LOCUS       BBa_K1886006            2387 bp    DNA     linear       17-Dec-2024
DEFINITION  broken Ptet-cph8
ACCESSION   BBa_K1886006
VERSION     BBa_K1886006.1  
FEATURES             Location/Qualifiers
     promoter        1..49
                     /label=BBa_K1886017
     misc_feature    62..65
                     /label=conserved
     RBS             58..69
                     /label=BBa_B0034
     CDS             76..2313
                     /label=Cph8
     misc_feature    2314..2338
                     /label=Help:Barcodes
     CDS             76..2338
                     /label=BBa_I15010
     stem_loop       2354..2373
                     /label=T7 TE
     misc_feature    2380..2380
                     /label=stop
     polyA_site      2374..2387
                     /label=polya
     misc_feature    2347..2387
                     /label=BBa_B0012
     terminator      2347..2387
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat actgagcaca tcagcaggac gcactgacct actagagaaa
       61 gaggagaaat actagatggc caccaccgta caactcagcg accaatccct ccgtcagcta
      121 gaaaccctcg ccatccacac cgcccacctg attcagcccc acggtttagt ggtggtcctg
      181 caagaaccag acctcaccat cagccaaatt agcgccaact gcaccggcat tttagggcga
      241 tcgccagagg atttgttggg cagaacccta ggggaagtgt ttgatagctt tcagattgat
      301 cccatccaga gtcgcctaac ggccggacaa atcagcagcc tcaaccccag taaactttgg
      361 gcgcgggtca tgggggacga ctttgtcatt tttgacgggg tttttcatcg caacagtgac
      421 ggtttattgg tatgtgaact cgagccagcc tacacttccg ataatctgcc cttcctcggt
      481 ttttatcaca tggccaacgc tgccctgaat cggttgcgcc aacaagctaa tctacgggat
      541 ttctacgatg ttattgtcga agaagtccgc cgtatgactg gctttgaccg ggtgatgcta
      601 taccgctttg atgaaaataa ccacggtgat gtcattgccg aagataaacg ggatgatatg
      661 gaaccctatt tgggcctgca ctatcccgaa tcggatattc cccaacccgc ccgtcggcta
      721 tttatccaca accccattcg agtaattccc gatgtttatg gtgtggcggt gcccctgacc
      781 ccagcggtta accccagcac caaccgagcg gtggatttaa cagaatccat tctgcgcagt
      841 gcgtaccatt gccacttgac ctatctgaaa aatatggggg taggagcgtc tttaaccatt
      901 tccctaatta aggacggcca tctctggggg ctcattgcct gccaccatca aacccccaaa
      961 gtaattccct ttgaactgcg taaagcctgc gaattttttg gtcgggtggt gtttagcaac
     1021 atttccgccc aggaagatac ggaaaccttc gattaccggg tgcagctggc ggagcatgaa
     1081 gcggttttat tggacaaaat gaccacggcg gcggattttg tcgaaggatt aactaatcat
     1141 cccgatcgcc tgttgggatt aacgggctcc cagggggcgg ccatttgctt tggggaaaaa
     1201 ttgattttag taggggaaac cccggacgag aaagcagtgc aatatttact gcaatggttg
     1261 gagaatcggg aagtgcaaga cgttttcttc acctcttccc tctcacaaat ttatcctgat
     1321 gcagtgaatt ttaaatccgt ggccagtggc ttattggcca ttcccattgc ccgtcacaac
     1381 tttttgctct ggtttcgccc tgaagtgttg caaacggtta attggggcgg tgacccaaat
     1441 catgcttacg aagctaccca ggaagacggt aaaatcgagc tccatccccg ccaatccttt
     1501 gacctctgga aagaaattgt ccgactccaa tctttgccct ggcaatcggt ggaaatccaa
     1561 agtgccctgg ccctgaaaaa ggcgatcgtc aacctcattt tgcgccaggc agaagaattg
     1621 catatggcgg ctggtgttaa gcaactggcg gatgaccgca cgctgctgat ggcgggggta
     1681 agtcacgact tgcgcacgcc gctgacgcgt attcgcctgg cgactgagat gatgagcgag
     1741 caggatggct atctggcaga atcgatcaat aaagatatcg aagagtgcaa cgccatcatt
     1801 gagcagttta tcgactacct gcgcaccggg caggagatgc cgatggaaat ggcggatctt
     1861 aatgcagtac tcggtgaggt gattgctgcc gaaagtggct atgagcggga aattgaaacc
     1921 gcgctttacc ccggcagcat tgaagtgaaa atgcacccgc tgtcgatcaa acgcgcggtg
     1981 gcgaatatgg tggtcaacgc cgcccgttat ggcaatggct gggtcaaagt cagcagcgga
     2041 acggagccga atcgcgcctg gttccaggtg gaagatgacg gtccgggaat tgcgccggaa
     2101 caacgtaagc acctgttcca gccgtttgtc cgcggcgaca gtgcgcgcac cattagcggc
     2161 acgggattag ggctggcaat tgtgcagcgt atcgtggata accataacgg gatgctggag
     2221 cttggcacca gcgagcgggg cgggctttcc attcgcgcct ggctgccagt gccggtaacg
     2281 cgggcgcagg gcatgacaaa agaagggtaa taactctgat agtgctagtg tagatctcta
     2341 ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttata
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