LOCUS       BBa_K188661             1301 bp    DNA     linear       17-May-2024
DEFINITION  ccdB
ACCESSION   BBa_K188661
VERSION     BBa_K188661.1  
FEATURES             Location/Qualifiers
     misc_feature    1..20
                     /label=Lux-box
     promoter        20..25
                     /label=-35
     misc_feature    23..40
                     /label=OR2
     promoter        42..47
                     /label=-10
     misc_feature    46..63
                     /label=OR1
     promoter        1..66
                     /label=BBa_K145150
     misc_feature    79..82
                     /label=conserved
     RBS             75..86
                     /label=BBa_B0034
     misc_feature    93..95
                     /label=start
     misc_feature    396..398
                     /label=stop
     CDS             93..395
                     /label=cds
     CDS             93..398
                     /label=BBa_K145151
     misc_feature    411..414
                     /label=conserved
     RBS             407..418
                     /label=BBa_B0034
     misc_feature    431..434
                     /label=conserved
     RBS             427..438
                     /label=BBa_B0034
     CDS             445..1119
                     /label=mrfp1
     misc_feature    1159..1164
                     /label=Stop
     misc_feature    1120..1158
                     /label=LVA
     misc_feature    445..447
                     /label=Start
     misc_feature    445..1164
                     /label=BBa_J04051
     stem_loop       1184..1227
                     /label=stem_loop
     misc_feature    1173..1252
                     /label=BBa_B0010
     terminator      1173..1252
                     /label=BBa_B0010
     stem_loop       1268..1287
                     /label=T7 TE
     misc_feature    1294..1294
                     /label=stop
     polyA_site      1288..1301
                     /label=polya
     misc_feature    1261..1301
                     /label=BBa_B0012
     terminator      1261..1301
                     /label=BBa_B0012
ORIGIN
        1 acctgtagga tcgtacaggt ttactaaaga ttcctttagt ttataattta agtgttcttt
       61 aatttctact agagaaagag gagaaatact agatgcagtt taaggtttac acctataaaa
      121 gagagagccg ttatcgtctg tttgtggatg tacagagtga tattattgac acgcccgggc
      181 gacggatggt gatccccctg gccagtgcac gtctgctgtc agataaagtc tcccgtgaac
      241 tttacccggt ggtgcatatc ggggatgaaa gctggcgcat gatgaccacc gatatggcca
      301 gtgtgccggt ctccgttatc ggggaagaag tggctgatct cagccaccgc gaaaatgaca
      361 tcaaaaacgc cattaacctg atgttctggg gaatataata ctagagaaag aggagaaata
      421 ctagagaaag aggagaaata ctagatggct tcctccgaag acgttatcaa agagttcatg
      481 cgtttcaaag ttcgtatgga aggttccgtt aacggtcacg agttcgaaat cgaaggtgaa
      541 ggtgaaggtc gtccgtacga aggtacccag accgctaaac tgaaagttac caaaggtggt
      601 ccgctgccgt tcgcttggga catcctgtcc ccgcagttcc agtacggttc caaagcttac
      661 gttaaacacc cggctgacat cccggactac ctgaaactgt ccttcccgga aggtttcaaa
      721 tgggaacgtg ttatgaactt cgaagacggt ggtgttgtta ccgttaccca ggactcctcc
      781 ctgcaagacg gtgagttcat ctacaaagtt aaactgcgtg gtaccaactt cccgtccgac
      841 ggtccggtta tgcagaaaaa aaccatgggt tgggaagctt ccaccgaacg tatgtacccg
      901 gaagacggtg ctctgaaagg tgaaatcaaa atgcgtctga aactgaaaga cggtggtcac
      961 tacgacgctg aagttaaaac cacctacatg gctaaaaaac cggttcagct gccgggtgct
     1021 tacaaaaccg acatcaaact ggacatcacc tcccacaacg aagactacac catcgttgaa
     1081 cagtacgaac gtgctgaagg tcgtcactcc accggtgcta ggcctgctgc aaacgacgaa
     1141 aactacgctt tagtagctta ataatactag agccaggcat caaataaaac gaaaggctca
     1201 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag
     1261 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat a
//