BBa_K1886004
1
BBa_K1886004
sll1472 ccaS
2016-10-05T11:00:00Z
2016-10-17T12:57:05Z
Refactoring and Optimization of Light-Switchable Escherichia coli Two-Component Systems
This circuit is composed of the constitutive promoter and photosensitive protein gene ccaS . ccaS protein is membrane protein. After being activated by 520-nm light, it could transfer its phosphoryl group to the intracellular protein ccaR,and this process could be inhibited by 650-nm light
false
false
_2351_
29387
29387
9
false
It's not difficult to construct this part,but the strength of the promoter and the RBS will influence the abundance of protein ccaS,and finally influence the whole TCS.And there was a SpeI site and a EcoRI site,so we designed two silent mutations.The upstream of gene ccaS called sll1472 is of unknown function.
false
Lejian Jiang
annotation2514810
1
unknown area
range2514810
1
1
471
annotation2514809
1
ccaS
range2514809
1
472
2733
BBa_K1886004_sequence
1
atgttgaaactagccggtaaaaaagcctctgccctgacctgcaagaaaacgaaggcaaaaattggtatcctagaaatggatcggcagtggtatcaatcagtcaagcgatttttatgtccaagttttgaaatttctgctatcaacttccaagatattttgtttaacaatgttgacgttggagaatatcattcgatcctagttggttgcgggattaaatatgaatcaattgatatttcagctactttagatttagtgaccattattaaaaaattgtctgctaagccgccggccctactactaattacggattgtgccgacagtcagatcacggtacaagctcgcagttacctgccccaaattgacggcgtttttgccaaggatcatgatttagctttattgttaaaagtcatgaaaattattgccaaacaaaaatattttaagtaaggtttttctactgggattcgctaatatatgggcaaatttctaattccaatcgaatttgtttttctggcgatcgccatgacctgttatttatggcacagacaaaaccaagaacgccgcaggattgaaattagcatcaagcaacaaacccaacgggaacgatttattaaccaaattacccaacatatccgccaatctttaaacttggaaacggttttaaataccaccgtcgctgaagttaaaaccctgttgcaagttgatcgagttctaatttatcgcatttggcaagatggcacgggcagcgccattacggaatcggtgaatgccaattatcctagtattttagggcggaccttttccgatgaagtttttcccgttgaataccatcaagcctacaccaaaggtaaagtacgggccattaatgacattgaccaggatgacatagagatttgcctagctgatttcgtcaaacaatttggcgtgaaatcaaaattagtagtgcccattcttcaacataatcgtgcttcttccctagataatgaatcagaatttccctatctttgggggctgttaattacccatcaatgtgcttttacccggccatggcaaccgtgggaagtggagttaatgaaacagctagccaatcaggtcgcgatcgccatccaacaatcggaattatatgagcaattacagcaactcaataaagatttggaaaaccgagtcgaaaaacgcacccagcaacttgccgccaccaatcaatccctaagaatggaaatcagtgagcgacaaaaaacggaagccgctctccgccacactaaccatactctgcaatccctgattgcggcctcccccaggggtatttttacccttaatttagcagaccaaattcagatttggaatcctacagcagaacgtatttttggttggacagaaacagaaattattgcccatccagaattattaacatccaacattttgctggaagattatcagcaatttaaacagaaagttttatcaggcatggtttcccctagcctagaattaaaatgtcaaaaaaaagatggtagttggattgaaattgtcctttccgctgctcccctattggatagtgaagaaaatattgccggattggtggcggttgtcgccgatattaccgagcaaaagcggcaggcagaacaaattcgtttgctacaatccgttgtggttaatactaatgatgcggtggtgattacggaagcggagcccattgatgatcccgggccgcgcattctctatgtcaatgaagcatttactaaaatcaccggttatactgctgaagaaatgctaggcaaaaccccccgagttttacagggaccaaaaactagccgcactgaattagatagggtgcggcaagccattagtcaatggcaatcagttaccgttgaagtgattaattatcgtaaggatggcagtgagttttgggtggaatttagtctggtgcccgttgccaataaaacaggtttttacacccattggattgctgtgcaaagggatgtcactgagcgccgacgcacggaggaagtccgcctagctttagaacgggaaaaagaattaagccgcctaaaaactcgttttttctccatggcttcccatgaatttcgtactcccctcagtacggccttagctgctgcccaattactggaaaattctgaagtggcctggcttgatcccgataagcgtagccggaacttacaccgtattcaaaattccgtgaaaaatatggtacagctcctggatgatattttaatcattaaccgtgccgaagcgggcaaattggaatttaatcctaattggttagatttgaaattattgttccagcaatttatcgaagaaattcaattaagtgtcagtgaccaatattattttgactttatttgtagcgctcaagatacgaaggcattggtggatgaaaggttagtgcggtctattttatctaatctgttatctaatgcgattaaatactctcccgggggagggcagattaaaattgccctaagcctagattcggaacagattatttttgaagtcaccgaccagggcattggcatttcgccagaggaccaaaagcaaatttttgaaccctttcatcggggcaaaaatgtcagaaatattacgggaacaggactcggtttaatggttgccaagaaatgtgttgacttacacagtggcagtatcttgctaaaaagtgcagttgaccagggaacaacagttactatctgtttaaaacgctataaccatttgcctcgagcttag
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z