BBa_K1889007 1 BBa_K1889007 Express amino acid deaminase under constitutive promoter 2016-10-08T11:00:00Z 2016-10-09T07:38:27Z The sequence of the gene was referenced from an article from Journal of Bacteriology, titled ???Proteus mirabilis amino acid deaminase: cloning, nucleotide sequence, and characterization of aad.??? The DNA sequence was slightly modified to allow its chemical synthesis by IDT while still retaining the original amino acid sequence. Massad, G., Zhao, H., & Mobley, H. L. (1995). Proteus mirabilis amino acid deaminase: cloning, nucleotide sequence, and characterization of aad. Journal of Bacteriology, 177(20), 5878???5883. K1889007 is a gene coding for an amino acid deaminase gene. It is derived from Proteus mirabilis. It functions to turn phenylalanine into phenylpyruvic acid by removing its amino group, coming from the enzyme family that catalyzes the breakdown of amino acids into alpha-keto acids. In our experiment, this gene is used to turn phenylalanine, a naturally occurring amino acid in E. coli, into phenylpyruvic acid. The phenylpyruvic acid then acts as an attractant to C. elegans. false false _2354_ 19079 19079 9 false ... false Lee Chi Hong component2489526 1 BBa_J23100 component2489528 1 BBa_B0034 component2489529 1 BBa_K1889006 component2489536 1 BBa_B0015 annotation2489536 1 BBa_B0015 range2489536 1 1492 1620 annotation2489526 1 BBa_J23100 range2489526 1 1 35 annotation2489529 1 BBa_K1889006 range2489529 1 62 1483 annotation2489528 1 BBa_B0034 range2489528 1 44 55 BBa_K1889006 1 BBa_K1889006 Protein Coding Sequence for Amino Acid Deaminase Gene 2016-10-08T11:00:00Z 2016-10-09T07:19:41Z . . false false _2354_ 19079 19079 9 false . false Lee Chi Hong BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1690 1 polya range1690 1 28 41 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1686 1 T7 TE range1686 1 8 27 annotation1687 1 stop range1687 1 34 34 BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916610 1 BBa_B0010 component1916612 1 BBa_B0012 annotation1916610 1 BBa_B0010 range1916610 1 1 80 annotation1916612 1 BBa_B0012 range1916612 1 89 129 BBa_J23100 1 BBa_J23100 constitutive promoter family member 2006-08-03T11:00:00Z 2015-08-31T04:08:40Z Isolated from library of promoters Released HQ 2013 Replace later false true _52_ 0 483 95 In stock true N/A true John Anderson BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_J23100_sequence 1 ttgacggctagctcagtcctaggtacagtgctagc BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K1889006_sequence 1 atgaatattagtcgtcgtaagttgttattaggggtaggggccgccggcgttctggcaggaggtgcggctttggtaccgatggtacgccgcgacggaaagtttgtagaagctaaatcacgtgcgagctttgtcgaaggtacccaaggggcgctgcctaaagaagctgacgtagtaatcatcggagctggtatccagggtattatgacggctattaatttggctgaacgcggaatgtcagttacaattcttgaaaaaggtcagattgcgggcgagcagtctggacgtgcgtacagtcagatcatttcgtaccagacaagcccagaaatcttcccgttgcatcattacggaaaaattttatggcgtggcatgaacgaaaaaattggcgctgatactagctatcgtacgcaaggacgtgtggaagctctggcagacgaaaaggctttggataaagcgcaggcgtggattaaaactgcgaaggaggcggcaggtttcgacacaccattgaatacccgtattattaaaggcgaggagctgtcgaaccgcttggtaggtgcgcagacaccctggacggtagcagcgtttgaagaagacagcggaagcgttgatccagaaactggcacaccggctttagcacgttacgccaagcaaatcggcgttaaaatctacacgaattgcgccgtgcgcgggattgaaactgccggcggcaaaatctccgacgtggtctccgagaaaggagccatcaagacgagtcaagtcgtcctggcgggtgggatctggtcgcgtctttttatgggtaacatggggattgacatcccaacgttaaatgtgtatctgtcccaacaacgtgtctcgggtgttcccggcgctccacgcgggaatgttcatcttcccaatggaatccatttccgcgagcaggctgatggcacgtatgccgtcgcgcctcgtatttttacaagttccattgtaaaggactcgttccttcttggtccgaaatttatgcatttgcttggtgggggtgagcttccccttgagttttctatcggagaggacctttttaactctttcaaaatgccaacttcgtggaatcttgatgagaaaacccccttcgagcaatttcgcgtagctaccgcgacccagaacacacagcacttagatgcggtattccaacgcatgaagaccgagttccctgtgtttgagaaaagtgaggtagttgagcgctggggtgctgttgtgtctcctaccttcgacgagttaccgatcatttcagaagttaaagaatatccaggtttggtgatcaacaccgctacagtgtggggtatgactgaagggccggcggccggcgaggttactgccgacattgtgatgggcaagaagcccgtcattgatccgacccctttttcactggatcgtttcaaaaaataa BBa_K1889007_sequence 1 ttgacggctagctcagtcctaggtacagtgctagctactagagaaagaggagaaatactagatgaatattagtcgtcgtaagttgttattaggggtaggggccgccggcgttctggcaggaggtgcggctttggtaccgatggtacgccgcgacggaaagtttgtagaagctaaatcacgtgcgagctttgtcgaaggtacccaaggggcgctgcctaaagaagctgacgtagtaatcatcggagctggtatccagggtattatgacggctattaatttggctgaacgcggaatgtcagttacaattcttgaaaaaggtcagattgcgggcgagcagtctggacgtgcgtacagtcagatcatttcgtaccagacaagcccagaaatcttcccgttgcatcattacggaaaaattttatggcgtggcatgaacgaaaaaattggcgctgatactagctatcgtacgcaaggacgtgtggaagctctggcagacgaaaaggctttggataaagcgcaggcgtggattaaaactgcgaaggaggcggcaggtttcgacacaccattgaatacccgtattattaaaggcgaggagctgtcgaaccgcttggtaggtgcgcagacaccctggacggtagcagcgtttgaagaagacagcggaagcgttgatccagaaactggcacaccggctttagcacgttacgccaagcaaatcggcgttaaaatctacacgaattgcgccgtgcgcgggattgaaactgccggcggcaaaatctccgacgtggtctccgagaaaggagccatcaagacgagtcaagtcgtcctggcgggtgggatctggtcgcgtctttttatgggtaacatggggattgacatcccaacgttaaatgtgtatctgtcccaacaacgtgtctcgggtgttcccggcgctccacgcgggaatgttcatcttcccaatggaatccatttccgcgagcaggctgatggcacgtatgccgtcgcgcctcgtatttttacaagttccattgtaaaggactcgttccttcttggtccgaaatttatgcatttgcttggtgggggtgagcttccccttgagttttctatcggagaggacctttttaactctttcaaaatgccaacttcgtggaatcttgatgagaaaacccccttcgagcaatttcgcgtagctaccgcgacccagaacacacagcacttagatgcggtattccaacgcatgaagaccgagttccctgtgtttgagaaaagtgaggtagttgagcgctggggtgctgttgtgtctcctaccttcgacgagttaccgatcatttcagaagttaaagaatatccaggtttggtgatcaacaccgctacagtgtggggtatgactgaagggccggcggccggcgaggttactgccgacattgtgatgggcaagaagcccgtcattgatccgacccctttttcactggatcgtttcaaaaaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0034_sequence 1 aaagaggagaaa BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z