LOCUS       BBa_K188903             1946 bp    DNA     linear       22-Nov-2024
DEFINITION  test
ACCESSION   BBa_K188903
VERSION     BBa_K188903.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     misc_feature    81..82
                     /label=prefix
     misc_feature    254..254
                     /label=T
     misc_feature    572..572
                     /label=A
     CDS             81..830
                     /label=luxR
     misc_feature    81..836
                     /label=BBa_C0062
     misc_feature    837..861
                     /label=Help:Barcodes
     CDS             81..836
                     /label=BBa_C0062
     stem_loop       881..924
                     /label=stem_loop
     misc_feature    870..949
                     /label=BBa_B0010
     terminator      870..949
                     /label=BBa_B0010
     stem_loop       965..984
                     /label=T7 TE
     misc_feature    991..991
                     /label=stop
     polyA_site      985..998
                     /label=polya
     misc_feature    958..998
                     /label=BBa_B0012
     terminator      958..998
                     /label=BBa_B0012
     terminator      870..998
                     /label=BBa_B0015
     misc_feature    1007..1026
                     /label=LuxR/HSL
     promoter        1026..1031
                     /label=-35
     promoter        1048..1053
                     /label=-10
     misc_feature    1059..1059
                     /label=start
     misc_feature    1007..1061
                     /label=BBa_R0062
     promoter        1007..1061
                     /label=BBa_R0062
     misc_feature    1..1061
                     /label=BBa_F2620
     RBS             1062..1074
                     /label=RBS-3\Weak
     RBS             1068..1071
                     /label=RBS
     misc_feature    1062..1074
                     /label=BBa_B0032
     RBS             1062..1074
                     /label=BBa_B0032
     misc_feature    1075..1077
                     /label=start
     misc_feature    1828..1830
                     /label=stop
     misc_feature    1831..1833
                     /label=stop
     misc_feature    1791..1827
                     /label=LVA tag
     CDS             1078..1790
                     /label=cds
     misc_feature    1075..1833
                     /label=BBa_K145015
     terminator      1834..1946
                     /label=BBa_J61048
     misc_feature    1062..1946
                     /label=BBa_K188902
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgaaaaaca taaatgccga cgacacatac agaataatta
      121 ataaaattaa agcttgtaga agcaataatg atattaatca atgcttatct gatatgacta
      181 aaatggtaca ttgtgaatat tatttactcg cgatcattta tcctcattct atggttaaat
      241 ctgatatttc aatcctagat aattacccta aaaaatggag gcaatattat gatgacgcta
      301 atttaataaa atatgatcct atagtagatt attctaactc caatcattca ccaattaatt
      361 ggaatatatt tgaaaacaat gctgtaaata aaaaatctcc aaatgtaatt aaagaagcga
      421 aaacatcagg tcttatcact gggtttagtt tccctattca tacggctaac aatggcttcg
      481 gaatgcttag ttttgcacat tcagaaaaag acaactatat agatagttta tttttacatg
      541 cgtgtatgaa cataccatta attgttcctt ctctagttga taattatcga aaaataaata
      601 tagcaaataa taaatcaaac aacgatttaa ccaaaagaga aaaagaatgt ttagcgtggg
      661 catgcgaagg aaaaagctct tgggatattt caaaaatatt aggttgcagt gagcgtactg
      721 tcactttcca tttaaccaat gcgcaaatga aactcaatac aacaaaccgc tgccaaagta
      781 tttctaaagc aattttaaca ggagcaattg attgcccata ctttaaaaat taataacact
      841 gatagtgcta gtgtagatca ctactagagc caggcatcaa ataaaacgaa aggctcagtc
      901 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca
      961 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagacct gtaggatcgt
     1021 acaggtttac gcaagaaaat ggtttgttat agtcgaataa atcacacagg aaagatgcgt
     1081 aaaggagaag aacttttcac tggagttgtc ccaattcttg ttgaattaga tggtgatgtt
     1141 aatgggcaca aattttctgt cagtggagag ggtgaaggtg atgcaacata cggaaaactt
     1201 acccttaaat ttatttgcac tactggaaaa ctacctgttc catggccaac acttgtcact
     1261 actttcggtt atggtgttca atgctttgcg agatacccag atcatatgaa acagcatgac
     1321 tttttcaaga gtgccatgcc cgaaggttat gtacaggaaa gaactatatt tttcaaagat
     1381 gacgggaact acaagacacg tgctgaagtc aagtttgaag gtgataccct tgttaataga
     1441 atcgagttaa aaggtattga ttttaaagaa gatggaaaca ttcttggaca caaattggaa
     1501 tacaactata actcacacaa tgtatacatc atggcagaca aacaaaagaa tggaatcaaa
     1561 gttaacttca aaattagaca caacattgaa gatggaagcg ttcaactagc agaccattat
     1621 caacaaaata ctccaattgg cgatggccct gtccttttac cagacaacca ttacctgtcc
     1681 acacaatctg ccctttcgaa agatcccaac gaaaagagag accacatggt ccttcttgag
     1741 tttgtaacag ctgctgggat tacacatggc atggatgaac tatacaaaag gcctgctgca
     1801 aacgacgaaa actacgcttt agtagcttaa taaccggctt atcggtcagt ttcacctgat
     1861 ttacgtaaaa acccgcttcg gcgggttttt gcttttggag gggcagaaag atgaatgact
     1921 gtccacgacg ctatacccaa aagaaa
//