BBa_K1890001
1
BBa_K1890001
Silicatein gene, fused to transmembrane domain of INP, with strong RBS
2016-09-06T11:00:00Z
2016-10-13T09:40:18Z
Suberites domuncula
Silicatein, originating from the demosponge Suberites domuncula, catalyzes the formation of polysilicate. The gene is fused to the transmembrane domain of outer membrane protein A (OmpA), a native protein from E. coli, together with Lpp signaling peptide.
false
false
_2355_
29445
29445
9
false
In-frame fusion of the two domains, both codon optimized for E. coli.
false
Lycka Kamoen, Maria Vazquez
component2498746
1
BBa_G0002
component2498748
1
BBa_B0034
component2498751
1
BBa_K1890058
component2498752
1
BBa_K1890050
component2498750
1
BBa_K1890053
component2498749
1
BBa_G0000
annotation2498746
1
BBa_G0002
range2498746
1
1
8
annotation2498750
1
BBa_K1890053
range2498750
1
27
758
annotation2498748
1
BBa_B0034
range2498748
1
9
20
annotation2498751
1
BBa_K1890058
range2498751
1
759
782
annotation2498749
1
BBa_G0000
range2498749
1
21
26
annotation2498752
1
BBa_K1890050
range2498752
1
783
1442
BBa_G0002
1
SX scar
SpeI/XbaI mixed site
2007-02-26T12:00:00Z
2015-08-31T04:07:27Z
XbaI and SpeI sites
XbaI/SpeI mixed site. Simply used to aid in entry of parts into the registry.
false
true
_41_
0
126
70
Not in stock
false
None.
false
Reshma Shetty
BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_K1890050
1
BBa_K1890050
Silicatein from Suberites domuncula
2016-10-11T11:00:00Z
2016-10-18T01:22:19Z
Suberites domuncula
Silicatein, originating from the demosponge <i>Suberites domuncula</i>, catalyzes the formation of polysilicate. This biobrick contains the short version of the silicatein gene, according to M??ller <i>et al</i> [1][2].
false
false
_2355_
29445
29445
9
false
false
Lycka Kamoen, Maria Vazquez
BBa_K1890058
1
BBa_K1890058
Linker
2016-10-11T11:00:00Z
2016-10-18T01:24:12Z
Linker to fuse sequences in frame
false
false
_2355_
29445
29445
9
false
false
Lycka Kamoen, Maria Vazquez
BBa_K1890053
1
BBa_K1890053
INP transmembrane domain
2016-10-11T11:00:00Z
2016-10-18T01:23:09Z
Pseudomonas syringae
The transmembrane domain of ice nucleation protein (INP) from Pseudomonas syringae.
false
false
_2355_
29445
29445
9
false
false
Lycka Kamoen, Maria Vazquez
BBa_G0000
1
scar
SpeI/XbaI scar for RBS-CDS junctions
2007-07-22T11:00:00Z
2015-08-31T04:07:27Z
SpeI/XbaI scar
This is the sequence of the SpeI/XbaI scar for RBS-CDS junctions in BioBricks standard assembly.
false
true
_41_
0
126
162
Not in stock
false
This is a shorter scar to ensure proper spacing between the RBS and CDS.
false
Reshma Shetty
BBa_B0034_sequence
1
aaagaggagaaa
BBa_G0000_sequence
1
tactag
BBa_K1890050_sequence
1
atggattaccccgaggcagtggattggcgcactaagggggcagttactgcggtgaaggatcagggagactgtggtgcatcatacgcatttagcgccatgggtgccttggaaggcgcgaacgccttagcgaaaggcaacgccgtatccctgtcggagcaaaacatcattgattgttcaattccttatggtaaccatggttgtcatggtggaaacatgtatgatgctttcttatacgtcatcgcgaatgagggagtggaccaagattcagcgtatcctttcgtcgggaaacagtcctcttgtaactacaactcgaagtataagggcacgtcgatgagtggtatggtgtctattaagtcgggatcagagtccgatttgcaagcagccgtctctaatgtcggcccagtttcggtggcaattgacggcgcaaactcggccttccgcttttattattctggcgtctatgattccagccgttgttctagttcgtctttgaatcatgcaatggtggtgacggggtacggcagttacaatggaaaaaaatactggcttgccaagaactcctggggcactaactgggggaactcagggtacgttatgatggctcgtaataaatacaatcagtgcggtattgcgacggacgcatcctatcctacgttgtaa
BBa_K1890058_sequence
1
ggaggaggcagtggcggtggaagt
BBa_K1890053_sequence
1
atgaccttagataaagccttggtattacgcacgtgtgctaacaatatggcagatcactgcgggttaatttggccggccagcggtactgtggagagccgctattggcagagtacccgccgccacgaaaacgggcttgttggccttctgtggggagccggtacgtctgcctttctgtccgtccatgccgatgcgcgttggatcgtctgcgaagtcgccgtagcggacatcatttcgttggaggaaccgggcatggtcaaattcccccgcgctgaagttgttcacgtgggtgatcgcatttcagcttcacacttcatctcggcacgccaagccgatcccgcaagcacgagcaccagcacttcaacttccaccttaacgcctatgccgaccgcgattccgacaccgatgccggccgttgcatcggttactctgccggtcgccgagcaggcccgccatgaagttttcgacgtagcgagtgtttcggccgccgctgcccccgtgaatactctgcctgtcactacgcctcagaaccttcagactgcgacttacgggagcacattatctggggataaccactctcgcttaatcttccgtttgtgggacggtaaacgttatcgccaacttgtggctcgtaccggcgagaacggggttgaggctgacatcccgtactatgtgaacgaggacgacgatattgttgataagccggacgaggacgacgactggatcgaggtgaag
BBa_K1890001_sequence
1
tactagagaaagaggagaaatactagatgaccttagataaagccttggtattacgcacgtgtgctaacaatatggcagatcactgcgggttaatttggccggccagcggtactgtggagagccgctattggcagagtacccgccgccacgaaaacgggcttgttggccttctgtggggagccggtacgtctgcctttctgtccgtccatgccgatgcgcgttggatcgtctgcgaagtcgccgtagcggacatcatttcgttggaggaaccgggcatggtcaaattcccccgcgctgaagttgttcacgtgggtgatcgcatttcagcttcacacttcatctcggcacgccaagccgatcccgcaagcacgagcaccagcacttcaacttccaccttaacgcctatgccgaccgcgattccgacaccgatgccggccgttgcatcggttactctgccggtcgccgagcaggcccgccatgaagttttcgacgtagcgagtgtttcggccgccgctgcccccgtgaatactctgcctgtcactacgcctcagaaccttcagactgcgacttacgggagcacattatctggggataaccactctcgcttaatcttccgtttgtgggacggtaaacgttatcgccaacttgtggctcgtaccggcgagaacggggttgaggctgacatcccgtactatgtgaacgaggacgacgatattgttgataagccggacgaggacgacgactggatcgaggtgaagggaggaggcagtggcggtggaagtatggattaccccgaggcagtggattggcgcactaagggggcagttactgcggtgaaggatcagggagactgtggtgcatcatacgcatttagcgccatgggtgccttggaaggcgcgaacgccttagcgaaaggcaacgccgtatccctgtcggagcaaaacatcattgattgttcaattccttatggtaaccatggttgtcatggtggaaacatgtatgatgctttcttatacgtcatcgcgaatgagggagtggaccaagattcagcgtatcctttcgtcgggaaacagtcctcttgtaactacaactcgaagtataagggcacgtcgatgagtggtatggtgtctattaagtcgggatcagagtccgatttgcaagcagccgtctctaatgtcggcccagtttcggtggcaattgacggcgcaaactcggccttccgcttttattattctggcgtctatgattccagccgttgttctagttcgtctttgaatcatgcaatggtggtgacggggtacggcagttacaatggaaaaaaatactggcttgccaagaactcctggggcactaactgggggaactcagggtacgttatgatggctcgtaataaatacaatcagtgcggtattgcgacggacgcatcctatcctacgttgtaa
BBa_G0002_sequence
1
tactagag
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z