LOCUS BBa_K1889021 4903 bp DNA linear 26-Nov-2024 DEFINITION Constitutive Promoter Expressing PAL, 4CL and AtCCR1 gene ACCESSION BBa_K1889021 VERSION BBa_K1889021.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23100 misc_feature 48..51 /label=conserved RBS 44..55 /label=BBa_B0034 CDS 62..1633 /label=BBa_K1129003 stem_loop 1653..1696 /label=stem_loop misc_feature 1642..1721 /label=BBa_B0010 terminator 1642..1721 /label=BBa_B0010 stem_loop 1737..1756 /label=T7 TE misc_feature 1763..1763 /label=stop polyA_site 1757..1770 /label=polya misc_feature 1730..1770 /label=BBa_B0012 terminator 1730..1770 /label=BBa_B0012 terminator 1642..1770 /label=BBa_B0015 misc_feature 1..1770 /label=BBa_K1889000 promoter 1779..1813 /label=BBa_J23100 misc_feature 1826..1829 /label=conserved RBS 1822..1833 /label=BBa_B0034 misc_feature 1840..1842 /label=Start codon misc_feature 3523..3525 /label=Stop codon CDS 1840..3522 /label=4CL CDS 1840..3525 /label=BBa_K801093 stem_loop 3545..3588 /label=stem_loop misc_feature 3534..3613 /label=BBa_B0010 terminator 3534..3613 /label=BBa_B0010 stem_loop 3629..3648 /label=T7 TE misc_feature 3655..3655 /label=stop polyA_site 3649..3662 /label=polya misc_feature 3622..3662 /label=BBa_B0012 terminator 3622..3662 /label=BBa_B0012 terminator 3534..3662 /label=BBa_B0015 misc_feature 1779..3662 /label=BBa_K1889001 promoter 3671..3705 /label=BBa_J23100 misc_feature 3718..3721 /label=conserved RBS 3714..3725 /label=BBa_B0034 CDS 3732..4766 /label=BBa_K1129005 stem_loop 4786..4829 /label=stem_loop misc_feature 4775..4854 /label=BBa_B0010 terminator 4775..4854 /label=BBa_B0010 stem_loop 4870..4889 /label=T7 TE misc_feature 4896..4896 /label=stop polyA_site 4890..4903 /label=polya misc_feature 4863..4903 /label=BBa_B0012 terminator 4863..4903 /label=BBa_B0012 terminator 4775..4903 /label=BBa_B0015 misc_feature 3671..4903 /label=BBa_K1889002 ORIGIN 1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagaaagagg agaaatacta 61 gatgaccttc gtcatagagc tcgacatgaa cgtcacgctc gaccaacttg aggacgcggc 121 gcgacagcgc acgcccgtgg agctgtccgc acccgtccgc tcccgcgtcc gcgcctcgcg 181 cgacgtgttg gtgaagttcg tgcaggacga acgtgtcatc tacggggtca acaccagcat 241 ggggggcttc gtcgaccacc tcgtcccggt gtcccaggcc cggcagctcc aggagaacct 301 gatcaacgcg gtcgccacca acgtgggggc gtatctggac gacacgaccg cccggaccat 361 catgctgtcc cgcatcgtgt cgctggcgcg cgggaactcc gcgatcaccc cggcgaatct 421 ggacaagctg gtggccgtac tcaacgccgg gatcgtgccg tgcatcccgg agaagggctc 481 tttgggcacc agcggtgacc tcggcccgct ggccgcgatc gccctggtgt gcgcggggca 541 gtggaaggcc cgctacaacg gtcagatcat gcccgggcgg caggccctgt ccgaggccgg 601 cgtcgagccg atggagctga gctacaagga tggcctggcc ctgatcaacg gcacgtcagg 661 catggtcggc ctgggcacca tggtcctcca ggccgcgcgc cggctcgtgg accgctacct 721 tcaggtgtcc gcgttgtcgg tcgagggcct ggcaggcatg acgaaaccgt tcgaccctcg 781 cgtgcacggc gtcaagccgc accgcgggca gcgtcaggtg gcctcgcggt tgtgggaggg 841 gcttgccgac tcgcacctgg cggtcaacga actggacacc gagcagaccc tggccggaga 901 gatgggcacg gtcgccaagg ccggttcgct ggcgatcgag gacgcctact ccatccggtg 961 cacgccgcag atcctcggtc ccgtggtcga tgtgctggac cggatcgggg cgacccttca 1021 ggacgagctg aactcctcca acgacaaccc gatcgtcctg ccggaggagg cggaggtgtt 1081 ccacaacggg cacttccacg gccagtacgt ggccatggcc atggaccacc tgaacatggc 1141 cctggccacc gtgaccaatc tcgccaaccg gcgcgtggac cgcttcctgg acaagagcaa 1201 cagcaacggg ctgcccgcct tcctgtgccg ggaagatccg ggactgcgcc tgggcctgat 1261 gggcggccag ttcatgaccg cgtcgatcac cgcggagacc cgcaccctga ccattccgat 1321 gtcggtgcag tccctcacga gtacggcgga cttccaggac atcgtgtcct tcggattcgt 1381 cgccgcccgc cgcgcccggg aggtactcac caacgctgcc tacgtggtgg ccttcgagct 1441 gctgtgcgcc tgccaggccg tcgacatccg cggcgcggac aaactgtcct ccttcacccg 1501 cccgctctat gagcgcaccc gcaagatcgt gccgttcttc gaccgggacg agaccatcac 1561 cgactacgtc gagaagctgg cggccgacct gatcgcgggc gagcccgtcg acgctgccgt 1621 ggcggcgcac tgatactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact 1681 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct 1741 caccttcggg tgggcctttc tgcgtttata tactagagtt gacggctagc tcagtcctag 1801 gtacagtgct agctactaga gaaagaggag aaatactaga tggcgccaca agaacaagca 1861 gtttctcagg tgatggagaa acagagcaac aacaacaaca gtgacgtcat tttccgatca 1921 aagttaccgg atatttacat cccgaaccac ctatctctcc acgactacat cttccaaaac 1981 atctccgagt tcgccactaa gccttgccta atcaacggac caaccggcca cgtgtacact 2041 tactccgacg tccacgtcat ctcccgccaa atcgccgcca attttcacaa actcggcgtt 2101 aaccaaaacg acgtcgtcat gctcctcctc ccaaactgtc ccgagttcgt cctctctttc 2161 ctcgccgcct ccttccgcgg cgcaaccgcc accgccgcaa accctttctt cactccggcg 2221 gagatagcta aacaagccaa agcctccaac accaaactca taatcaccga agctcgttac 2281 gtcgacaaaa tcaaaccact tcaaaacgac gacggagtag tcatcgtctg catcgacgac 2341 aacgaatccg tgccaatccc tgaaggctgc ctccgcttca ccgagttgac tcagtcgaca 2401 accgaggcat cagaagtcat cgactcggtg gagatttcac cggacgacgt ggtggcacta 2461 ccttactcct ctggcacgac gggattacca aaaggagtga tgctgactca caagggtcta 2521 gtcacgagcg ttgctcagca agtcgacggc gagaacccga atctttattt ccacagcgat 2581 gacgtcatac tctgtgtttt gcccatgttt catatctacg ctttgaactc gatcatgttg 2641 tgtggtctta gagttggtgc ggcgattctg ataatgccga agtttgagat caatctgcta 2701 ttggagctga tccagaggtg taaagtgacg gtggctccga tggttccgcc gattgtgttg 2761 gccattgcga agtcttcgga gacggagaag tatgatttga gctcgataag agtggtgaaa 2821 tctggtgctg ctcctcttgg taaagaactt gaagatgccg ttaatgccaa gtttcctaat 2881 gccaaactcg gtcagggata cggaatgacg gaagcaggtc cagtgctagc aatgtcgtta 2941 ggttttgcaa aggaaccttt tccggttaag tcaggagctt gtggtactgt tgtaagaaat 3001 gctgagatga aaatagttga tccagacacc ggagattctc tttcgaggaa tcaacccggt 3061 gagatttgta ttcgtggtca ccagatcatg aaaggttacc tcaacaatcc ggcagctaca 3121 gcagagacca ttgataaaga cggttggctt catactggag atattggatt gatcgatgac 3181 gatgacgagc ttttcatcgt tgatcgattg aaagaactta tcaagtataa aggttttcag 3241 gtagctccgg ctgagctaga ggctttgctc atcggtcatc ctgacattac tgatgttgct 3301 gttgtcgcaa tgaaagaaga agcagctggt gaagttcctg ttgcatttgt ggtgaaatcg 3361 aaggattcgg agttatcaga agatgatgtg aagcaattcg tgtcgaaaca ggttgtgttt 3421 tacaagagaa tcaacaaagt gttcttcact gaatccattc ctaaagctcc atcagggaag 3481 atattgagga aagatctgag ggcaaaacta gcaaatggat tgtaatacta gagccaggca 3541 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc 3601 ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta 3661 tatactagag ttgacggcta gctcagtcct aggtacagtg ctagctacta gagaaagagg 3721 agaaatacta gatgccagtc gacgtagcct caccggccgg aaaaaccgtc tgcgtcaccg 3781 gagctggtgg atacatcgct tcttggattg ttaagatact tctcgagaga ggttacacag 3841 tcaaaggaac cgtacggaat ccagatgatc cgaagaacac acatttgaga gaactagaag 3901 gaggaaagga gagactgatt ctgtgcaaag cagatcttca ggactacgag gctcttaagg 3961 cggcgattga tggttgcgac ggcgtctttc acacggcttc tcctgtcacc gacgatccgg 4021 aacaaatggt ggagccggcc gtgaatggag ccaagtttgt aattaatgct gcggctgagg 4081 ccaaggtcaa gcgcgtggtc atcacctcct ccattggtgc cgtctacatg gacccgaacc 4141 gtgaccctga ggctgtcgtt gacgaaagtt gttggagtga tcttgacttc tgcaaaaaca 4201 ccaagaattg gtattgttac ggcaagatgg tggcggaaca agcggcgtgg gagacagcaa 4261 aggagaaagg tgttgacttg gtggtgttga atccggtgct ggttcttgga ccgccgttac 4321 agccgacgat caacgccagt ctttaccacg tcctcaaata tctaaccggc tcggctaaga 4381 cttatgctaa tttgactcaa gcttatgtgg atgttcgcga tgtcgcgctg gctcatgttc 4441 tggtctatga ggcaccctcg gcctccggac gttatctcct agccgagagt gctcgccacc 4501 gcggggaagt tgttgagatt ctggctaagc tattcccgga gtatcctctt ccgaccaagt 4561 gcaaggacga gaagaaccct agagccaagc catacaaatt cactaaccag aagattaagg 4621 acttaggctt agagttcact tccaccaagc aaagcctcta cgacacagtc aagagcttac 4681 aagagaaagg ccatcttgct cctcctcctc ctcctccttc agcatcgcaa gaatccgtgg 4741 aaaatggcat taagatcggg tcttgatact agagccaggc atcaaataaa acgaaaggct 4801 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag 4861 agtcacactg gctcaccttc gggtgggcct ttctgcgttt ata //