LOCUS BBa_K1886012 3617 bp DNA linear 22-Dec-2024 DEFINITION AND GATE-input_DPD+green light ACCESSION BBa_K1886012 VERSION BBa_K1886012.1 FEATURES Location/Qualifiers promoter 1..238 /label=PcpcG2 promoter promoter 1..238 /label=BBa_K592003 misc_feature 247..382 /label=BBa_K228001 stem_loop 402..445 /label=stem_loop misc_feature 391..470 /label=BBa_B0010 terminator 391..470 /label=BBa_B0010 stem_loop 486..505 /label=T7 TE misc_feature 512..512 /label=stop polyA_site 506..519 /label=polya misc_feature 479..519 /label=BBa_B0012 terminator 479..519 /label=BBa_B0012 terminator 391..519 /label=BBa_B0015 promoter 528..782 /label=plsr promoter 528..782 /label=BBa_K1666006 RBS 791..801 /label=RBS-4\Weaker RBS 797..800 /label=RBS misc_feature 791..801 /label=BBa_B0033 RBS 791..801 /label=BBa_B0033 CDS 808..3480 /label=cds CDS 808..3480 /label=BBa_K228000 stem_loop 3500..3543 /label=stem_loop misc_feature 3489..3568 /label=BBa_B0010 terminator 3489..3568 /label=BBa_B0010 stem_loop 3584..3603 /label=T7 TE misc_feature 3610..3610 /label=stop polyA_site 3604..3617 /label=polya misc_feature 3577..3617 /label=BBa_B0012 terminator 3577..3617 /label=BBa_B0012 terminator 3489..3617 /label=BBa_B0015 ORIGIN 1 agcccattgt gcttttctct atcaacctca gcttacctga aggggtgaac aggtctgggt 61 taattcatgt tgcgaaatgt aacagtttta gtcgcatcag ctaactttcc gatttcttta 121 cgattttctc ccccttttct tcaattttac tttgttagga tcgcattttt aatgccaaca 181 cataccagtt attggctgga cattaaacaa cttttaagtt taattactaa ctttatctta 241 ctagagcaat tcggagagat gccggagcgg ctgaacggac cggtctctaa aaccggagta 301 ggggcaactc taccgggggt tcaaatcccc ctctctccgc cactacagat ccttagcgaa 361 agctaaggat tttttttaag cttactagag ccaggcatca aataaaacga aaggctcagt 421 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc 481 acactggctc accttcgggt gggcctttct gcgtttatat actagagtgt cataacctgg 541 ctttactttg aacatttcta aatcattaac acaattgttc agttatcact ccgaaataac 601 cgtgattaac gccacaaaaa cgcgccaaat ctgaacattt atcatctaaa aattcattta 661 ttcagaaaac gtgatctgga tgagagtttt ttgaccaaat aactactacc gttttgaaca 721 atttcttttt caaaaaacat ttgttcagtc ccgtcagtca acattgaggg agcggaggca 781 actactagag tcacacagga ctactagatg accatgatta ccgtgcacta gaataccatt 841 aacattgcta agaacgactt ctctgacatc gaactggctg ctatcccgtt caacactctg 901 gctgaccatt acggtgagcg tttagctcgc gaacagttgg cccttgagca tgagtcttac 961 gagatgggtg aagcacgctt ccgcaagatg tttgagcgtc aacttaaagc tggtgaggtt 1021 gcggataacg ctgccgccaa gcctctcatc actaccctac tccctaagat gattgcacgc 1081 atcaacgact ggtttgagga agtgaaagct aagcgcggca agcgcccgac agccttccag 1141 ttcctgtagg aaatcaagcc ggaagccgta gcgtacatca ccattaagac cactctggct 1201 tgcctaacca gtgctgacaa tacaaccgtt caggctgtag caagcgcaat cggtcgggcc 1261 attgaggacg aggctcgctt cggtcgtatc cgtgaccttg aagctaagca cttcaagaaa 1321 aacgttgagg aacaactcaa caagcgcgta gggcacgtct acaagaaagc atttatgcaa 1381 gttgtcgagg ctgacatgct ctctaagggt ctactcggtg gcgaggcgtg gtcttcgtgg 1441 cataaggaag actctattca tgtaggagta cgctgcatcg agatgctcat tgagtcaacc 1501 ggaatggtta gcttacaccg ccaaaatgct ggcgtagtag gtcaagactc tgagactatc 1561 gaactcgcac ctgaatacgc tgaggctatc gcaacccgtg caggtgcgct ggctggcatc 1621 tctccgatgt tccaaccttg cgtagttcct cctaagccgt ggactggcat tactggtggt 1681 ggctattggg ctaacggtcg tcgtcctctg gcgctggtgc gtactcacag taagaaagca 1741 ctgatgcgct acgaagacgt ttacatgcct gaggtgtaca aagcgattaa cattgcgcaa 1801 aacaccgcat ggaaaatcaa caagaaagtc ctagcggtcg ccaacgtaat caccaagtgg 1861 aagcattgtc cggtcgagga catccctgcg attgagcgtg aagaactccc gatgaaaccg 1921 gaagacatcg acatgaatcc tgaggctctc accgcgtgga aacgtgctgc cgctgctgtg 1981 taccgcaagg acaaggctcg caagtctcgc cgtatcagcc ttgagttcat gcttgagcaa 2041 gccaataagt ttgctaacca taaggccatc tggttccctt acaacatgga ctggcgcggt 2101 cgtgtttacg ctgtgtcaat gttcaacccg caaggtaacg atatgaccaa aggactgctt 2161 acgctggcga aaggtaaacc aatcggtaag gaaggttact actggctgaa aatccacggt 2221 gcaaactgtg cgggtgtcga taaggttccg ttccctgagc gcatcaagtt cattgaggaa 2281 aaccacgaga acatcatggc ttgcgctaag tctccactgg agaacacttg gtgggctgag 2341 caagattctc cgttctgctt ccttgcgttc tgctttgagt acgctggggt acagcaccac 2401 ggcctgagct ataactgctc ccttccgctg gcgtttgacg ggtcttgctc tggcatccag 2461 cacttctccg cgatgctccg agatgaggta ggtggtcgcg cggttaactt gcttcctagt 2521 gaaaccgttc aggacatcta cgggattgtt gctaagaaag tcaacgagat tctacaagca 2581 gacgcaatca atgggaccga taacgaagta gttaccgtga ccgatgagaa cactggtgaa 2641 atctctgaga aagtcaagct gggcactaag gcactggctg gtcaatggct ggcttacggt 2701 gttactcgca gtgtgactaa gcgttcagtc atgacgctgg cttacgggtc caaagagttc 2761 ggcttccgtc aacaagtgct ggaagatacc attcagccag ctattgattc cggcaagggt 2821 ctgatgttca ctcagccgaa tcaggctgct ggatacatgg ctaagctgat ttgggaatct 2881 gtgagcgtga cggtggtagc tgcggttgaa gcaatgaact ggcttaagtc tgctgctaag 2941 ctgctggctg ctgaggtcaa agataagaag actggagaga ttcttcgcaa gcgttgcgct 3001 gtgcattggg taactcctga tggtttccct gtgtggcagg aatacaagaa gcctattcag 3061 acgcgcttga acctgatgtt cctcggtcag ttccgcttac agcctaccat taacaccaac 3121 aaagatagcg agattgatgc acacaaacag gagtctggta tcgctcctaa ctttgtacac 3181 agccaagacg gtagccacct tcgtaagact gtagtgtggg cacacgagaa gtacggaatc 3241 gaatcttttg cactgattca cgactccttc ggtaccattc cggctgacgc tgcgaacctg 3301 ttcaaagcag tgcgcgaaac tatggttgac acatatgagt cttgtgatgt actggctgat 3361 ttctacgacc agttcgctga ccagttgcac gagtctcaat tggacaaaat gccagcactt 3421 ccggctaaag gtaacttgaa cctccgtgac atcttagagt cggacttcgc gttcgcataa 3481 tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt 3541 tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg 3601 gcctttctgc gtttata //