LOCUS       BBa_K188900              879 bp    DNA     linear       18-Feb-2025
DEFINITION  1234
ACCESSION   BBa_K188900
VERSION     BBa_K188900.1  
FEATURES             Location/Qualifiers
     misc_feature    1..20
                     /label=Lux-box
     promoter        20..25
                     /label=-35
     misc_feature    23..40
                     /label=OR2
     promoter        42..47
                     /label=-10
     misc_feature    46..63
                     /label=OR1
     promoter        1..66
                     /label=BBa_K145150
     misc_feature    71..74
                     /label=conserved
     RBS             67..78
                     /label=BBa_B0034
     CDS             79..753
                     /label=mrfp1
     misc_feature    760..784
                     /label=Help:Barcodes
     CDS             79..784
                     /label=BBa_E1010
     stem_loop       792..811
                     /label=T7 TE
     misc_feature    818..818
                     /label=stop
     polyA_site      812..825
                     /label=polya
     misc_feature    785..825
                     /label=BBa_B0012
     terminator      785..825
                     /label=BBa_B0012
     stem_loop       846..868
                     /label=stem_loop
     misc_feature    834..879
                     /label=BBa_B0011
     terminator      834..879
                     /label=BBa_B0011
     terminator      785..879
                     /label=BBa_B0014
ORIGIN
        1 acctgtagga tcgtacaggt ttactaaaga ttcctttagt ttataattta agtgttcttt
       61 aatttcaaag aggagaaaat ggcttcctcc gaagacgtta tcaaagagtt catgcgtttc
      121 aaagttcgta tggaaggttc cgttaacggt cacgagttcg aaatcgaagg tgaaggtgaa
      181 ggtcgtccgt acgaaggtac ccagaccgct aaactgaaag ttaccaaagg tggtccgctg
      241 ccgttcgctt gggacatcct gtccccgcag ttccagtacg gttccaaagc ttacgttaaa
      301 cacccggctg acatcccgga ctacctgaaa ctgtccttcc cggaaggttt caaatgggaa
      361 cgtgttatga acttcgaaga cggtggtgtt gttaccgtta cccaggactc ctccctgcaa
      421 gacggtgagt tcatctacaa agttaaactg cgtggtacca acttcccgtc cgacggtccg
      481 gttatgcaga aaaaaaccat gggttgggaa gcttccaccg aacgtatgta cccggaagac
      541 ggtgctctga aaggtgaaat caaaatgcgt ctgaaactga aagacggtgg tcactacgac
      601 gctgaagtta aaaccaccta catggctaaa aaaccggttc agctgccggg tgcttacaaa
      661 accgacatca aactggacat cacctcccac aacgaagact acaccatcgt tgaacagtac
      721 gaacgtgctg aaggtcgtca ctccaccggt gcttaataac gctgatagtg ctagtgtaga
      781 tcgctcacac tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagagagaat
      841 ataaaaagcc agattattaa tccggctttt ttattattt
//