BBa_K1887001 1 BBa_K1887001 His 2016-10-09T11:00:00Z 2016-10-13T01:59:17Z We get this part by PCR using the L. lactis genomic DNA as the template. The His operon is required for the histidine biosynthesis in L. lactis,the His operon contains several genes.Here we chose one segment of the His operon for device knocked into the genome of L.lactis. In other words, the His segment is used as the flanking sequences for homologous recombination. false false _2352_ 31551 29384 9 false The devices can be inserted into the His segment by the AscI restriction enzyme site. false Yifei Lu BBa_K1887001_sequence 1 taaagtaattttcatcaattttttctaagccttcttcgcgctccttgcgcgtgacaagtaaattatttttgtcagcattatatccatcaaacttttttgcatagctaaataaggcagaatcaccttctttacgaatagcattgacaatttcttgaacacttttatttacttcttctgaaacttcggcttttcgtccttgaaatttatttgcaacttcttcaagacttccttgataatcaatttgtttgagcattttctaatctctccactatttctatgattttatcttttttgaatttgaaacttactttattaacaatcattcgggttgaaatttgactgattttttcaatcacgactaagccatttgctgacagtgtatttccagtttcgacaatatcaacaatagcatctgctaaaccaactactggaccaagctcaacagacccttctaatttgataatctcaatatcctcttgcttttgagcaaaataatttttagtgattcttggatatttagaagcaattcgcttacgcctgtgaaattttttatttaaaaaatctggataggaagcaagggcaaagatgcactgaccaacttttaaatctaataactcataataatcatcaaaatcattttcatcaagcgtatctttaccgacaaatccaatatcaacaattccatgctccaaaaaagttatgacatcattggcttttccaaaaataatttccaaatcatctggtatggttaccaccaactcacgccccatttcgcgcaccgccgtcatatcaaatcctgcctgttctagcaaaatcgtgacctgcttttgaatgcgccctttggtcattgctactctaatcatatctgtctcctatctgcccaaagggcgagcagatagcttgcttacgcaagcttctatccgcaacctgaaagctcccccttggtcgcctttgacagcttgcttgcgcaagcttctatccgcaacctgaaagctcccccttggtcgcctttgacagcttgcttgcgcaagcttctatccgcaacctgaaagctccctcttggtcgcttccagccattggtcattgctattctaatcattattcaaccctttcacaacattgtccatatgcacgcaaaagccaattgagcccgtggttttaccgaactgttttagtaacttgtcatagcgcccaccggatatagtcgcttgcgccacgccttctacgtaaacctgaaacatcagcccagtatagtagcccatcgcaggtaccatggcaaaatcatagtgaatctggcaaatcgctgtcttgtcaggtaaattcagcgcttcaagcatttcgagtgcctgagacaatttttcatcgcctgttgccaaaactaattttttcatggttggcaaatcggtcgtggtcatcaaagcaagcaacaagtcgcgtggcgcgcctatcatttcatgcttgtccacgaaagctttcatacctgagagtgattttttctcaagaagttccgaaaaaagttgcgcatctccacctgacagctcacataaacgcttataaaaagcagccgaaccaatttctacgatagtgtttttcaatgttagagcctctaagctcataaaagtcagtcctaaaatctcctgatctcctgtcacttctgacaatcctaacaattctgtcccgacctgatagctctccgtccgacgtcccttatgacgcttttctttacgaaacaccttaccaaaataactataccgggcttctgtcagctctgataaggcaaaatttcgtgccaaagggactgtaaaatcataacgaagcgcaatcgatttaccctcatgattgataaattgaaacatcttctcttgctcaaatcctgcgtcaaggccagtataaagctcgacatattcaaaattcggtggcatgacctcctgataattttgactttcaaacaagttccgcaacttctgttctatcttgcgaagtgtcgtaattcctgacaatgtcatttcacccgattcttcggggagcaaatagttcatttttttcatcagtaacttttctccttgttttttaaatattcctttaagaattgaatcactgtgttcatttctctatcagtgccaatcgtaatccgtaaccaatcagaaattcgtgcttgattccaatgtctgacaataatttttgcttcatagagtgccttgaaaagtgttgtagcatctactttgggatgatgaacgaaaacaaaatttgttttagaatcagtcacttgaaaaccaagctttatcaattcctttttgaaattctcacgagtagtaataattttactgacagacttttgaaaataagtttcgtcatttaaactcgcttcgccaattttttgcgccaagctatcaattggataagaattaaaagaatttttcatatcatacaaatgagcaattgcttcggctgagccaaaggcgactcccaaac igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z