BBa_K1886004 1 BBa_K1886004 sll1472 ccaS 2016-10-05T11:00:00Z 2016-10-17T12:57:05Z Refactoring and Optimization of Light-Switchable Escherichia coli Two-Component Systems This circuit is composed of the constitutive promoter and photosensitive protein gene ccaS . ccaS protein is membrane protein. After being activated by 520-nm light, it could transfer its phosphoryl group to the intracellular protein ccaR,and this process could be inhibited by 650-nm light false false _2351_ 29387 29387 9 false It's not difficult to construct this part,but the strength of the promoter and the RBS will influence the abundance of protein ccaS,and finally influence the whole TCS.And there was a SpeI site and a EcoRI site,so we designed two silent mutations.The upstream of gene ccaS called sll1472 is of unknown function. false Lejian Jiang annotation2514809 1 ccaS range2514809 1 472 2733 annotation2514810 1 unknown area range2514810 1 1 471 BBa_K1886004_sequence 1 atgttgaaactagccggtaaaaaagcctctgccctgacctgcaagaaaacgaaggcaaaaattggtatcctagaaatggatcggcagtggtatcaatcagtcaagcgatttttatgtccaagttttgaaatttctgctatcaacttccaagatattttgtttaacaatgttgacgttggagaatatcattcgatcctagttggttgcgggattaaatatgaatcaattgatatttcagctactttagatttagtgaccattattaaaaaattgtctgctaagccgccggccctactactaattacggattgtgccgacagtcagatcacggtacaagctcgcagttacctgccccaaattgacggcgtttttgccaaggatcatgatttagctttattgttaaaagtcatgaaaattattgccaaacaaaaatattttaagtaaggtttttctactgggattcgctaatatatgggcaaatttctaattccaatcgaatttgtttttctggcgatcgccatgacctgttatttatggcacagacaaaaccaagaacgccgcaggattgaaattagcatcaagcaacaaacccaacgggaacgatttattaaccaaattacccaacatatccgccaatctttaaacttggaaacggttttaaataccaccgtcgctgaagttaaaaccctgttgcaagttgatcgagttctaatttatcgcatttggcaagatggcacgggcagcgccattacggaatcggtgaatgccaattatcctagtattttagggcggaccttttccgatgaagtttttcccgttgaataccatcaagcctacaccaaaggtaaagtacgggccattaatgacattgaccaggatgacatagagatttgcctagctgatttcgtcaaacaatttggcgtgaaatcaaaattagtagtgcccattcttcaacataatcgtgcttcttccctagataatgaatcagaatttccctatctttgggggctgttaattacccatcaatgtgcttttacccggccatggcaaccgtgggaagtggagttaatgaaacagctagccaatcaggtcgcgatcgccatccaacaatcggaattatatgagcaattacagcaactcaataaagatttggaaaaccgagtcgaaaaacgcacccagcaacttgccgccaccaatcaatccctaagaatggaaatcagtgagcgacaaaaaacggaagccgctctccgccacactaaccatactctgcaatccctgattgcggcctcccccaggggtatttttacccttaatttagcagaccaaattcagatttggaatcctacagcagaacgtatttttggttggacagaaacagaaattattgcccatccagaattattaacatccaacattttgctggaagattatcagcaatttaaacagaaagttttatcaggcatggtttcccctagcctagaattaaaatgtcaaaaaaaagatggtagttggattgaaattgtcctttccgctgctcccctattggatagtgaagaaaatattgccggattggtggcggttgtcgccgatattaccgagcaaaagcggcaggcagaacaaattcgtttgctacaatccgttgtggttaatactaatgatgcggtggtgattacggaagcggagcccattgatgatcccgggccgcgcattctctatgtcaatgaagcatttactaaaatcaccggttatactgctgaagaaatgctaggcaaaaccccccgagttttacagggaccaaaaactagccgcactgaattagatagggtgcggcaagccattagtcaatggcaatcagttaccgttgaagtgattaattatcgtaaggatggcagtgagttttgggtggaatttagtctggtgcccgttgccaataaaacaggtttttacacccattggattgctgtgcaaagggatgtcactgagcgccgacgcacggaggaagtccgcctagctttagaacgggaaaaagaattaagccgcctaaaaactcgttttttctccatggcttcccatgaatttcgtactcccctcagtacggccttagctgctgcccaattactggaaaattctgaagtggcctggcttgatcccgataagcgtagccggaacttacaccgtattcaaaattccgtgaaaaatatggtacagctcctggatgatattttaatcattaaccgtgccgaagcgggcaaattggaatttaatcctaattggttagatttgaaattattgttccagcaatttatcgaagaaattcaattaagtgtcagtgaccaatattattttgactttatttgtagcgctcaagatacgaaggcattggtggatgaaaggttagtgcggtctattttatctaatctgttatctaatgcgattaaatactctcccgggggagggcagattaaaattgccctaagcctagattcggaacagattatttttgaagtcaccgaccagggcattggcatttcgccagaggaccaaaagcaaatttttgaaccctttcatcggggcaaaaatgtcagaaatattacgggaacaggactcggtttaatggttgccaagaaatgtgttgacttacacagtggcagtatcttgctaaaaagtgcagttgaccagggaacaacagttactatctgtttaaaacgctataaccatttgcctcgagcttag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z