BBa_K1890001 1 BBa_K1890001 Silicatein gene, fused to transmembrane domain of INP, with strong RBS 2016-09-06T11:00:00Z 2016-10-13T09:40:18Z Suberites domuncula Silicatein, originating from the demosponge Suberites domuncula, catalyzes the formation of polysilicate. The gene is fused to the transmembrane domain of outer membrane protein A (OmpA), a native protein from E. coli, together with Lpp signaling peptide. false false _2355_ 29445 29445 9 false In-frame fusion of the two domains, both codon optimized for E. coli. false Lycka Kamoen, Maria Vazquez component2498746 1 BBa_G0002 component2498748 1 BBa_B0034 component2498751 1 BBa_K1890058 component2498752 1 BBa_K1890050 component2498750 1 BBa_K1890053 component2498749 1 BBa_G0000 annotation2498746 1 BBa_G0002 range2498746 1 1 8 annotation2498750 1 BBa_K1890053 range2498750 1 27 758 annotation2498748 1 BBa_B0034 range2498748 1 9 20 annotation2498751 1 BBa_K1890058 range2498751 1 759 782 annotation2498749 1 BBa_G0000 range2498749 1 21 26 annotation2498752 1 BBa_K1890050 range2498752 1 783 1442 BBa_G0002 1 SX scar SpeI/XbaI mixed site 2007-02-26T12:00:00Z 2015-08-31T04:07:27Z XbaI and SpeI sites XbaI/SpeI mixed site. Simply used to aid in entry of parts into the registry. false true _41_ 0 126 70 Not in stock false None. false Reshma Shetty BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K1890050 1 BBa_K1890050 Silicatein from Suberites domuncula 2016-10-11T11:00:00Z 2016-10-18T01:22:19Z Suberites domuncula Silicatein, originating from the demosponge <i>Suberites domuncula</i>, catalyzes the formation of polysilicate. This biobrick contains the short version of the silicatein gene, according to M??ller <i>et al</i> [1][2]. false false _2355_ 29445 29445 9 false false Lycka Kamoen, Maria Vazquez BBa_K1890058 1 BBa_K1890058 Linker 2016-10-11T11:00:00Z 2016-10-18T01:24:12Z Linker to fuse sequences in frame false false _2355_ 29445 29445 9 false false Lycka Kamoen, Maria Vazquez BBa_K1890053 1 BBa_K1890053 INP transmembrane domain 2016-10-11T11:00:00Z 2016-10-18T01:23:09Z Pseudomonas syringae The transmembrane domain of ice nucleation protein (INP) from Pseudomonas syringae. false false _2355_ 29445 29445 9 false false Lycka Kamoen, Maria Vazquez BBa_G0000 1 scar SpeI/XbaI scar for RBS-CDS junctions 2007-07-22T11:00:00Z 2015-08-31T04:07:27Z SpeI/XbaI scar This is the sequence of the SpeI/XbaI scar for RBS-CDS junctions in BioBricks standard assembly. false true _41_ 0 126 162 Not in stock false This is a shorter scar to ensure proper spacing between the RBS and CDS. false Reshma Shetty BBa_B0034_sequence 1 aaagaggagaaa BBa_G0000_sequence 1 tactag BBa_K1890050_sequence 1 atggattaccccgaggcagtggattggcgcactaagggggcagttactgcggtgaaggatcagggagactgtggtgcatcatacgcatttagcgccatgggtgccttggaaggcgcgaacgccttagcgaaaggcaacgccgtatccctgtcggagcaaaacatcattgattgttcaattccttatggtaaccatggttgtcatggtggaaacatgtatgatgctttcttatacgtcatcgcgaatgagggagtggaccaagattcagcgtatcctttcgtcgggaaacagtcctcttgtaactacaactcgaagtataagggcacgtcgatgagtggtatggtgtctattaagtcgggatcagagtccgatttgcaagcagccgtctctaatgtcggcccagtttcggtggcaattgacggcgcaaactcggccttccgcttttattattctggcgtctatgattccagccgttgttctagttcgtctttgaatcatgcaatggtggtgacggggtacggcagttacaatggaaaaaaatactggcttgccaagaactcctggggcactaactgggggaactcagggtacgttatgatggctcgtaataaatacaatcagtgcggtattgcgacggacgcatcctatcctacgttgtaa BBa_K1890058_sequence 1 ggaggaggcagtggcggtggaagt BBa_K1890053_sequence 1 atgaccttagataaagccttggtattacgcacgtgtgctaacaatatggcagatcactgcgggttaatttggccggccagcggtactgtggagagccgctattggcagagtacccgccgccacgaaaacgggcttgttggccttctgtggggagccggtacgtctgcctttctgtccgtccatgccgatgcgcgttggatcgtctgcgaagtcgccgtagcggacatcatttcgttggaggaaccgggcatggtcaaattcccccgcgctgaagttgttcacgtgggtgatcgcatttcagcttcacacttcatctcggcacgccaagccgatcccgcaagcacgagcaccagcacttcaacttccaccttaacgcctatgccgaccgcgattccgacaccgatgccggccgttgcatcggttactctgccggtcgccgagcaggcccgccatgaagttttcgacgtagcgagtgtttcggccgccgctgcccccgtgaatactctgcctgtcactacgcctcagaaccttcagactgcgacttacgggagcacattatctggggataaccactctcgcttaatcttccgtttgtgggacggtaaacgttatcgccaacttgtggctcgtaccggcgagaacggggttgaggctgacatcccgtactatgtgaacgaggacgacgatattgttgataagccggacgaggacgacgactggatcgaggtgaag BBa_K1890001_sequence 1 tactagagaaagaggagaaatactagatgaccttagataaagccttggtattacgcacgtgtgctaacaatatggcagatcactgcgggttaatttggccggccagcggtactgtggagagccgctattggcagagtacccgccgccacgaaaacgggcttgttggccttctgtggggagccggtacgtctgcctttctgtccgtccatgccgatgcgcgttggatcgtctgcgaagtcgccgtagcggacatcatttcgttggaggaaccgggcatggtcaaattcccccgcgctgaagttgttcacgtgggtgatcgcatttcagcttcacacttcatctcggcacgccaagccgatcccgcaagcacgagcaccagcacttcaacttccaccttaacgcctatgccgaccgcgattccgacaccgatgccggccgttgcatcggttactctgccggtcgccgagcaggcccgccatgaagttttcgacgtagcgagtgtttcggccgccgctgcccccgtgaatactctgcctgtcactacgcctcagaaccttcagactgcgacttacgggagcacattatctggggataaccactctcgcttaatcttccgtttgtgggacggtaaacgttatcgccaacttgtggctcgtaccggcgagaacggggttgaggctgacatcccgtactatgtgaacgaggacgacgatattgttgataagccggacgaggacgacgactggatcgaggtgaagggaggaggcagtggcggtggaagtatggattaccccgaggcagtggattggcgcactaagggggcagttactgcggtgaaggatcagggagactgtggtgcatcatacgcatttagcgccatgggtgccttggaaggcgcgaacgccttagcgaaaggcaacgccgtatccctgtcggagcaaaacatcattgattgttcaattccttatggtaaccatggttgtcatggtggaaacatgtatgatgctttcttatacgtcatcgcgaatgagggagtggaccaagattcagcgtatcctttcgtcgggaaacagtcctcttgtaactacaactcgaagtataagggcacgtcgatgagtggtatggtgtctattaagtcgggatcagagtccgatttgcaagcagccgtctctaatgtcggcccagtttcggtggcaattgacggcgcaaactcggccttccgcttttattattctggcgtctatgattccagccgttgttctagttcgtctttgaatcatgcaatggtggtgacggggtacggcagttacaatggaaaaaaatactggcttgccaagaactcctggggcactaactgggggaactcagggtacgttatgatggctcgtaataaatacaatcagtgcggtattgcgacggacgcatcctatcctacgttgtaa BBa_G0002_sequence 1 tactagag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z