LOCUS BBa_K1886014 3392 bp DNA linear 16-Feb-2025 DEFINITION AND GATE-input_red light+IPTG ACCESSION BBa_K1886014 VERSION BBa_K1886014.1 FEATURES Location/Qualifiers promoter 1..68 /label=BBa_K1145005 misc_feature 77..212 /label=BBa_K228001 stem_loop 232..275 /label=stem_loop misc_feature 221..300 /label=BBa_B0010 terminator 221..300 /label=BBa_B0010 stem_loop 316..335 /label=T7 TE misc_feature 342..342 /label=stop polyA_site 336..349 /label=polya misc_feature 309..349 /label=BBa_B0012 terminator 309..349 /label=BBa_B0012 terminator 221..349 /label=BBa_B0015 CDS 358..445 /label=end of LacI coding region (inactive) misc_feature 358..557 /label=BBa_R0010 misc_feature 446..483 /label=CAP binding site promoter 494..499 /label=-35 promoter 518..523 /label=-10 misc_feature 523..557 /label=LacI binding site misc_feature 530..530 /label=start promoter 358..557 /label=BBa_R0010 RBS 566..576 /label=RBS-4\Weaker RBS 572..575 /label=RBS misc_feature 566..576 /label=BBa_B0033 RBS 566..576 /label=BBa_B0033 CDS 583..3255 /label=cds CDS 583..3255 /label=BBa_K228000 stem_loop 3275..3318 /label=stem_loop misc_feature 3264..3343 /label=BBa_B0010 terminator 3264..3343 /label=BBa_B0010 stem_loop 3359..3378 /label=T7 TE misc_feature 3385..3385 /label=stop polyA_site 3379..3392 /label=polya misc_feature 3352..3392 /label=BBa_B0012 terminator 3352..3392 /label=BBa_B0012 terminator 3264..3392 /label=BBa_B0015 ORIGIN 1 ggttcctagt aaatatctaa caccgtgcgt gttgactatt ttacctctgg cggtgataat 61 ggttgcatta ctagagcaat tcggagagat gccggagcgg ctgaacggac cggtctctaa 121 aaccggagta ggggcaactc taccgggggt tcaaatcccc ctctctccgc cactacagat 181 ccttagcgaa agctaaggat tttttttaag cttactagag ccaggcatca aataaaacga 241 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc 301 tactagagtc acactggctc accttcgggt gggcctttct gcgtttatat actagagcaa 361 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt 421 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt 481 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg 541 gataacaatt tcacacatac tagagtcaca caggactact agatgaccat gattaccgtg 601 cactagaata ccattaacat tgctaagaac gacttctctg acatcgaact ggctgctatc 661 ccgttcaaca ctctggctga ccattacggt gagcgtttag ctcgcgaaca gttggccctt 721 gagcatgagt cttacgagat gggtgaagca cgcttccgca agatgtttga gcgtcaactt 781 aaagctggtg aggttgcgga taacgctgcc gccaagcctc tcatcactac cctactccct 841 aagatgattg cacgcatcaa cgactggttt gaggaagtga aagctaagcg cggcaagcgc 901 ccgacagcct tccagttcct gtaggaaatc aagccggaag ccgtagcgta catcaccatt 961 aagaccactc tggcttgcct aaccagtgct gacaatacaa ccgttcaggc tgtagcaagc 1021 gcaatcggtc gggccattga ggacgaggct cgcttcggtc gtatccgtga ccttgaagct 1081 aagcacttca agaaaaacgt tgaggaacaa ctcaacaagc gcgtagggca cgtctacaag 1141 aaagcattta tgcaagttgt cgaggctgac atgctctcta agggtctact cggtggcgag 1201 gcgtggtctt cgtggcataa ggaagactct attcatgtag gagtacgctg catcgagatg 1261 ctcattgagt caaccggaat ggttagctta caccgccaaa atgctggcgt agtaggtcaa 1321 gactctgaga ctatcgaact cgcacctgaa tacgctgagg ctatcgcaac ccgtgcaggt 1381 gcgctggctg gcatctctcc gatgttccaa ccttgcgtag ttcctcctaa gccgtggact 1441 ggcattactg gtggtggcta ttgggctaac ggtcgtcgtc ctctggcgct ggtgcgtact 1501 cacagtaaga aagcactgat gcgctacgaa gacgtttaca tgcctgaggt gtacaaagcg 1561 attaacattg cgcaaaacac cgcatggaaa atcaacaaga aagtcctagc ggtcgccaac 1621 gtaatcacca agtggaagca ttgtccggtc gaggacatcc ctgcgattga gcgtgaagaa 1681 ctcccgatga aaccggaaga catcgacatg aatcctgagg ctctcaccgc gtggaaacgt 1741 gctgccgctg ctgtgtaccg caaggacaag gctcgcaagt ctcgccgtat cagccttgag 1801 ttcatgcttg agcaagccaa taagtttgct aaccataagg ccatctggtt cccttacaac 1861 atggactggc gcggtcgtgt ttacgctgtg tcaatgttca acccgcaagg taacgatatg 1921 accaaaggac tgcttacgct ggcgaaaggt aaaccaatcg gtaaggaagg ttactactgg 1981 ctgaaaatcc acggtgcaaa ctgtgcgggt gtcgataagg ttccgttccc tgagcgcatc 2041 aagttcattg aggaaaacca cgagaacatc atggcttgcg ctaagtctcc actggagaac 2101 acttggtggg ctgagcaaga ttctccgttc tgcttccttg cgttctgctt tgagtacgct 2161 ggggtacagc accacggcct gagctataac tgctcccttc cgctggcgtt tgacgggtct 2221 tgctctggca tccagcactt ctccgcgatg ctccgagatg aggtaggtgg tcgcgcggtt 2281 aacttgcttc ctagtgaaac cgttcaggac atctacggga ttgttgctaa gaaagtcaac 2341 gagattctac aagcagacgc aatcaatggg accgataacg aagtagttac cgtgaccgat 2401 gagaacactg gtgaaatctc tgagaaagtc aagctgggca ctaaggcact ggctggtcaa 2461 tggctggctt acggtgttac tcgcagtgtg actaagcgtt cagtcatgac gctggcttac 2521 gggtccaaag agttcggctt ccgtcaacaa gtgctggaag ataccattca gccagctatt 2581 gattccggca agggtctgat gttcactcag ccgaatcagg ctgctggata catggctaag 2641 ctgatttggg aatctgtgag cgtgacggtg gtagctgcgg ttgaagcaat gaactggctt 2701 aagtctgctg ctaagctgct ggctgctgag gtcaaagata agaagactgg agagattctt 2761 cgcaagcgtt gcgctgtgca ttgggtaact cctgatggtt tccctgtgtg gcaggaatac 2821 aagaagccta ttcagacgcg cttgaacctg atgttcctcg gtcagttccg cttacagcct 2881 accattaaca ccaacaaaga tagcgagatt gatgcacaca aacaggagtc tggtatcgct 2941 cctaactttg tacacagcca agacggtagc caccttcgta agactgtagt gtgggcacac 3001 gagaagtacg gaatcgaatc ttttgcactg attcacgact ccttcggtac cattccggct 3061 gacgctgcga acctgttcaa agcagtgcgc gaaactatgg ttgacacata tgagtcttgt 3121 gatgtactgg ctgatttcta cgaccagttc gctgaccagt tgcacgagtc tcaattggac 3181 aaaatgccag cacttccggc taaaggtaac ttgaacctcc gtgacatctt agagtcggac 3241 ttcgcgttcg cataatacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga 3301 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg 3361 ctcaccttcg ggtgggcctt tctgcgttta ta //