LOCUS       BBa_K1889000            1770 bp    DNA     linear       22-Dec-2024
DEFINITION  Constitutive Promoter Expressing PAL gene
ACCESSION   BBa_K1889000
VERSION     BBa_K1889000.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23100
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     CDS             62..1633
                     /label=BBa_K1129003
     stem_loop       1653..1696
                     /label=stem_loop
     misc_feature    1642..1721
                     /label=BBa_B0010
     terminator      1642..1721
                     /label=BBa_B0010
     stem_loop       1737..1756
                     /label=T7 TE
     misc_feature    1763..1763
                     /label=stop
     polyA_site      1757..1770
                     /label=polya
     misc_feature    1730..1770
                     /label=BBa_B0012
     terminator      1730..1770
                     /label=BBa_B0012
     terminator      1642..1770
                     /label=BBa_B0015
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagaaagagg agaaatacta
       61 gatgaccttc gtcatagagc tcgacatgaa cgtcacgctc gaccaacttg aggacgcggc
      121 gcgacagcgc acgcccgtgg agctgtccgc acccgtccgc tcccgcgtcc gcgcctcgcg
      181 cgacgtgttg gtgaagttcg tgcaggacga acgtgtcatc tacggggtca acaccagcat
      241 ggggggcttc gtcgaccacc tcgtcccggt gtcccaggcc cggcagctcc aggagaacct
      301 gatcaacgcg gtcgccacca acgtgggggc gtatctggac gacacgaccg cccggaccat
      361 catgctgtcc cgcatcgtgt cgctggcgcg cgggaactcc gcgatcaccc cggcgaatct
      421 ggacaagctg gtggccgtac tcaacgccgg gatcgtgccg tgcatcccgg agaagggctc
      481 tttgggcacc agcggtgacc tcggcccgct ggccgcgatc gccctggtgt gcgcggggca
      541 gtggaaggcc cgctacaacg gtcagatcat gcccgggcgg caggccctgt ccgaggccgg
      601 cgtcgagccg atggagctga gctacaagga tggcctggcc ctgatcaacg gcacgtcagg
      661 catggtcggc ctgggcacca tggtcctcca ggccgcgcgc cggctcgtgg accgctacct
      721 tcaggtgtcc gcgttgtcgg tcgagggcct ggcaggcatg acgaaaccgt tcgaccctcg
      781 cgtgcacggc gtcaagccgc accgcgggca gcgtcaggtg gcctcgcggt tgtgggaggg
      841 gcttgccgac tcgcacctgg cggtcaacga actggacacc gagcagaccc tggccggaga
      901 gatgggcacg gtcgccaagg ccggttcgct ggcgatcgag gacgcctact ccatccggtg
      961 cacgccgcag atcctcggtc ccgtggtcga tgtgctggac cggatcgggg cgacccttca
     1021 ggacgagctg aactcctcca acgacaaccc gatcgtcctg ccggaggagg cggaggtgtt
     1081 ccacaacggg cacttccacg gccagtacgt ggccatggcc atggaccacc tgaacatggc
     1141 cctggccacc gtgaccaatc tcgccaaccg gcgcgtggac cgcttcctgg acaagagcaa
     1201 cagcaacggg ctgcccgcct tcctgtgccg ggaagatccg ggactgcgcc tgggcctgat
     1261 gggcggccag ttcatgaccg cgtcgatcac cgcggagacc cgcaccctga ccattccgat
     1321 gtcggtgcag tccctcacga gtacggcgga cttccaggac atcgtgtcct tcggattcgt
     1381 cgccgcccgc cgcgcccggg aggtactcac caacgctgcc tacgtggtgg ccttcgagct
     1441 gctgtgcgcc tgccaggccg tcgacatccg cggcgcggac aaactgtcct ccttcacccg
     1501 cccgctctat gagcgcaccc gcaagatcgt gccgttcttc gaccgggacg agaccatcac
     1561 cgactacgtc gagaagctgg cggccgacct gatcgcgggc gagcccgtcg acgctgccgt
     1621 ggcggcgcac tgatactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact
     1681 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct
     1741 caccttcggg tgggcctttc tgcgtttata
//