LOCUS       BBa_K1886018            1979 bp    DNA     linear       09-Dec-2025
DEFINITION  Pompc_CI_p-lamda_sfGFP
ACCESSION   BBa_K1886018
VERSION     BBa_K1886018.1  
FEATURES             Location/Qualifiers
     misc_feature    13..30
                     /label=C1 OmpR
     misc_feature    34..51
                     /label=C2 OmpR
     misc_feature    54..71
                     /label=C3 OmpR
     promoter        75..80
                     /label=-35
     promoter        98..103
                     /label=-10
     promoter        1..108
                     /label=BBa_R0082
     misc_feature    113..116
                     /label=conserved
     RBS             109..120
                     /label=BBa_B0034
     CDS             138..845
                     /label=cI lambda
     misc_feature    846..878
                     /label=LVA
     misc_feature    885..909
                     /label=Help:Barcodes
     CDS             135..909
                     /label=BBa_C0051
     stem_loop       929..972
                     /label=stem_loop
     misc_feature    918..997
                     /label=BBa_B0010
     terminator      918..997
                     /label=BBa_B0010
     stem_loop       1013..1032
                     /label=T7 TE
     misc_feature    1039..1039
                     /label=stop
     polyA_site      1033..1046
                     /label=polya
     misc_feature    1006..1046
                     /label=BBa_B0012
     terminator      1006..1046
                     /label=BBa_B0012
     terminator      918..1046
                     /label=BBa_B0015
     promoter        1092..1097
                     /label=-10
     promoter        1069..1074
                     /label=-35
     misc_feature    1079..1095
                     /label=OR1
     misc_feature    1055..1071
                     /label=OR2
     misc_feature    1055..1103
                     /label=BBa_R0051
     promoter        1055..1103
                     /label=BBa_R0051
     misc_feature    1116..1119
                     /label=conserved
     RBS             1112..1123
                     /label=BBa_B0034
     CDS             1132..1842
                     /label=sfGFP
     CDS             1132..1842
                     /label=BBa_K1321337
     stem_loop       1862..1905
                     /label=stem_loop
     misc_feature    1851..1930
                     /label=BBa_B0010
     terminator      1851..1930
                     /label=BBa_B0010
     stem_loop       1946..1965
                     /label=T7 TE
     misc_feature    1972..1972
                     /label=stop
     polyA_site      1966..1979
                     /label=polya
     misc_feature    1939..1979
                     /label=BBa_B0012
     terminator      1939..1979
                     /label=BBa_B0012
     terminator      1851..1979
                     /label=BBa_B0015
ORIGIN
        1 tcccttgcat ttacattttg aaacatctat agcgataaat gaaacatctt aaaagtttta
       61 gtatcatatt cgtgttggat tattctgcat ttttggggag aatggactta ctagagaaag
      121 aggagaaata ctagatgagc acaaaaaaga aaccattaac acaagagcag cttgaggacg
      181 cacgtcgcct taaagcaatt tatgaaaaaa agaaaaatga acttggctta tcccaggaat
      241 ctgtcgcaga caagatgggg atggggcagt caggcgttgg tgctttattt aatggcatca
      301 atgcattaaa tgcttataac gccgcattgc ttgcaaaaat tctcaaagtt agcgttgaag
      361 aatttagccc ttcaatcgcc agagaaatct acgagatgta tgaagcggtt agtatgcagc
      421 cgtcacttag aagtgagtat gagtaccctg ttttttctca tgttcaggca gggatgttct
      481 cacctgagct tagaaccttt accaaaggtg atgcggagag atgggtaagc acaaccaaaa
      541 aagccagtga ttctgcattc tggcttgagg ttgaaggtaa ttccatgacc gcaccaacag
      601 gctccaagcc aagctttcct gacggaatgt taattctcgt tgaccctgag caggctgttg
      661 agccaggtga tttctgcata gccagacttg ggggtgatga gtttaccttc aagaaactga
      721 tcagggatag cggtcaggtg tttttacaac cactaaaccc acagtaccca atgatcccat
      781 gcaatgagag ttgttccgtt gtggggaaag ttatcgctag tcagtggcct gaagagacgt
      841 ttggcgctgc aaacgacgaa aactacgctt tagtagctta ataacgctga tagtgctagt
      901 gtagatcgct actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc
      961 ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc
     1021 ttcgggtggg cctttctgcg tttatatact agagtaacac cgtgcgtgtt gactatttta
     1081 cctctggcgg tgataatggt tgctactaga gaaagaggag aaatactaga gcgtaaaggc
     1141 gaagagctgt tcactggtgt cgtccctatt ctggtggaac tggatggtga tgtcaacggt
     1201 cataagtttt ccgtgcgtgg cgagggtgaa ggtgacgcaa ctaatggtaa actgacgctg
     1261 aagttcatct gtactactgg taaactgccg gtaccttggc cgactctggt aacgacgctg
     1321 acttatggtg ttcagtgctt tgctcgttat ccggaccata tgaagcagca tgacttcttc
     1381 aagtccgcca tgccggaagg ctatgtgcag gaacgcacga tttcctttaa ggatgacggc
     1441 acgtacaaaa cgcgtgcgga agtgaaattt gaaggcgata ccctggtaaa ccgcattgag
     1501 ctgaaaggca ttgactttaa agaagacggc aatatcctgg gccataagct ggaatacaat
     1561 tttaacagcc acaatgttta catcaccgcc gataaacaaa aaaatggcat taaagcgaat
     1621 tttaaaattc gccacaacgt ggaggatggc agcgtgcagc tggctgatca ctaccagcaa
     1681 aacactccaa tcggtgatgg tcctgttctg ctgccagaca atcactatct gagcacgcaa
     1741 agcgttctgt ctaaagatcc gaacgagaaa cgcgatcata tggttctgct ggagttcgta
     1801 accgcagcgg gcatcacgca tggtatggat gaactgtaca aatactagag ccaggcatca
     1861 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt
     1921 gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct gcgtttata
//