LOCUS       BBa_K1886010            3417 bp    DNA     linear       05-May-2025
DEFINITION  AND GATE-input_AHL+green light
ACCESSION   BBa_K1886010
VERSION     BBa_K1886010.1  
FEATURES             Location/Qualifiers
     promoter        1..238
                     /label=PcpcG2 promoter
     promoter        1..238
                     /label=BBa_K592003
     misc_feature    247..382
                     /label=BBa_K228001
     stem_loop       402..445
                     /label=stem_loop
     misc_feature    391..470
                     /label=BBa_B0010
     terminator      391..470
                     /label=BBa_B0010
     stem_loop       486..505
                     /label=T7 TE
     misc_feature    512..512
                     /label=stop
     polyA_site      506..519
                     /label=polya
     misc_feature    479..519
                     /label=BBa_B0012
     terminator      479..519
                     /label=BBa_B0012
     terminator      391..519
                     /label=BBa_B0015
     misc_feature    528..547
                     /label=LuxR/HSL
     promoter        547..552
                     /label=-35
     promoter        569..574
                     /label=-10
     misc_feature    580..580
                     /label=start
     misc_feature    528..582
                     /label=BBa_R0062
     promoter        528..582
                     /label=BBa_R0062
     RBS             591..601
                     /label=RBS-4\Weaker
     RBS             597..600
                     /label=RBS
     misc_feature    591..601
                     /label=BBa_B0033
     RBS             591..601
                     /label=BBa_B0033
     CDS             608..3280
                     /label=cds
     CDS             608..3280
                     /label=BBa_K228000
     stem_loop       3300..3343
                     /label=stem_loop
     misc_feature    3289..3368
                     /label=BBa_B0010
     terminator      3289..3368
                     /label=BBa_B0010
     stem_loop       3384..3403
                     /label=T7 TE
     misc_feature    3410..3410
                     /label=stop
     polyA_site      3404..3417
                     /label=polya
     misc_feature    3377..3417
                     /label=BBa_B0012
     terminator      3377..3417
                     /label=BBa_B0012
     terminator      3289..3417
                     /label=BBa_B0015
ORIGIN
        1 agcccattgt gcttttctct atcaacctca gcttacctga aggggtgaac aggtctgggt
       61 taattcatgt tgcgaaatgt aacagtttta gtcgcatcag ctaactttcc gatttcttta
      121 cgattttctc ccccttttct tcaattttac tttgttagga tcgcattttt aatgccaaca
      181 cataccagtt attggctgga cattaaacaa cttttaagtt taattactaa ctttatctta
      241 ctagagcaat tcggagagat gccggagcgg ctgaacggac cggtctctaa aaccggagta
      301 ggggcaactc taccgggggt tcaaatcccc ctctctccgc cactacagat ccttagcgaa
      361 agctaaggat tttttttaag cttactagag ccaggcatca aataaaacga aaggctcagt
      421 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc
      481 acactggctc accttcgggt gggcctttct gcgtttatat actagagacc tgtaggatcg
      541 tacaggttta cgcaagaaaa tggtttgtta tagtcgaata aatactagag tcacacagga
      601 ctactagatg accatgatta ccgtgcacta gaataccatt aacattgcta agaacgactt
      661 ctctgacatc gaactggctg ctatcccgtt caacactctg gctgaccatt acggtgagcg
      721 tttagctcgc gaacagttgg cccttgagca tgagtcttac gagatgggtg aagcacgctt
      781 ccgcaagatg tttgagcgtc aacttaaagc tggtgaggtt gcggataacg ctgccgccaa
      841 gcctctcatc actaccctac tccctaagat gattgcacgc atcaacgact ggtttgagga
      901 agtgaaagct aagcgcggca agcgcccgac agccttccag ttcctgtagg aaatcaagcc
      961 ggaagccgta gcgtacatca ccattaagac cactctggct tgcctaacca gtgctgacaa
     1021 tacaaccgtt caggctgtag caagcgcaat cggtcgggcc attgaggacg aggctcgctt
     1081 cggtcgtatc cgtgaccttg aagctaagca cttcaagaaa aacgttgagg aacaactcaa
     1141 caagcgcgta gggcacgtct acaagaaagc atttatgcaa gttgtcgagg ctgacatgct
     1201 ctctaagggt ctactcggtg gcgaggcgtg gtcttcgtgg cataaggaag actctattca
     1261 tgtaggagta cgctgcatcg agatgctcat tgagtcaacc ggaatggtta gcttacaccg
     1321 ccaaaatgct ggcgtagtag gtcaagactc tgagactatc gaactcgcac ctgaatacgc
     1381 tgaggctatc gcaacccgtg caggtgcgct ggctggcatc tctccgatgt tccaaccttg
     1441 cgtagttcct cctaagccgt ggactggcat tactggtggt ggctattggg ctaacggtcg
     1501 tcgtcctctg gcgctggtgc gtactcacag taagaaagca ctgatgcgct acgaagacgt
     1561 ttacatgcct gaggtgtaca aagcgattaa cattgcgcaa aacaccgcat ggaaaatcaa
     1621 caagaaagtc ctagcggtcg ccaacgtaat caccaagtgg aagcattgtc cggtcgagga
     1681 catccctgcg attgagcgtg aagaactccc gatgaaaccg gaagacatcg acatgaatcc
     1741 tgaggctctc accgcgtgga aacgtgctgc cgctgctgtg taccgcaagg acaaggctcg
     1801 caagtctcgc cgtatcagcc ttgagttcat gcttgagcaa gccaataagt ttgctaacca
     1861 taaggccatc tggttccctt acaacatgga ctggcgcggt cgtgtttacg ctgtgtcaat
     1921 gttcaacccg caaggtaacg atatgaccaa aggactgctt acgctggcga aaggtaaacc
     1981 aatcggtaag gaaggttact actggctgaa aatccacggt gcaaactgtg cgggtgtcga
     2041 taaggttccg ttccctgagc gcatcaagtt cattgaggaa aaccacgaga acatcatggc
     2101 ttgcgctaag tctccactgg agaacacttg gtgggctgag caagattctc cgttctgctt
     2161 ccttgcgttc tgctttgagt acgctggggt acagcaccac ggcctgagct ataactgctc
     2221 ccttccgctg gcgtttgacg ggtcttgctc tggcatccag cacttctccg cgatgctccg
     2281 agatgaggta ggtggtcgcg cggttaactt gcttcctagt gaaaccgttc aggacatcta
     2341 cgggattgtt gctaagaaag tcaacgagat tctacaagca gacgcaatca atgggaccga
     2401 taacgaagta gttaccgtga ccgatgagaa cactggtgaa atctctgaga aagtcaagct
     2461 gggcactaag gcactggctg gtcaatggct ggcttacggt gttactcgca gtgtgactaa
     2521 gcgttcagtc atgacgctgg cttacgggtc caaagagttc ggcttccgtc aacaagtgct
     2581 ggaagatacc attcagccag ctattgattc cggcaagggt ctgatgttca ctcagccgaa
     2641 tcaggctgct ggatacatgg ctaagctgat ttgggaatct gtgagcgtga cggtggtagc
     2701 tgcggttgaa gcaatgaact ggcttaagtc tgctgctaag ctgctggctg ctgaggtcaa
     2761 agataagaag actggagaga ttcttcgcaa gcgttgcgct gtgcattggg taactcctga
     2821 tggtttccct gtgtggcagg aatacaagaa gcctattcag acgcgcttga acctgatgtt
     2881 cctcggtcag ttccgcttac agcctaccat taacaccaac aaagatagcg agattgatgc
     2941 acacaaacag gagtctggta tcgctcctaa ctttgtacac agccaagacg gtagccacct
     3001 tcgtaagact gtagtgtggg cacacgagaa gtacggaatc gaatcttttg cactgattca
     3061 cgactccttc ggtaccattc cggctgacgc tgcgaacctg ttcaaagcag tgcgcgaaac
     3121 tatggttgac acatatgagt cttgtgatgt actggctgat ttctacgacc agttcgctga
     3181 ccagttgcac gagtctcaat tggacaaaat gccagcactt ccggctaaag gtaacttgaa
     3241 cctccgtgac atcttagagt cggacttcgc gttcgcataa tactagagcc aggcatcaaa
     3301 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga
     3361 acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttata
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