BBa_G0002 1 SX scar SpeI/XbaI mixed site 2007-02-26T12:00:00Z 2015-08-31T04:07:27Z XbaI and SpeI sites XbaI/SpeI mixed site. Simply used to aid in entry of parts into the registry. false true _41_ 0 126 70 Not in stock false None. false Reshma Shetty BBa_K1890002 1 BBa_K1890002 Silicatein gene, fused to transmembrane domain of OmpA, with strong RBS 2016-09-07T11:00:00Z 2016-10-16T01:23:05Z Tethya aurantia Silicatein, originating from the demosponge Tethya aurantia, catalyzes the formation of polysilicate. As described by Curnow et al, the silicatein gene was fused to the transmembrane domain of outer membrane protein A (OmpA), in order to display it at the surface of the cell. The fusion of silicatein and OmpA is constructed according to Francisco et al, consisting of the transmembrane domain of OmpA together with the signaling peptide and the first nine N-terminal amino acids of lipoprotein (Lpp). The coding sequence in this BioBrick is combined with the strong RBS. false false _2355_ 29445 29445 9 true In frame fusion, codon optimized for E. coli. false Lycka Kamoen, Maria Vazquez component2498814 1 BBa_B0034 component2498815 1 BBa_G0000 component2498816 1 BBa_K1890052 component2498817 1 BBa_K1890051 component2498812 1 BBa_G0002 annotation2498814 1 BBa_B0034 range2498814 1 9 20 annotation2498817 1 BBa_K1890051 range2498817 1 489 1481 annotation2498815 1 BBa_G0000 range2498815 1 21 26 annotation2498812 1 BBa_G0002 range2498812 1 1 8 annotation2498816 1 BBa_K1890052 range2498816 1 27 488 BBa_K1890051 1 BBa_K1890051 Silicatein from Tethya aurantia 2016-10-11T11:00:00Z 2016-10-19T03:06:17Z Tethya aurantium Silicatein, originating from the demosponge Tethya aurantium, catalyzes the formation of polysilicate. false false _2355_ 29445 29445 9 false false Lycka Kamoen, Maria Vazquez BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K1890052 1 BBa_K1890052 OmpA transmembrane domain 2016-10-11T11:00:00Z 2016-10-18T01:22:54Z E. coli The transmembrane domain of OmpA together with the signaling peptide and the first nine N-terminal amino acids of lipoprotein (Lpp), both of which are native proteins from Escherichia coli. false false _2355_ 29445 29445 9 false false Lycka Kamoen, Maria Vazquez BBa_G0000 1 scar SpeI/XbaI scar for RBS-CDS junctions 2007-07-22T11:00:00Z 2015-08-31T04:07:27Z SpeI/XbaI scar This is the sequence of the SpeI/XbaI scar for RBS-CDS junctions in BioBricks standard assembly. false true _41_ 0 126 162 Not in stock false This is a shorter scar to ensure proper spacing between the RBS and CDS. false Reshma Shetty BBa_B0034_sequence 1 aaagaggagaaa BBa_G0000_sequence 1 tactag BBa_K1890051_sequence 1 atgtatctcggcacgttggttgttttgtgtgttttgggggctgctattggagagccaatgcctcagtatgagttcaaggaggaatggcagctgtggaagaaacaacatgacaagtcttacagcaccaacttggaggaactggagaaacatcttgtctggctctccaacaagaagtacattgaactgcacaatgccaatgcagacacctttggattcactctagctatgaaccatctaggagatatgactgaccatgaatacaaggagagatacctcacatacactaacagcaaatctggtaactacaccaaggtgttcaaacgtgagccatggatggcctacccggagactgtagattggagaacaaagggcgctgtgactggtatcaagagccagggagattgtggtgccagctatgcattcagtgccatgggtgcacttgaaggaatcaatgcacttgctactggaaagctgacctatctcagtgaacagaacatcattgattgctctgtaccttatggtaaccatggttgcaagggtggaaacatgtatgtggctttcctctatgttgttgctaacgaaggagttgatgatgggggttcctatccatttagaggaaagcaatccagttgtacgtatcaagagcagtaccgtggtgcaagtatgtctggctcagttcaaatcaacagtggtagtgaatctgatctggaagcagctgtagccaatgttggtccagttgcagtagctattgatggagagtcaaatgctttcagattctattacagtggagtgtacgactcctccagatgttctagtagcagtctcaaccacgccatggtgatcactggctatggaatttcaaataaccaggaatactggcttgcaaagaacagctggggtgagaactggggagaactgggctatgtgaagatggccaggaacaagtacaatcaatgtgggattgctagtgatgcctcctaccccactctctag BBa_K1890002_sequence 1 tactagagaaagaggagaaatactagatgaaggctacaaaattggtgttgggagcggtaatcctgggttcgactctgttggcaggctgttcttccaatgcgaagatcgaccagttatccagtgatgtggggatcaacccatacgtcggtttcgagatgggttatgactggctgggccgcatgccatataagggatccgtggaaaacggcgcatataaggcacaaggagtccaattgaccgcaaagttgggttaccccattactgatgatttggacatctacacgcgtctgggtgggatggtgtggcgcgcagacacaaaaagtaatgtttatggtaagaatcacgatacaggagtttctcccgtttttgctggaggagtggagtatgcgatcacgccggaaatcgcgacgcgcttggagtaccaatggactaacaacattggcgacgcgcacacgattggcactcgtcctgacaacggcatcccaggtatgtatctcggcacgttggttgttttgtgtgttttgggggctgctattggagagccaatgcctcagtatgagttcaaggaggaatggcagctgtggaagaaacaacatgacaagtcttacagcaccaacttggaggaactggagaaacatcttgtctggctctccaacaagaagtacattgaactgcacaatgccaatgcagacacctttggattcactctagctatgaaccatctaggagatatgactgaccatgaatacaaggagagatacctcacatacactaacagcaaatctggtaactacaccaaggtgttcaaacgtgagccatggatggcctacccggagactgtagattggagaacaaagggcgctgtgactggtatcaagagccagggagattgtggtgccagctatgcattcagtgccatgggtgcacttgaaggaatcaatgcacttgctactggaaagctgacctatctcagtgaacagaacatcattgattgctctgtaccttatggtaaccatggttgcaagggtggaaacatgtatgtggctttcctctatgttgttgctaacgaaggagttgatgatgggggttcctatccatttagaggaaagcaatccagttgtacgtatcaagagcagtaccgtggtgcaagtatgtctggctcagttcaaatcaacagtggtagtgaatctgatctggaagcagctgtagccaatgttggtccagttgcagtagctattgatggagagtcaaatgctttcagattctattacagtggagtgtacgactcctccagatgttctagtagcagtctcaaccacgccatggtgatcactggctatggaatttcaaataaccaggaatactggcttgcaaagaacagctggggtgagaactggggagaactgggctatgtgaagatggccaggaacaagtacaatcaatgtgggattgctagtgatgcctcctaccccactctctag BBa_K1890052_sequence 1 atgaaggctacaaaattggtgttgggagcggtaatcctgggttcgactctgttggcaggctgttcttccaatgcgaagatcgaccagttatccagtgatgtggggatcaacccatacgtcggtttcgagatgggttatgactggctgggccgcatgccatataagggatccgtggaaaacggcgcatataaggcacaaggagtccaattgaccgcaaagttgggttaccccattactgatgatttggacatctacacgcgtctgggtgggatggtgtggcgcgcagacacaaaaagtaatgtttatggtaagaatcacgatacaggagtttctcccgtttttgctggaggagtggagtatgcgatcacgccggaaatcgcgacgcgcttggagtaccaatggactaacaacattggcgacgcgcacacgattggcactcgtcctgacaacggcatcccaggt BBa_G0002_sequence 1 tactagag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z