LOCUS BBa_K1886016 4368 bp DNA linear 22-Nov-2024 DEFINITION AND GATE-input_ara+IPTG ACCESSION BBa_K1886016 VERSION BBa_K1886016.1 FEATURES Location/Qualifiers promoter 1..130 /label=BBa_I13453 misc_feature 139..274 /label=BBa_K228001 stem_loop 294..337 /label=stem_loop misc_feature 283..362 /label=BBa_B0010 terminator 283..362 /label=BBa_B0010 stem_loop 378..397 /label=T7 TE misc_feature 404..404 /label=stop polyA_site 398..411 /label=polya misc_feature 371..411 /label=BBa_B0012 terminator 371..411 /label=BBa_B0012 terminator 283..411 /label=BBa_B0015 CDS 420..507 /label=end of LacI coding region (inactive) misc_feature 420..619 /label=BBa_R0010 misc_feature 508..545 /label=CAP binding site promoter 556..561 /label=-35 promoter 580..585 /label=-10 misc_feature 585..619 /label=LacI binding site misc_feature 592..592 /label=start promoter 420..619 /label=BBa_R0010 RBS 628..638 /label=RBS-4\Weaker RBS 634..637 /label=RBS misc_feature 628..638 /label=BBa_B0033 RBS 628..638 /label=BBa_B0033 CDS 645..3317 /label=cds CDS 645..3317 /label=BBa_K228000 stem_loop 3337..3380 /label=stem_loop misc_feature 3326..3405 /label=BBa_B0010 terminator 3326..3405 /label=BBa_B0010 stem_loop 3421..3440 /label=T7 TE misc_feature 3447..3447 /label=stop polyA_site 3441..3454 /label=polya misc_feature 3414..3454 /label=BBa_B0012 terminator 3414..3454 /label=BBa_B0012 terminator 3326..3454 /label=BBa_B0015 promoter 3463..3485 /label=BBa_I719005 misc_feature 3498..3501 /label=conserved RBS 3494..3505 /label=BBa_B0034 CDS 3463..3505 /label=BBa_K1321338 CDS 3512..4231 /label=GFP misc_feature 3512..4231 /label=BBa_K895006 stem_loop 4251..4294 /label=stem_loop misc_feature 4240..4319 /label=BBa_B0010 terminator 4240..4319 /label=BBa_B0010 stem_loop 4335..4354 /label=T7 TE misc_feature 4361..4361 /label=stop polyA_site 4355..4368 /label=polya misc_feature 4328..4368 /label=BBa_B0012 terminator 4328..4368 /label=BBa_B0012 terminator 4240..4368 /label=BBa_B0015 ORIGIN 1 acattgatta tttgcacggc gtcacacttt gctatgccat agcattttta tccataagat 61 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca taccgttttt 121 ttgggctagc tactagagca attcggagag atgccggagc ggctgaacgg accggtctct 181 aaaaccggag taggggcaac tctaccgggg gttcaaatcc ccctctctcc gccactacag 241 atccttagcg aaagctaagg atttttttta agcttactag agccaggcat caaataaaac 301 gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc 361 tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat atactagagc 421 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag 481 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca 541 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag 601 cggataacaa tttcacacat actagagtca cacaggacta ctagatgacc atgattaccg 661 tgcactagaa taccattaac attgctaaga acgacttctc tgacatcgaa ctggctgcta 721 tcccgttcaa cactctggct gaccattacg gtgagcgttt agctcgcgaa cagttggccc 781 ttgagcatga gtcttacgag atgggtgaag cacgcttccg caagatgttt gagcgtcaac 841 ttaaagctgg tgaggttgcg gataacgctg ccgccaagcc tctcatcact accctactcc 901 ctaagatgat tgcacgcatc aacgactggt ttgaggaagt gaaagctaag cgcggcaagc 961 gcccgacagc cttccagttc ctgtaggaaa tcaagccgga agccgtagcg tacatcacca 1021 ttaagaccac tctggcttgc ctaaccagtg ctgacaatac aaccgttcag gctgtagcaa 1081 gcgcaatcgg tcgggccatt gaggacgagg ctcgcttcgg tcgtatccgt gaccttgaag 1141 ctaagcactt caagaaaaac gttgaggaac aactcaacaa gcgcgtaggg cacgtctaca 1201 agaaagcatt tatgcaagtt gtcgaggctg acatgctctc taagggtcta ctcggtggcg 1261 aggcgtggtc ttcgtggcat aaggaagact ctattcatgt aggagtacgc tgcatcgaga 1321 tgctcattga gtcaaccgga atggttagct tacaccgcca aaatgctggc gtagtaggtc 1381 aagactctga gactatcgaa ctcgcacctg aatacgctga ggctatcgca acccgtgcag 1441 gtgcgctggc tggcatctct ccgatgttcc aaccttgcgt agttcctcct aagccgtgga 1501 ctggcattac tggtggtggc tattgggcta acggtcgtcg tcctctggcg ctggtgcgta 1561 ctcacagtaa gaaagcactg atgcgctacg aagacgttta catgcctgag gtgtacaaag 1621 cgattaacat tgcgcaaaac accgcatgga aaatcaacaa gaaagtccta gcggtcgcca 1681 acgtaatcac caagtggaag cattgtccgg tcgaggacat ccctgcgatt gagcgtgaag 1741 aactcccgat gaaaccggaa gacatcgaca tgaatcctga ggctctcacc gcgtggaaac 1801 gtgctgccgc tgctgtgtac cgcaaggaca aggctcgcaa gtctcgccgt atcagccttg 1861 agttcatgct tgagcaagcc aataagtttg ctaaccataa ggccatctgg ttcccttaca 1921 acatggactg gcgcggtcgt gtttacgctg tgtcaatgtt caacccgcaa ggtaacgata 1981 tgaccaaagg actgcttacg ctggcgaaag gtaaaccaat cggtaaggaa ggttactact 2041 ggctgaaaat ccacggtgca aactgtgcgg gtgtcgataa ggttccgttc cctgagcgca 2101 tcaagttcat tgaggaaaac cacgagaaca tcatggcttg cgctaagtct ccactggaga 2161 acacttggtg ggctgagcaa gattctccgt tctgcttcct tgcgttctgc tttgagtacg 2221 ctggggtaca gcaccacggc ctgagctata actgctccct tccgctggcg tttgacgggt 2281 cttgctctgg catccagcac ttctccgcga tgctccgaga tgaggtaggt ggtcgcgcgg 2341 ttaacttgct tcctagtgaa accgttcagg acatctacgg gattgttgct aagaaagtca 2401 acgagattct acaagcagac gcaatcaatg ggaccgataa cgaagtagtt accgtgaccg 2461 atgagaacac tggtgaaatc tctgagaaag tcaagctggg cactaaggca ctggctggtc 2521 aatggctggc ttacggtgtt actcgcagtg tgactaagcg ttcagtcatg acgctggctt 2581 acgggtccaa agagttcggc ttccgtcaac aagtgctgga agataccatt cagccagcta 2641 ttgattccgg caagggtctg atgttcactc agccgaatca ggctgctgga tacatggcta 2701 agctgatttg ggaatctgtg agcgtgacgg tggtagctgc ggttgaagca atgaactggc 2761 ttaagtctgc tgctaagctg ctggctgctg aggtcaaaga taagaagact ggagagattc 2821 ttcgcaagcg ttgcgctgtg cattgggtaa ctcctgatgg tttccctgtg tggcaggaat 2881 acaagaagcc tattcagacg cgcttgaacc tgatgttcct cggtcagttc cgcttacagc 2941 ctaccattaa caccaacaaa gatagcgaga ttgatgcaca caaacaggag tctggtatcg 3001 ctcctaactt tgtacacagc caagacggta gccaccttcg taagactgta gtgtgggcac 3061 acgagaagta cggaatcgaa tcttttgcac tgattcacga ctccttcggt accattccgg 3121 ctgacgctgc gaacctgttc aaagcagtgc gcgaaactat ggttgacaca tatgagtctt 3181 gtgatgtact ggctgatttc tacgaccagt tcgctgacca gttgcacgag tctcaattgg 3241 acaaaatgcc agcacttccg gctaaaggta acttgaacct ccgtgacatc ttagagtcgg 3301 acttcgcgtt cgcataatac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa 3361 gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact 3421 ggctcacctt cgggtgggcc tttctgcgtt tatatactag agtaatacga ctcactatag 3481 ggagatacta gagaaagagg agaaatacta gatgcgtaaa ggagaagaac ttttcactgg 3541 agttgtccca attcttgttg aattagatgg tgatgttaat gggcacaaat tttctgtcag 3601 tggagagggt gaaggtgatg caacatacgg aaaacttacc cttaaattta tttgcactac 3661 tggaaaacta cctgttccat ggccaacact tgtcactact ttcggttatg gtgttcaatg 3721 ctttgcgaga tacccagatc atatgaaaca gcatgacttt ttcaagagtg ccatgcccga 3781 aggttatgta caggaaagaa ctatattttt caaagatgac gggaactaca agacacgtgc 3841 tgaagtcaag tttgaaggtg atacccttgt taatagaatc gagttaaaag gtattgattt 3901 taaagaagat ggaaacattc ttggacacaa attggaatac aactataact cacacaatgt 3961 atacatcatg gcagacaaac aaaagaatgg aatcaaagtt aacttcaaaa ttagacacaa 4021 cattgaagat ggaagcgttc aactagcaga ccattatcaa caaaatactc caattggcga 4081 tggccctgtc cttttaccag acaaccatta cctgtccaca caatctgccc tttcgaaaga 4141 tcccaacgaa aagagagacc acatggtcct tcttgagttt gtaacagctg ctgggattac 4201 acatggcatg gatgaactat acaaataata atactagagc caggcatcaa ataaaacgaa 4261 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct 4321 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttata //