BBa_K1886013
type: ComponentDefinition
persistent identity: BBa K1886013
display id: BBa_K1886013
version: 1
was generated by: iGEM to SBOL conversion
description: AND GATE-input_DPD+ red light
created: 2016-10-06T11:00:00Z
modified: 2016-10-19T11:12:23Z
mutable provenance: This part is a combination of DPD input and Red light input. The subparts of this part are from iGEM Distribution Kits.
owned by: james
owned by: myers
mutable description: The circuit to receive input signals is composed of AND GATE, quorum sensing promoter and light-controlled promoter. After being induced by DPD, plsrA promoter activates the expression of T7 RNA polymerase which contains two amber mutations(T7ptag) . The expression of a special tRNA (supD), which is used to identify amber mutation, is activated by Pλ promoter without the restraint of CL protein. In the wild-type E.coli, amber mutation will inhibit the translation of T7 RNA polymerase, and red light could inhibit the produce of CL protein(for more information, see the part"PompC-NOT Gate"). Therefore, when DPD and green light exist together, T7 RNA polymerase will be expressed, and then T7 promoter will be activated to express the downstream gene.
discontinued: false
dominant: false
group u list: _2351_
m user id: 29387
owner id: 29387
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: PT7(BBa_K1321338) contains T7 promoter and RBS(B0034, which enables easy addition to any coding part which does not already have these parts.
star: false
creator: Lejian Jiang
type: DnaRegion
role: SO:0000804
role: partType/Device
component: BBa_K1145005
component: BBa_B0015
component: BBa_K228001
component: BBa_B0033
component: BBa_K1666006
component: BBa_K228000
sequence annotation: BBa_K1145005
sequence annotation: BBa_K228001
sequence annotation: BBa_B0015
sequence annotation: BBa_K1666006
sequence annotation: BBa_B0033
sequence annotation: BBa_K228000
sequence: https://synbiohub.org/public/igem/BBa_K1886013_sequence/1

BBa_K1145005
type: Component
persistent identity: component2521572
display id: component2521572
version: 1
top level: BBa_K1886013
definition: Pλ
access: public

BBa_B0015
type: Component
persistent identity: component2521580
display id: component2521580
version: 1
top level: BBa_K1886013
definition: BBa_B0015
access: public

BBa_K228001
type: Component
persistent identity: component2521573
display id: component2521573
version: 1
top level: BBa_K1886013
definition: BBa_K228001
access: public

BBa_B0033
type: Component
persistent identity: component2521584
display id: component2521584
version: 1
top level: BBa_K1886013
definition: BBa_B0033
access: public

BBa_K1666006
type: Component
persistent identity: component2521582
display id: component2521582
version: 1
top level: BBa_K1886013
definition: BBa_K1666006
access: public

BBa_B0015
type: Component
persistent identity: component2521594
display id: component2521594
version: 1
top level: BBa_K1886013
definition: BBa_B0015
access: public

BBa_K228000
type: Component
persistent identity: component2521587
display id: component2521587
version: 1
top level: BBa_K1886013
definition: BBa_K228000
access: public

BBa_K1145005
type: SequenceAnnotation
persistent identity: annotation2521572
display id: annotation2521572
version: 1
top level: BBa_K1886013
location: https://synbiohub.org/public/igem/BBa_K1886013/annotation2521572/range2521572/1
component: BBa_K1145005

https://synbiohub.org/public/igem/BBa_K1886013/annotation2521572/range2521572/1
type: Range
persistent identity: range2521572
display id: range2521572
version: 1
top level: BBa_K1886013
direction: direction/reverse
start: 1
end: 68
orientation: inline

BBa_K228001
type: SequenceAnnotation
persistent identity: annotation2521573
display id: annotation2521573
version: 1
top level: BBa_K1886013
location: https://synbiohub.org/public/igem/BBa_K1886013/annotation2521573/range2521573/1
component: BBa_K228001

https://synbiohub.org/public/igem/BBa_K1886013/annotation2521573/range2521573/1
type: Range
persistent identity: range2521573
display id: range2521573
version: 1
top level: BBa_K1886013
direction: direction/reverse
start: 77
end: 212
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2521580
display id: annotation2521580
version: 1
top level: BBa_K1886013
location: https://synbiohub.org/public/igem/BBa_K1886013/annotation2521580/range2521580/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1886013/annotation2521580/range2521580/1
type: Range
persistent identity: range2521580
display id: range2521580
version: 1
top level: BBa_K1886013
direction: direction/reverse
start: 221
end: 349
orientation: inline

BBa_K1666006
type: SequenceAnnotation
persistent identity: annotation2521582
display id: annotation2521582
version: 1
top level: BBa_K1886013
location: https://synbiohub.org/public/igem/BBa_K1886013/annotation2521582/range2521582/1
component: BBa_K1666006

https://synbiohub.org/public/igem/BBa_K1886013/annotation2521582/range2521582/1
type: Range
persistent identity: range2521582
display id: range2521582
version: 1
top level: BBa_K1886013
direction: direction/reverse
start: 358
end: 612
orientation: inline

BBa_B0033
type: SequenceAnnotation
persistent identity: annotation2521584
display id: annotation2521584
version: 1
top level: BBa_K1886013
location: https://synbiohub.org/public/igem/BBa_K1886013/annotation2521584/range2521584/1
component: BBa_B0033

https://synbiohub.org/public/igem/BBa_K1886013/annotation2521584/range2521584/1
type: Range
persistent identity: range2521584
display id: range2521584
version: 1
top level: BBa_K1886013
direction: direction/forward
start: 621
end: 631
orientation: inline

BBa_K228000
type: SequenceAnnotation
persistent identity: annotation2521587
display id: annotation2521587
version: 1
top level: BBa_K1886013
location: https://synbiohub.org/public/igem/BBa_K1886013/annotation2521587/range2521587/1
component: BBa_K228000

https://synbiohub.org/public/igem/BBa_K1886013/annotation2521587/range2521587/1
type: Range
persistent identity: range2521587
display id: range2521587
version: 1
top level: BBa_K1886013
direction: direction/reverse
start: 638
end: 3310
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2521594
display id: annotation2521594
version: 1
top level: BBa_K1886013
location: https://synbiohub.org/public/igem/BBa_K1886013/annotation2521594/range2521594/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1886013/annotation2521594/range2521594/1
type: Range
persistent identity: range2521594
display id: range2521594
version: 1
top level: BBa_K1886013
direction: direction/reverse
start: 3319
end: 3447
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_B0033
type: ComponentDefinition
persistent identity: BBa B0033
display id: BBa_B0033
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.4 (weaker) -- derivative of BBa_0030
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Weaker RBS based on Ron Weiss thesis. Strengths relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_44_48_46_1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-3" in figure 4-14 of thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: RBS-4\Weaker
sequence annotation: BBa_B0033
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0033_sequence/1

RBS-4\Weaker
type: SequenceAnnotation
persistent identity: annotation1713
display id: annotation1713
version: 1
top level: BBa_B0033
location: https://synbiohub.org/public/igem/BBa_B0033/annotation1713/range1713/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0033/annotation1713/range1713/1
type: Range
persistent identity: range1713
display id: range1713
version: 1
top level: BBa_B0033
direction: direction/forward
start: 1
end: 11
orientation: inline

BBa_B0033
type: SequenceAnnotation
persistent identity: annotation7028
display id: annotation7028
version: 1
top level: BBa_B0033
location: https://synbiohub.org/public/igem/BBa_B0033/annotation7028/range7028/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0033/annotation7028/range7028/1
type: Range
persistent identity: range7028
display id: range7028
version: 1
top level: BBa_B0033
direction: direction/forward
start: 1
end: 11
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1714
display id: annotation1714
version: 1
top level: BBa_B0033
location: https://synbiohub.org/public/igem/BBa_B0033/annotation1714/range1714/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0033/annotation1714/range1714/1
type: Range
persistent identity: range1714
display id: range1714
version: 1
top level: BBa_B0033
direction: direction/forward
start: 7
end: 10
orientation: inline

BBa_K1666006
type: ComponentDefinition
persistent identity: BBa K1666006
display id: BBa_K1666006
version: 1
was generated by: iGEM to SBOL conversion
description: lsr promoter of LuxS/AI-2 signaling pathway in Salmonalla
created: 2015-09-05T11:00:00Z
modified: 2015-09-18T08:16:38Z
mutable provenance: We extracted the genomic DNA of Salmonella enterica subsp. enterica serovar Typhimurium str. LT2, and used PCR to isolate the promoter plsr.
owned by: james
owned by: myers
mutable description: Quorum sensing is a process of bacterial cell-to-cell communication involving the production and detection of extracellular signaling molecules called autoinducers. And autoinducer-2 (AI-2) has been proposed to serve as a 'universal signal' for interspecies communication. In the LuxS/AI-2 signaling system of Salmonella Typhimurium, AI-2 response involves ATP binding cassette transporter encoded by genes named Lsr (LuxS regulated). And plsr is member of lsr operon which could be repressed by LsrR. In our project, we set this promoter part in front of a blue pigment as a reporter monitoring the presence of AI-2.
discontinued: false
dominant: false
experience: experience/None
group u list: _2084_
m user id: 28478
owner id: 23948
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: The lsrD gene was isolated from the genome of Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 using PCR. We added restriction enzyme sites when designing primers, and the assembly is done through the use of restriction sites (cutting and ligating).
star: false
creator: Heming Wang
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: plsr
sequence: https://synbiohub.org/public/igem/BBa_K1666006_sequence/1

plsr
type: SequenceAnnotation
persistent identity: annotation2445166
display id: annotation2445166
version: 1
top level: BBa_K1666006
location: https://synbiohub.org/public/igem/BBa_K1666006/annotation2445166/range2445166/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_K1666006/annotation2445166/range2445166/1
type: Range
persistent identity: range2445166
display id: range2445166
version: 1
top level: BBa_K1666006
direction: direction/forward
start: 1
end: 255
orientation: inline

Pλ
type: ComponentDefinition
persistent identity: BBa K1145005
display id: BBa_K1145005
version: 1
was generated by: iGEM to SBOL conversion
description: A promoter which can be inhibited by protein CI
created: 2013-09-13T11:00:00Z
modified: 2015-05-08T01:09:26Z
mutable provenance: It comes from the genome of λ phage.
owned by: james
owned by: myers
mutable description: Pλ is a promoter which can be inhibited by protein CI.
discontinued: false
dominant: false
experience: experience/None
group u list: _1457_
m user id: 0
owner id: 16512
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: No.
star: false
creator: KUN LV
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K1145005_sequence/1

BBa_K228000
type: ComponentDefinition
persistent identity: BBa K228000
display id: BBa_K228000
version: 1
was generated by: iGEM to SBOL conversion
description: T7ptag(T7polymerase with amber mutation)
created: 2009-08-24T11:00:00Z
modified: 2015-05-08T01:11:32Z
mutable provenance: The T7ptag is standardlized from the plasmid kindly provided by Voigt Lab.
owned by: james
owned by: myers
mutable description: T7ptag is a coding sequence of T7 polymerase with 2 Amber mutations. The transcription of T7ptag gene can only lead to the generation of its mRNA, further translation into T7 RNA polymerase is blocked because of the amber mutation. Only in the presence of the Amber mutation suppressor BBa_K228001 SupD-tRNA, it can be translated into functional T7 polymerase. T7ptag is a component of the AND Gate constructed by the Voigt Lab(UCSF)
discontinued: false
dominant: false
experience: experience/Works
group u list: _353_
m user id: 0
owner id: 4253
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
star: false
creator: Lin Min
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: cds
sequence: https://synbiohub.org/public/igem/BBa_K228000_sequence/1

cds
type: SequenceAnnotation
persistent identity: annotation2017714
display id: annotation2017714
version: 1
top level: BBa_K228000
location: https://synbiohub.org/public/igem/BBa_K228000/annotation2017714/range2017714/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K228000/annotation2017714/range2017714/1
type: Range
persistent identity: range2017714
display id: range2017714
version: 1
top level: BBa_K228000
direction: direction/forward
start: 1
end: 2673
orientation: inline

BBa_K228001
type: ComponentDefinition
persistent identity: BBa K228001
display id: BBa_K228001
version: 1
was generated by: iGEM to SBOL conversion
description: SupD-tRNA
created: 2009-08-24T11:00:00Z
modified: 2015-05-08T01:11:32Z
mutable provenance: SupD-tRNA again is from Voigt Lab(UCSF), We standardlize this part by PCR strategy.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: The SupD-tRNA part functions as a tRNA, it suppresses the TAG amber stop codon and translate it into a Ser. There is no need to assembly SupD-tRNA with a rbs, since it is not translated. This part, together with the T7ptag part, make an AND Gate, that is, in the presence of both the 2 parts, T7 polymerase is tranlated into a functional polymerase that transcrips a downstream element constructed after a T7 promoter. Which is the output of the AND Gate.
discontinued: false
dominant: false
experience: experience/Works
group u list: _353_
m user id: 0
owner id: 4253
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: There is a PstI site in the original sequence, but luckily it is near the 3' end of this part, so we designed a mutation in our primer and mutated the PstI site.
star: false
creator: Lin Min
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence: https://synbiohub.org/public/igem/BBa_K228001_sequence/1

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

https://synbiohub.org/public/igem/BBa_K1886013_sequence/1
type: Sequence
persistent identity: BBa K1886013 sequence
display id: BBa_K1886013_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggttcctagtaaatatctaacaccgtgcgtgttgactattttacctctggcggtgataatggttgcattactagagcaattcggagagatgccggagcggctgaacggaccggtctctaaaaccggagtaggggcaactctaccgggggttcaaatccccctctctccgccactacagatccttagcgaaagctaaggattttttttaagcttactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtgtcataacctggctttactttgaacatttctaaatcattaacacaattgttcagttatcactccgaaataaccgtgattaacgccacaaaaacgcgccaaatctgaacatttatcatctaaaaattcatttattcagaaaacgtgatctggatgagagttttttgaccaaataactactaccgttttgaacaatttctttttcaaaaaacatttgttcagtcccgtcagtcaacattgagggagcggaggcaactactagagtcacacaggactactagatgaccatgattaccgtgcactagaataccattaacattgctaagaacgacttctctgacatcgaactggctgctatcccgttcaacactctggctgaccattacggtgagcgtttagctcgcgaacagttggcccttgagcatgagtcttacgagatgggtgaagcacgcttccgcaagatgtttgagcgtcaacttaaagctggtgaggttgcggataacgctgccgccaagcctctcatcactaccctactccctaagatgattgcacgcatcaacgactggtttgaggaagtgaaagctaagcgcggcaagcgcccgacagccttccagttcctgtaggaaatcaagccggaagccgtagcgtacatcaccattaagaccactctggcttgcctaaccagtgctgacaatacaaccgttcaggctgtagcaagcgcaatcggtcgggccattgaggacgaggctcgcttcggtcgtatccgtgaccttgaagctaagcacttcaagaaaaacgttgaggaacaactcaacaagcgcgtagggcacgtctacaagaaagcatttatgcaagttgtcgaggctgacatgctctctaagggtctactcggtggcgaggcgtggtcttcgtggcataaggaagactctattcatgtaggagtacgctgcatcgagatgctcattgagtcaaccggaatggttagcttacaccgccaaaatgctggcgtagtaggtcaagactctgagactatcgaactcgcacctgaatacgctgaggctatcgcaacccgtgcaggtgcgctggctggcatctctccgatgttccaaccttgcgtagttcctcctaagccgtggactggcattactggtggtggctattgggctaacggtcgtcgtcctctggcgctggtgcgtactcacagtaagaaagcactgatgcgctacgaagacgtttacatgcctgaggtgtacaaagcgattaacattgcgcaaaacaccgcatggaaaatcaacaagaaagtcctagcggtcgccaacgtaatcaccaagtggaagcattgtccggtcgaggacatccctgcgattgagcgtgaagaactcccgatgaaaccggaagacatcgacatgaatcctgaggctctcaccgcgtggaaacgtgctgccgctgctgtgtaccgcaaggacaaggctcgcaagtctcgccgtatcagccttgagttcatgcttgagcaagccaataagtttgctaaccataaggccatctggttcccttacaacatggactggcgcggtcgtgtttacgctgtgtcaatgttcaacccgcaaggtaacgatatgaccaaaggactgcttacgctggcga…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0033_sequence/1
type: Sequence
persistent identity: BBa B0033 sequence
display id: BBa_B0033_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1666006_sequence/1
type: Sequence
persistent identity: BBa K1666006 sequence
display id: BBa_K1666006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tgtcataacctggctttactttgaacatttctaaatcattaacacaattgttcagttatcactccgaaataaccgtgattaacgccacaaaaacgcgccaaatctgaacatttatcatctaaaaattcatttattcagaaaacgtgatctggatgagagttttttgaccaaataactactaccgttttgaacaatttctttttcaaaaaacatttgttcagtcccgtcagtcaacattgagggagcggaggcaac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1145005_sequence/1
type: Sequence
persistent identity: BBa K1145005 sequence
display id: BBa_K1145005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggttcctagtaaatatctaacaccgtgcgtgttgactattttacctctggcggtgataatggttgcat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K228000_sequence/1
type: Sequence
persistent identity: BBa K228000 sequence
display id: BBa_K228000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaccatgattaccgtgcactagaataccattaacattgctaagaacgacttctctgacatcgaactggctgctatcccgttcaacactctggctgaccattacggtgagcgtttagctcgcgaacagttggcccttgagcatgagtcttacgagatgggtgaagcacgcttccgcaagatgtttgagcgtcaacttaaagctggtgaggttgcggataacgctgccgccaagcctctcatcactaccctactccctaagatgattgcacgcatcaacgactggtttgaggaagtgaaagctaagcgcggcaagcgcccgacagccttccagttcctgtaggaaatcaagccggaagccgtagcgtacatcaccattaagaccactctggcttgcctaaccagtgctgacaatacaaccgttcaggctgtagcaagcgcaatcggtcgggccattgaggacgaggctcgcttcggtcgtatccgtgaccttgaagctaagcacttcaagaaaaacgttgaggaacaactcaacaagcgcgtagggcacgtctacaagaaagcatttatgcaagttgtcgaggctgacatgctctctaagggtctactcggtggcgaggcgtggtcttcgtggcataaggaagactctattcatgtaggagtacgctgcatcgagatgctcattgagtcaaccggaatggttagcttacaccgccaaaatgctggcgtagtaggtcaagactctgagactatcgaactcgcacctgaatacgctgaggctatcgcaacccgtgcaggtgcgctggctggcatctctccgatgttccaaccttgcgtagttcctcctaagccgtggactggcattactggtggtggctattgggctaacggtcgtcgtcctctggcgctggtgcgtactcacagtaagaaagcactgatgcgctacgaagacgtttacatgcctgaggtgtacaaagcgattaacattgcgcaaaacaccgcatggaaaatcaacaagaaagtcctagcggtcgccaacgtaatcaccaagtggaagcattgtccggtcgaggacatccctgcgattgagcgtgaagaactcccgatgaaaccggaagacatcgacatgaatcctgaggctctcaccgcgtggaaacgtgctgccgctgctgtgtaccgcaaggacaaggctcgcaagtctcgccgtatcagccttgagttcatgcttgagcaagccaataagtttgctaaccataaggccatctggttcccttacaacatggactggcgcggtcgtgtttacgctgtgtcaatgttcaacccgcaaggtaacgatatgaccaaaggactgcttacgctggcgaaaggtaaaccaatcggtaaggaaggttactactggctgaaaatccacggtgcaaactgtgcgggtgtcgataaggttccgttccctgagcgcatcaagttcattgaggaaaaccacgagaacatcatggcttgcgctaagtctccactggagaacacttggtgggctgagcaagattctccgttctgcttccttgcgttctgctttgagtacgctggggtacagcaccacggcctgagctataactgctcccttccgctggcgtttgacgggtcttgctctggcatccagcacttctccgcgatgctccgagatgaggtaggtggtcgcgcggttaacttgcttcctagtgaaaccgttcaggacatctacgggattgttgctaagaaagtcaacgagattctacaagcagacgcaatcaatgggaccgataacgaagtagttaccgtgaccgatgagaacactggtgaaatctctgagaaagtcaagctgggcactaaggcactggctggtcaatggctggcttacggtgttactcgcagtgtgactaagcgttcagtcatgacgctggcttacgggtccaaagagttcggcttccgtcaacaagtgctggaagataccattcagccagctattga…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K228001_sequence/1
type: Sequence
persistent identity: BBa K228001 sequence
display id: BBa_K228001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caattcggagagatgccggagcggctgaacggaccggtctctaaaaccggagtaggggcaactctaccgggggttcaaatccccctctctccgccactacagatccttagcgaaagctaaggattttttttaagct
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z