.	+	0	ID=BBa_C0060;Name=BBa_C0060
BBa_K1886002	.	engineered_tag	3947	3971	.	+	0	ID=annotation2213987;Name=Help:Barcodes;Parent=BBa_C0060
BBa_K1886002	.	CDS	3158	3907	.	+	0	ID=annotation1757;Name=aiiA;Parent=BBa_C0060
BBa_K1886002	.	tag	3908	3940	.	+	0	ID=annotation1756;Name=LVA;Parent=BBa_C0060
BBa_K1886002	.	stop_codon	3941	3946	.	+	0	ID=annotation1755;Name=2;Parent=BBa_C0060
BBa_K1886002	.	engineered_region	3158	3946	.	+	0	ID=annotation7037;Name=BBa_C0060;Parent=BBa_C0060
BBa_K1886002	.	start_codon	3158	3160	.	+	0	ID=annotation1754;Name=start;Parent=BBa_C0060
BBa_K1886002	.	terminator	1836	1964	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1886002	.	terminator	1924	1964	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1886002	.	engineered_region	1924	1964	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1886002	.	stem_loop	1931	1950	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1886002	.	polyA_site	1951	1964	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1886002	.	stop_codon	1957	1957	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1886002	.	terminator	1836	1915	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1886002	.	engineered_region	1836	1915	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1886002	.	stem_loop	1847	1890	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1886002	.	promoter	1104	1158	.	+	0	ID=BBa_R0062;Name=BBa_R0062
BBa_K1886002	.	non_covalent_binding_site	1104	1123	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_K1886002	.	promoter	1123	1128	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_K1886002	.	engineered_region	1104	1158	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_K1886002	.	promoter	1145	1150	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_K1886002	.	start_codon	1156	1156	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_K1886002	.	terminator	967	1095	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1886002	.	terminator	1055	1095	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1886002	.	engineered_region	1055	1095	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1886002	.	stem_loop	1062	1081	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1886002	.	polyA_site	1082	1095	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1886002	.	stop_codon	1088	1088	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1886002	.	terminator	967	1046	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1886002	.	engineered_region	967	1046	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1886002	.	stem_loop	978	1021	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1886002	.	CDS	1185	1802	.	+	0	ID=BBa_C0061;Name=BBa_C0061
BBa_K1886002	.	CDS	1185	1763	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_K1886002	.	tag	1764	1795	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_K1886002	.	engineered_tag	1803	1827	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_K1886002	.	engineered_region	1185	1802	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_K1886002	.	ribosome_entry_site	160	171	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K1886002	.	conserved_region	164	167	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1886002	.	terminator	4722	4850	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1886002	.	terminator	4810	4850	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1886002	.	engineered_region	4810	4850	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1886002	.	stem_loop	4817	4836	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1886002	.	polyA_site	4837	4850	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1886002	.	stop_codon	4843	4843	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1886002	.	terminator	4722	4801	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1886002	.	engineered_region	4722	4801	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1886002	.	stem_loop	4733	4776	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1886002	.	terminator	2939	3067	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1886002	.	terminator	3027	3067	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1886002	.	engineered_region	3027	3067	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1886002	.	stem_loop	3034	3053	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1886002	.	polyA_site	3054	3067	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1886002	.	stop_codon	3060	3060	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1886002	.	terminator	2939	3018	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1886002	.	engineered_region	2939	3018	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1886002	.	stem_loop	2950	2993	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1886002	.	CDS	178	933	.	+	0	ID=BBa_C0062;Name=BBa_C0062
BBa_K1886002	.	engineered_region	178	179	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K1886002	.	engineered_region	178	933	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K1886002	.	engineered_tag	934	958	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K1886002	.	point_mutation	669	669	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K1886002	.	point_mutation	351	351	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K1886002	.	CDS	178	927	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K1886002	.	promoter	3076	3130	.	+	0	ID=BBa_R0062;Name=BBa_R0062
BBa_K1886002	.	non_covalent_binding_site	3076	3095	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_K1886002	.	promoter	3095	3100	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_K1886002	.	engineered_region	3076	3130	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_K1886002	.	promoter	3117	3122	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_K1886002	.	start_codon	3128	3128	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_K1886002	.	ribosome_entry_site	2132	2143	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K1886002	.	conserved_region	2136	2139	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1886002	.	terminator	3980	4108	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1886002	.	terminator	4068	4108	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1886002	.	engineered_region	4068	4108	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1886002	.	stem_loop	4075	4094	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1886002	.	polyA_site	4095	4108	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1886002	.	stop_codon	4101	4101	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1886002	.	terminator	3980	4059	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1886002	.	engineered_region	3980	4059	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1886002	.	stem_loop	3991	4034	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1886002	.	ribosome_entry_site	1167	1178	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K1886002	.	conserved_region	1171	1174	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1886002	.	CDS	4198	4713	.	+	0	ID=BBa_K091109;Name=BBa_K091109
BBa_K1886002	.	ribosome_entry_site	4180	4191	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K1886002	.	conserved_region	4184	4187	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1886002	.	promoter	1973	2123	.	+	0	ID=BBa_R0063;Name=BBa_R0063
BBa_K1886002	.	engineered_region	1973	2123	.	+	0	ID=annotation7071;Name=BBa_R0063;Parent=BBa_R0063
BBa_K1886002	.	start_codon	2100	2100	.	+	0	ID=annotation2054;Name=start;Parent=BBa_R0063
BBa_K1886002	.	promoter	2087	2094	.	+	0	ID=annotation2052;Name=-10;Parent=BBa_R0063
BBa_K1886002	.	non_covalent_binding_site	1973	1992	.	+	0	ID=annotation2051;Name=LuxR/HSL;Parent=BBa_R0063
BBa_K1886002	.	non_covalent_binding_site	2102	2121	.	+	0	ID=annotation2055;Name=Putative LuxR/HSL;Parent=BBa_R0063
BBa_K1886002	.	promoter	2061	2066	.	+	0	ID=annotation2053;Name=-35;Parent=BBa_R0063
>BBa_K1886002
acctgtacgatcctacaggtgcttatgttaagtaattgtattcccagcgatacaatagtgtgacaaaaatccaatttat
tagaatcaaatgtcaatccattaccgttttaatgatatataacacgcaaaacttgcgacaaacaataggtaatactaga
gaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtaga
agcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcattt
atcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgc
taatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaac
aatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttcccta
ttcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttattttt
acatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaa
tcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaa
aaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctg
ccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctag
tgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttg
tttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacc
tgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagagaaagaggagaaatactaga
tgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatca
agtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgca
gaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgc
tgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgc
tgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacac
gctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttc
cttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatga
acagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctag
tgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttg
tttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacc
tgtacgatcctacaggtgcttatgttaagtaattgtattcccagcgatacaatagtgtgacaaaaatccaatttattag
aatcaaatgtcaatccattaccgttttaatgatatataacacgcaaaacttgcgacaaacaataggtaatactagagaa
agaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagc
aataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatc
ctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaa
tttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaat
gctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattc
atacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttaca
tgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatca
aacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaa
tattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgcca
aagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgt
agatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgttt
gtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgt
aggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagagtcacacaggaaagtactagatg
acagtaaagaagctttatttcgtcccagcaggtcgttgtatgttggatcattcgtctgttaatagtacattaacaccag
gagaattattagacttaccggtttggtgttatcttttggagactgaagaaggacctattttagtagatacaggtatgcc
agaaagtgcagttaataatgaaggtctttttaacggtacatttgtcgaagggcaggttttaccgaaaatgactgaagaa
gatagaatcgtgaatattttaaaacgggttggttatgagccggaagaccttctttatattattagttctcacttgcatt
ttgatcatgcaggaggaaatggcgcttttataaatacaccaatcattgtacagcgtgctgaatatgaggcggcgcagca
tagcgaagaatatttgaaagaatgtatattgccgaatttaaactacaaaatcattgaaggtgattatgaagtcgtacca
ggagttcaattattgcatacaccaggccatactccagggcatcaatcgctattaattgagacagaaaaatccggtcctg
tattattaacgattgatgcatcgtatacgaaagagaattttgaaaatgaagtgccatttgcgggatttgattcagaatt
agctttatcttcaattaaacgtttaaaagaagtggtgatgaaagagaagccgattgttttctttggacatgatatagag
caggaaaggggatgtaaagtgttccctgaatatatagctgcaaacgacgaaaactacgctttagtagcttaataacgct
gatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgt
tttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
tactagagacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagagaaagagga
gaaatactagatgccgttgttagatagcttcacagtcgatcatacccggatggaagcgcctgctgttcgggtggcgaaa
acaatgaacaccccgcatggcgacgcaatcaccgtgttcgatctgcgcttctgcgtgccgaacaaagaagtgatgccag
aaagagggatccataccctggagcacctgtttgctggttttatgcgtaaccatcttaacggtaatggtgtagagattat
cgatatctcgccaatgggctgccgcaccggtttttatatgagtctgattggtacgccagatgagcagcgtgttgctgat
gcctggaaagcggcaatggaagacgtgctgaaagtgcaggatcagaatcagatcccggaactgaacgtctaccagtgtg
gcacttaccagatgcactcgttgcaggaagcgcaggatattgcgcgtagcattctggaacgtgacgtacgcatcaacag
caacgaagaactggcactgccgaaagagaagttgcaggaactgcacatctaatactagagccaggcatcaaataaaacg
aaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggc
tcaccttcgggtgggcctttctgcgtttata