##gff-version 3
##sequence-region BBa_K1886014 1 3392
BBa_K1886014	.	terminator	221	349	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1886014	.	terminator	221	300	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1886014	.	stem_loop	232	275	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1886014	.	engineered_region	221	300	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1886014	.	terminator	309	349	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1886014	.	engineered_region	309	349	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1886014	.	stop_codon	342	342	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1886014	.	stem_loop	316	335	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1886014	.	polyA_site	336	349	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1886014	.	ribosome_entry_site	566	576	.	+	0	ID=BBa_B0033;Name=BBa_B0033
BBa_K1886014	.	ribosome_entry_site	572	575	.	+	0	ID=annotation1714;Name=RBS;Parent=BBa_B0033
BBa_K1886014	.	ribosome_entry_site	566	576	.	+	0	ID=annotation1713;Name=RBS-4\Weaker;Parent=BBa_B0033
BBa_K1886014	.	engineered_region	566	576	.	+	0	ID=annotation7028;Name=BBa_B0033;Parent=BBa_B0033
BBa_K1886014	.	promoter	358	557	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_K1886014	.	non_covalent_binding_site	523	557	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K1886014	.	non_covalent_binding_site	446	483	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K1886014	.	engineered_region	358	557	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K1886014	.	promoter	494	499	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K1886014	.	CDS	358	445	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K1886014	.	start_codon	530	530	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K1886014	.	promoter	518	523	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K1886014	.	mature_transcript_region	77	212	.	+	0	ID=BBa_K228001;Name=BBa_K228001
BBa_K1886014	.	CDS	583	3255	.	+	0	ID=BBa_K228000;Name=BBa_K228000
BBa_K1886014	.	CDS	583	3255	.	+	0	ID=annotation2017714;Name=cds;Parent=BBa_K228000
BBa_K1886014	.	terminator	3264	3392	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1886014	.	terminator	3264	3343	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1886014	.	stem_loop	3275	3318	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1886014	.	engineered_region	3264	3343	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1886014	.	terminator	3352	3392	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1886014	.	engineered_region	3352	3392	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1886014	.	stop_codon	3385	3385	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1886014	.	stem_loop	3359	3378	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1886014	.	polyA_site	3379	3392	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1886014	.	promoter	1	68	.	+	0	ID=BBa_K1145005;Name=BBa_K1145005
>BBa_K1886014
ggttcctagtaaatatctaacaccgtgcgtgttgactattttacctctggcggtgataatggttgcattactagagcaa
ttcggagagatgccggagcggctgaacggaccggtctctaaaaccggagtaggggcaactctaccgggggttcaaatcc
ccctctctccgccactacagatccttagcgaaagctaaggattttttttaagcttactagagccaggcatcaaataaaa
cgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactg
gctcaccttcgggtgggcctttctgcgtttatatactagagcaatacgcaaaccgcctctccccgcgcgttggccgatt
cattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctca
ctcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttca
cacatactagagtcacacaggactactagatgaccatgattaccgtgcactagaataccattaacattgctaagaacga
cttctctgacatcgaactggctgctatcccgttcaacactctggctgaccattacggtgagcgtttagctcgcgaacag
ttggcccttgagcatgagtcttacgagatgggtgaagcacgcttccgcaagatgtttgagcgtcaacttaaagctggtg
aggttgcggataacgctgccgccaagcctctcatcactaccctactccctaagatgattgcacgcatcaacgactggtt
tgaggaagtgaaagctaagcgcggcaagcgcccgacagccttccagttcctgtaggaaatcaagccggaagccgtagcg
tacatcaccattaagaccactctggcttgcctaaccagtgctgacaatacaaccgttcaggctgtagcaagcgcaatcg
gtcgggccattgaggacgaggctcgcttcggtcgtatccgtgaccttgaagctaagcacttcaagaaaaacgttgagga
acaactcaacaagcgcgtagggcacgtctacaagaaagcatttatgcaagttgtcgaggctgacatgctctctaagggt
ctactcggtggcgaggcgtggtcttcgtggcataaggaagactctattcatgtaggagtacgctgcatcgagatgctca
ttgagtcaaccggaatggttagcttacaccgccaaaatgctggcgtagtaggtcaagactctgagactatcgaactcgc
acctgaatacgctgaggctatcgcaacccgtgcaggtgcgctggctggcatctctccgatgttccaaccttgcgtagtt
cctcctaagccgtggactggcattactggtggtggctattgggctaacggtcgtcgtcctctggcgctggtgcgtactc
acagtaagaaagcactgatgcgctacgaagacgtttacatgcctgaggtgtacaaagcgattaacattgcgcaaaacac
cgcatggaaaatcaacaagaaagtcctagcggtcgccaacgtaatcaccaagtggaagcattgtccggtcgaggacatc
cctgcgattgagcgtgaagaactcccgatgaaaccggaagacatcgacatgaatcctgaggctctcaccgcgtggaaac
gtgctgccgctgctgtgtaccgcaaggacaaggctcgcaagtctcgccgtatcagccttgagttcatgcttgagcaagc
caataagtttgctaaccataaggccatctggttcccttacaacatggactggcgcggtcgtgtttacgctgtgtcaatg
ttcaacccgcaaggtaacgatatgaccaaaggactgcttacgctggcgaaaggtaaaccaatcggtaaggaaggttact
actggctgaaaatccacggtgcaaactgtgcgggtgtcgataaggttccgttccctgagcgcatcaagttcattgagga
aaaccacgagaacatcatggcttgcgctaagtctccactggagaacacttggtgggctgagcaagattctccgttctgc
ttccttgcgttctgctttgagtacgctggggtacagcaccacggcctgagctataactgctcccttccgctggcgtttg
acgggtcttgctctggcatccagcacttctccgcgatgctccgagatgaggtaggtggtcgcgcggttaacttgcttcc
tagtgaaaccgttcaggacatctacgggattgttgctaagaaagtcaacgagattctacaagcagacgcaatcaatggg
accgataacgaagtagttaccgtgaccgatgagaacactggtgaaatctctgagaaagtcaagctgggcactaaggcac
tggctggtcaatggctggcttacggtgttactcgcagtgtgactaagcgttcagtcatgacgctggcttacgggtccaa
agagttcggcttccgtcaacaagtgctggaagataccattcagccagctattgattccggcaagggtctgatgttcact
cagccgaatcaggctgctggatacatggctaagctgatttgggaatctgtgagcgtgacggtggtagctgcggttgaag
caatgaactggcttaagtctgctgctaagctgctggctgctgaggtcaaagataagaagactggagagattcttcgcaa
gcgttgcgctgtgcattgggtaactcctgatggtttccctgtgtggcaggaatacaagaagcctattcagacgcgcttg
aacctgatgttcctcggtcagttccgcttacagcctaccattaacaccaacaaagatagcgagattgatgcacacaaac
aggagtctggtatcgctcctaactttgtacacagccaagacggtagccaccttcgtaagactgtagtgtgggcacacga
gaagtacggaatcgaatcttttgcactgattcacgactccttcggtaccattccggctgacgctgcgaacctgttcaaa
gcagtgcgcgaaactatggttgacacatatgagtcttgtgatgtactggctgatttctacgaccagttcgctgaccagt
tgcacgagtctcaattggacaaaatgccagcacttccggctaaaggtaacttgaacctccgtgacatcttagagtcgga
cttcgcgttcgcataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttat
ctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata