##gff-version 3
##sequence-region BBa_K1886015 1 3562
BBa_K1886015	.	terminator	3434	3562	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1886015	.	terminator	3522	3562	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1886015	.	polyA_site	3549	3562	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1886015	.	engineered_region	3522	3562	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1886015	.	stem_loop	3529	3548	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1886015	.	stop_codon	3555	3555	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1886015	.	terminator	3434	3513	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1886015	.	stem_loop	3445	3488	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1886015	.	engineered_region	3434	3513	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1886015	.	terminator	391	519	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1886015	.	terminator	479	519	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1886015	.	polyA_site	506	519	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1886015	.	engineered_region	479	519	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1886015	.	stem_loop	486	505	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1886015	.	stop_codon	512	512	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1886015	.	terminator	391	470	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1886015	.	stem_loop	402	445	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1886015	.	engineered_region	391	470	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1886015	.	ribosome_entry_site	736	746	.	+	0	ID=BBa_B0033;Name=BBa_B0033
BBa_K1886015	.	ribosome_entry_site	736	746	.	+	0	ID=annotation1713;Name=RBS-4\Weaker;Parent=BBa_B0033
BBa_K1886015	.	engineered_region	736	746	.	+	0	ID=annotation7028;Name=BBa_B0033;Parent=BBa_B0033
BBa_K1886015	.	ribosome_entry_site	742	745	.	+	0	ID=annotation1714;Name=RBS;Parent=BBa_B0033
BBa_K1886015	.	CDS	753	3425	.	+	0	ID=BBa_K228000;Name=BBa_K228000
BBa_K1886015	.	CDS	753	3425	.	+	0	ID=annotation2017714;Name=cds;Parent=BBa_K228000
BBa_K1886015	.	mature_transcript_region	247	382	.	+	0	ID=BBa_K228001;Name=BBa_K228001
BBa_K1886015	.	promoter	528	727	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_K1886015	.	start_codon	700	700	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K1886015	.	CDS	528	615	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K1886015	.	promoter	688	693	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K1886015	.	non_covalent_binding_site	693	727	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K1886015	.	non_covalent_binding_site	616	653	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K1886015	.	engineered_region	528	727	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K1886015	.	promoter	664	669	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K1886015	.	promoter	1	238	.	+	0	ID=BBa_K592003;Name=BBa_K592003
BBa_K1886015	.	promoter	1	238	.	+	0	ID=annotation2130109;Name=PcpcG2 promoter;Parent=BBa_K592003
>BBa_K1886015
agcccattgtgcttttctctatcaacctcagcttacctgaaggggtgaacaggtctgggttaattcatgttgcgaaatg
taacagttttagtcgcatcagctaactttccgatttctttacgattttctcccccttttcttcaattttactttgttag
gatcgcatttttaatgccaacacataccagttattggctggacattaaacaacttttaagtttaattactaactttatc
ttactagagcaattcggagagatgccggagcggctgaacggaccggtctctaaaaccggagtaggggcaactctaccgg
gggttcaaatccccctctctccgccactacagatccttagcgaaagctaaggattttttttaagcttactagagccagg
catcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctact
agagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagcaatacgcaaaccgcctctccccgcg
cgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatg
tgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcgg
ataacaatttcacacatactagagtcacacaggactactagatgaccatgattaccgtgcactagaataccattaacat
tgctaagaacgacttctctgacatcgaactggctgctatcccgttcaacactctggctgaccattacggtgagcgttta
gctcgcgaacagttggcccttgagcatgagtcttacgagatgggtgaagcacgcttccgcaagatgtttgagcgtcaac
ttaaagctggtgaggttgcggataacgctgccgccaagcctctcatcactaccctactccctaagatgattgcacgcat
caacgactggtttgaggaagtgaaagctaagcgcggcaagcgcccgacagccttccagttcctgtaggaaatcaagccg
gaagccgtagcgtacatcaccattaagaccactctggcttgcctaaccagtgctgacaatacaaccgttcaggctgtag
caagcgcaatcggtcgggccattgaggacgaggctcgcttcggtcgtatccgtgaccttgaagctaagcacttcaagaa
aaacgttgaggaacaactcaacaagcgcgtagggcacgtctacaagaaagcatttatgcaagttgtcgaggctgacatg
ctctctaagggtctactcggtggcgaggcgtggtcttcgtggcataaggaagactctattcatgtaggagtacgctgca
tcgagatgctcattgagtcaaccggaatggttagcttacaccgccaaaatgctggcgtagtaggtcaagactctgagac
tatcgaactcgcacctgaatacgctgaggctatcgcaacccgtgcaggtgcgctggctggcatctctccgatgttccaa
ccttgcgtagttcctcctaagccgtggactggcattactggtggtggctattgggctaacggtcgtcgtcctctggcgc
tggtgcgtactcacagtaagaaagcactgatgcgctacgaagacgtttacatgcctgaggtgtacaaagcgattaacat
tgcgcaaaacaccgcatggaaaatcaacaagaaagtcctagcggtcgccaacgtaatcaccaagtggaagcattgtccg
gtcgaggacatccctgcgattgagcgtgaagaactcccgatgaaaccggaagacatcgacatgaatcctgaggctctca
ccgcgtggaaacgtgctgccgctgctgtgtaccgcaaggacaaggctcgcaagtctcgccgtatcagccttgagttcat
gcttgagcaagccaataagtttgctaaccataaggccatctggttcccttacaacatggactggcgcggtcgtgtttac
gctgtgtcaatgttcaacccgcaaggtaacgatatgaccaaaggactgcttacgctggcgaaaggtaaaccaatcggta
aggaaggttactactggctgaaaatccacggtgcaaactgtgcgggtgtcgataaggttccgttccctgagcgcatcaa
gttcattgaggaaaaccacgagaacatcatggcttgcgctaagtctccactggagaacacttggtgggctgagcaagat
tctccgttctgcttccttgcgttctgctttgagtacgctggggtacagcaccacggcctgagctataactgctcccttc
cgctggcgtttgacgggtcttgctctggcatccagcacttctccgcgatgctccgagatgaggtaggtggtcgcgcggt
taacttgcttcctagtgaaaccgttcaggacatctacgggattgttgctaagaaagtcaacgagattctacaagcagac
gcaatcaatgggaccgataacgaagtagttaccgtgaccgatgagaacactggtgaaatctctgagaaagtcaagctgg
gcactaaggcactggctggtcaatggctggcttacggtgttactcgcagtgtgactaagcgttcagtcatgacgctggc
ttacgggtccaaagagttcggcttccgtcaacaagtgctggaagataccattcagccagctattgattccggcaagggt
ctgatgttcactcagccgaatcaggctgctggatacatggctaagctgatttgggaatctgtgagcgtgacggtggtag
ctgcggttgaagcaatgaactggcttaagtctgctgctaagctgctggctgctgaggtcaaagataagaagactggaga
gattcttcgcaagcgttgcgctgtgcattgggtaactcctgatggtttccctgtgtggcaggaatacaagaagcctatt
cagacgcgcttgaacctgatgttcctcggtcagttccgcttacagcctaccattaacaccaacaaagatagcgagattg
atgcacacaaacaggagtctggtatcgctcctaactttgtacacagccaagacggtagccaccttcgtaagactgtagt
gtgggcacacgagaagtacggaatcgaatcttttgcactgattcacgactccttcggtaccattccggctgacgctgcg
aacctgttcaaagcagtgcgcgaaactatggttgacacatatgagtcttgtgatgtactggctgatttctacgaccagt
tcgctgaccagttgcacgagtctcaattggacaaaatgccagcacttccggctaaaggtaacttgaacctccgtgacat
cttagagtcggacttcgcgttcgcataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggc
ctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgc
gtttata