##gff-version 3
##sequence-region BBa_K1886016 1 4368
BBa_K1886016	.	terminator	283	411	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1886016	.	terminator	283	362	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1886016	.	engineered_region	283	362	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1886016	.	stem_loop	294	337	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1886016	.	terminator	371	411	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1886016	.	engineered_region	371	411	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1886016	.	stop_codon	404	404	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1886016	.	polyA_site	398	411	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1886016	.	stem_loop	378	397	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1886016	.	terminator	3326	3454	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1886016	.	terminator	3326	3405	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1886016	.	engineered_region	3326	3405	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1886016	.	stem_loop	3337	3380	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1886016	.	terminator	3414	3454	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1886016	.	engineered_region	3414	3454	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1886016	.	stop_codon	3447	3447	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1886016	.	polyA_site	3441	3454	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1886016	.	stem_loop	3421	3440	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1886016	.	ribosome_entry_site	628	638	.	+	0	ID=BBa_B0033;Name=BBa_B0033
BBa_K1886016	.	ribosome_entry_site	628	638	.	+	0	ID=annotation1713;Name=RBS-4\Weaker;Parent=BBa_B0033
BBa_K1886016	.	engineered_region	628	638	.	+	0	ID=annotation7028;Name=BBa_B0033;Parent=BBa_B0033
BBa_K1886016	.	ribosome_entry_site	634	637	.	+	0	ID=annotation1714;Name=RBS;Parent=BBa_B0033
BBa_K1886016	.	promoter	420	619	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_K1886016	.	start_codon	592	592	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K1886016	.	CDS	420	507	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K1886016	.	non_covalent_binding_site	508	545	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K1886016	.	engineered_region	420	619	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K1886016	.	promoter	580	585	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K1886016	.	non_covalent_binding_site	585	619	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K1886016	.	promoter	556	561	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K1886016	.	terminator	4240	4368	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1886016	.	terminator	4240	4319	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1886016	.	engineered_region	4240	4319	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1886016	.	stem_loop	4251	4294	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1886016	.	terminator	4328	4368	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1886016	.	engineered_region	4328	4368	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1886016	.	stop_codon	4361	4361	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1886016	.	polyA_site	4355	4368	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1886016	.	stem_loop	4335	4354	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1886016	.	mature_transcript_region	139	274	.	+	0	ID=BBa_K228001;Name=BBa_K228001
BBa_K1886016	.	engineered_region	3512	4231	.	+	0	ID=BBa_K895006;Name=BBa_K895006
BBa_K1886016	.	CDS	3512	4231	.	+	0	ID=annotation2194605;Name=GFP;Parent=BBa_K895006
BBa_K1886016	.	CDS	645	3317	.	+	0	ID=BBa_K228000;Name=BBa_K228000
BBa_K1886016	.	CDS	645	3317	.	+	0	ID=annotation2017714;Name=cds;Parent=BBa_K228000
BBa_K1886016	.	CDS	3463	3505	.	+	0	ID=BBa_K1321338;Name=BBa_K1321338
BBa_K1886016	.	ribosome_entry_site	3494	3505	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K1321338
BBa_K1886016	.	conserved_region	3498	3501	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1886016	.	promoter	3463	3485	.	+	0	ID=BBa_I719005;Name=BBa_I719005;Parent=BBa_K1321338
BBa_K1886016	.	promoter	1	130	.	+	0	ID=BBa_I13453;Name=BBa_I13453
>BBa_K1886016
acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacg
ctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagcaattcggagagatgccgga
gcggctgaacggaccggtctctaaaaccggagtaggggcaactctaccgggggttcaaatccccctctctccgccacta
cagatccttagcgaaagctaaggattttttttaagcttactagagccaggcatcaaataaaacgaaaggctcagtcgaa
agactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtggg
cctttctgcgtttatatactagagcaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggca
cgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccag
gctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagtcaca
caggactactagatgaccatgattaccgtgcactagaataccattaacattgctaagaacgacttctctgacatcgaac
tggctgctatcccgttcaacactctggctgaccattacggtgagcgtttagctcgcgaacagttggcccttgagcatga
gtcttacgagatgggtgaagcacgcttccgcaagatgtttgagcgtcaacttaaagctggtgaggttgcggataacgct
gccgccaagcctctcatcactaccctactccctaagatgattgcacgcatcaacgactggtttgaggaagtgaaagcta
agcgcggcaagcgcccgacagccttccagttcctgtaggaaatcaagccggaagccgtagcgtacatcaccattaagac
cactctggcttgcctaaccagtgctgacaatacaaccgttcaggctgtagcaagcgcaatcggtcgggccattgaggac
gaggctcgcttcggtcgtatccgtgaccttgaagctaagcacttcaagaaaaacgttgaggaacaactcaacaagcgcg
tagggcacgtctacaagaaagcatttatgcaagttgtcgaggctgacatgctctctaagggtctactcggtggcgaggc
gtggtcttcgtggcataaggaagactctattcatgtaggagtacgctgcatcgagatgctcattgagtcaaccggaatg
gttagcttacaccgccaaaatgctggcgtagtaggtcaagactctgagactatcgaactcgcacctgaatacgctgagg
ctatcgcaacccgtgcaggtgcgctggctggcatctctccgatgttccaaccttgcgtagttcctcctaagccgtggac
tggcattactggtggtggctattgggctaacggtcgtcgtcctctggcgctggtgcgtactcacagtaagaaagcactg
atgcgctacgaagacgtttacatgcctgaggtgtacaaagcgattaacattgcgcaaaacaccgcatggaaaatcaaca
agaaagtcctagcggtcgccaacgtaatcaccaagtggaagcattgtccggtcgaggacatccctgcgattgagcgtga
agaactcccgatgaaaccggaagacatcgacatgaatcctgaggctctcaccgcgtggaaacgtgctgccgctgctgtg
taccgcaaggacaaggctcgcaagtctcgccgtatcagccttgagttcatgcttgagcaagccaataagtttgctaacc
ataaggccatctggttcccttacaacatggactggcgcggtcgtgtttacgctgtgtcaatgttcaacccgcaaggtaa
cgatatgaccaaaggactgcttacgctggcgaaaggtaaaccaatcggtaaggaaggttactactggctgaaaatccac
ggtgcaaactgtgcgggtgtcgataaggttccgttccctgagcgcatcaagttcattgaggaaaaccacgagaacatca
tggcttgcgctaagtctccactggagaacacttggtgggctgagcaagattctccgttctgcttccttgcgttctgctt
tgagtacgctggggtacagcaccacggcctgagctataactgctcccttccgctggcgtttgacgggtcttgctctggc
atccagcacttctccgcgatgctccgagatgaggtaggtggtcgcgcggttaacttgcttcctagtgaaaccgttcagg
acatctacgggattgttgctaagaaagtcaacgagattctacaagcagacgcaatcaatgggaccgataacgaagtagt
taccgtgaccgatgagaacactggtgaaatctctgagaaagtcaagctgggcactaaggcactggctggtcaatggctg
gcttacggtgttactcgcagtgtgactaagcgttcagtcatgacgctggcttacgggtccaaagagttcggcttccgtc
aacaagtgctggaagataccattcagccagctattgattccggcaagggtctgatgttcactcagccgaatcaggctgc
tggatacatggctaagctgatttgggaatctgtgagcgtgacggtggtagctgcggttgaagcaatgaactggcttaag
tctgctgctaagctgctggctgctgaggtcaaagataagaagactggagagattcttcgcaagcgttgcgctgtgcatt
gggtaactcctgatggtttccctgtgtggcaggaatacaagaagcctattcagacgcgcttgaacctgatgttcctcgg
tcagttccgcttacagcctaccattaacaccaacaaagatagcgagattgatgcacacaaacaggagtctggtatcgct
cctaactttgtacacagccaagacggtagccaccttcgtaagactgtagtgtgggcacacgagaagtacggaatcgaat
cttttgcactgattcacgactccttcggtaccattccggctgacgctgcgaacctgttcaaagcagtgcgcgaaactat
ggttgacacatatgagtcttgtgatgtactggctgatttctacgaccagttcgctgaccagttgcacgagtctcaattg
gacaaaatgccagcacttccggctaaaggtaacttgaacctccgtgacatcttagagtcggacttcgcgttcgcataat
actagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtga
acgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtaatacgactcact
atagggagatactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattct
tgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaa
cttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtg
ttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtaca
ggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgtt
aatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataact
cacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaaga
tggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccat
tacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacag
ctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctc
agtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttc
gggtgggcctttctgcgtttata