LOCUS       BBa_K1886011            3247 bp    DNA     linear       22-Nov-2024
DEFINITION  AND GATE-input_AHL+red light
ACCESSION   BBa_K1886011
VERSION     BBa_K1886011.1  
FEATURES             Location/Qualifiers
     promoter        1..68
                     /label=BBa_K1145005
     misc_feature    77..212
                     /label=BBa_K228001
     stem_loop       232..275
                     /label=stem_loop
     misc_feature    221..300
                     /label=BBa_B0010
     terminator      221..300
                     /label=BBa_B0010
     stem_loop       316..335
                     /label=T7 TE
     misc_feature    342..342
                     /label=stop
     polyA_site      336..349
                     /label=polya
     misc_feature    309..349
                     /label=BBa_B0012
     terminator      309..349
                     /label=BBa_B0012
     terminator      221..349
                     /label=BBa_B0015
     misc_feature    358..377
                     /label=LuxR/HSL
     promoter        377..382
                     /label=-35
     promoter        399..404
                     /label=-10
     misc_feature    410..410
                     /label=start
     misc_feature    358..412
                     /label=BBa_R0062
     promoter        358..412
                     /label=BBa_R0062
     RBS             421..431
                     /label=RBS-4\Weaker
     RBS             427..430
                     /label=RBS
     misc_feature    421..431
                     /label=BBa_B0033
     RBS             421..431
                     /label=BBa_B0033
     CDS             438..3110
                     /label=cds
     CDS             438..3110
                     /label=BBa_K228000
     stem_loop       3130..3173
                     /label=stem_loop
     misc_feature    3119..3198
                     /label=BBa_B0010
     terminator      3119..3198
                     /label=BBa_B0010
     stem_loop       3214..3233
                     /label=T7 TE
     misc_feature    3240..3240
                     /label=stop
     polyA_site      3234..3247
                     /label=polya
     misc_feature    3207..3247
                     /label=BBa_B0012
     terminator      3207..3247
                     /label=BBa_B0012
     terminator      3119..3247
                     /label=BBa_B0015
ORIGIN
        1 ggttcctagt aaatatctaa caccgtgcgt gttgactatt ttacctctgg cggtgataat
       61 ggttgcatta ctagagcaat tcggagagat gccggagcgg ctgaacggac cggtctctaa
      121 aaccggagta ggggcaactc taccgggggt tcaaatcccc ctctctccgc cactacagat
      181 ccttagcgaa agctaaggat tttttttaag cttactagag ccaggcatca aataaaacga
      241 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
      301 tactagagtc acactggctc accttcgggt gggcctttct gcgtttatat actagagacc
      361 tgtaggatcg tacaggttta cgcaagaaaa tggtttgtta tagtcgaata aatactagag
      421 tcacacagga ctactagatg accatgatta ccgtgcacta gaataccatt aacattgcta
      481 agaacgactt ctctgacatc gaactggctg ctatcccgtt caacactctg gctgaccatt
      541 acggtgagcg tttagctcgc gaacagttgg cccttgagca tgagtcttac gagatgggtg
      601 aagcacgctt ccgcaagatg tttgagcgtc aacttaaagc tggtgaggtt gcggataacg
      661 ctgccgccaa gcctctcatc actaccctac tccctaagat gattgcacgc atcaacgact
      721 ggtttgagga agtgaaagct aagcgcggca agcgcccgac agccttccag ttcctgtagg
      781 aaatcaagcc ggaagccgta gcgtacatca ccattaagac cactctggct tgcctaacca
      841 gtgctgacaa tacaaccgtt caggctgtag caagcgcaat cggtcgggcc attgaggacg
      901 aggctcgctt cggtcgtatc cgtgaccttg aagctaagca cttcaagaaa aacgttgagg
      961 aacaactcaa caagcgcgta gggcacgtct acaagaaagc atttatgcaa gttgtcgagg
     1021 ctgacatgct ctctaagggt ctactcggtg gcgaggcgtg gtcttcgtgg cataaggaag
     1081 actctattca tgtaggagta cgctgcatcg agatgctcat tgagtcaacc ggaatggtta
     1141 gcttacaccg ccaaaatgct ggcgtagtag gtcaagactc tgagactatc gaactcgcac
     1201 ctgaatacgc tgaggctatc gcaacccgtg caggtgcgct ggctggcatc tctccgatgt
     1261 tccaaccttg cgtagttcct cctaagccgt ggactggcat tactggtggt ggctattggg
     1321 ctaacggtcg tcgtcctctg gcgctggtgc gtactcacag taagaaagca ctgatgcgct
     1381 acgaagacgt ttacatgcct gaggtgtaca aagcgattaa cattgcgcaa aacaccgcat
     1441 ggaaaatcaa caagaaagtc ctagcggtcg ccaacgtaat caccaagtgg aagcattgtc
     1501 cggtcgagga catccctgcg attgagcgtg aagaactccc gatgaaaccg gaagacatcg
     1561 acatgaatcc tgaggctctc accgcgtgga aacgtgctgc cgctgctgtg taccgcaagg
     1621 acaaggctcg caagtctcgc cgtatcagcc ttgagttcat gcttgagcaa gccaataagt
     1681 ttgctaacca taaggccatc tggttccctt acaacatgga ctggcgcggt cgtgtttacg
     1741 ctgtgtcaat gttcaacccg caaggtaacg atatgaccaa aggactgctt acgctggcga
     1801 aaggtaaacc aatcggtaag gaaggttact actggctgaa aatccacggt gcaaactgtg
     1861 cgggtgtcga taaggttccg ttccctgagc gcatcaagtt cattgaggaa aaccacgaga
     1921 acatcatggc ttgcgctaag tctccactgg agaacacttg gtgggctgag caagattctc
     1981 cgttctgctt ccttgcgttc tgctttgagt acgctggggt acagcaccac ggcctgagct
     2041 ataactgctc ccttccgctg gcgtttgacg ggtcttgctc tggcatccag cacttctccg
     2101 cgatgctccg agatgaggta ggtggtcgcg cggttaactt gcttcctagt gaaaccgttc
     2161 aggacatcta cgggattgtt gctaagaaag tcaacgagat tctacaagca gacgcaatca
     2221 atgggaccga taacgaagta gttaccgtga ccgatgagaa cactggtgaa atctctgaga
     2281 aagtcaagct gggcactaag gcactggctg gtcaatggct ggcttacggt gttactcgca
     2341 gtgtgactaa gcgttcagtc atgacgctgg cttacgggtc caaagagttc ggcttccgtc
     2401 aacaagtgct ggaagatacc attcagccag ctattgattc cggcaagggt ctgatgttca
     2461 ctcagccgaa tcaggctgct ggatacatgg ctaagctgat ttgggaatct gtgagcgtga
     2521 cggtggtagc tgcggttgaa gcaatgaact ggcttaagtc tgctgctaag ctgctggctg
     2581 ctgaggtcaa agataagaag actggagaga ttcttcgcaa gcgttgcgct gtgcattggg
     2641 taactcctga tggtttccct gtgtggcagg aatacaagaa gcctattcag acgcgcttga
     2701 acctgatgtt cctcggtcag ttccgcttac agcctaccat taacaccaac aaagatagcg
     2761 agattgatgc acacaaacag gagtctggta tcgctcctaa ctttgtacac agccaagacg
     2821 gtagccacct tcgtaagact gtagtgtggg cacacgagaa gtacggaatc gaatcttttg
     2881 cactgattca cgactccttc ggtaccattc cggctgacgc tgcgaacctg ttcaaagcag
     2941 tgcgcgaaac tatggttgac acatatgagt cttgtgatgt actggctgat ttctacgacc
     3001 agttcgctga ccagttgcac gagtctcaat tggacaaaat gccagcactt ccggctaaag
     3061 gtaacttgaa cctccgtgac atcttagagt cggacttcgc gttcgcataa tactagagcc
     3121 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt
     3181 ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc
     3241 gtttata
//