BBa_K1890010
1
BBa_K1890010
mCerulean with strong consitutive promoter and RBS
2016-10-06T11:00:00Z
2016-10-16T01:24:43Z
Derived from Aequorea Victoria
mCerulean is a cyan fluorescent protein derived from ECFP. It presents a series of mutations that increase its extinction coefficient, quantum yield and fluorescence lifetime. We expressed it under the control of the strong constitutive promoter J23100 and RBS B0034.
false
false
_2355_
29445
29445
9
true
Codon optimized for E. coli.
false
Lycka Kamoen, Maria Vazquez
component2498207
1
BBa_B0030
component2498205
1
BBa_J23100
component2498209
1
BBa_K1890054
annotation2498205
1
BBa_J23100
range2498205
1
1
35
annotation2498207
1
BBa_B0030
range2498207
1
44
58
annotation2498209
1
BBa_K1890054
range2498209
1
65
784
BBa_B0030
1
BBa_B0030
RBS.1 (strong) -- modified from R. Weiss
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_44_46_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation7025
1
BBa_B0030
range7025
1
1
15
annotation1702
1
RBS
range1702
1
8
12
annotation1701
1
RBS-1\Strong
range1701
1
1
15
BBa_K1890054
1
BBa_K1890054
mCerulean
2016-10-11T11:00:00Z
2016-10-18T01:23:21Z
Derived from Aequorea Victoria
mCerulean is a cyan fluorescent protein derived from ECFP. It presents a series of mutations that increase its extinction coefficient, quantum yield and fluorescence lifetime.
false
false
_2355_
29445
29445
9
false
false
Lycka Kamoen, Maria Vazquez
BBa_J23100
1
BBa_J23100
constitutive promoter family member
2006-08-03T11:00:00Z
2015-08-31T04:08:40Z
Isolated from library of promoters
Released HQ 2013
Replace later
false
true
_52_
0
483
95
In stock
true
N/A
true
John Anderson
BBa_J23100_sequence
1
ttgacggctagctcagtcctaggtacagtgctagc
BBa_K1890054_sequence
1
atggtgagcaagggcgaggagttatttaccggggttgtaccgatcttggtcgaactggatggtgacgtaaacggccacaagttcagtgtgtcgggagaaggtgagggggatgcgacttatggtaagctgaccttaaagttcatttgcactacgggtaaattgcccgtgccttggcccacgcttgttacaacacttacctggggtgtgcaatgcttcgcacgctacccagatcacatgaaacagcatgattttttcaaaagtgctatgccagagggttatgtgcaggaacgtaccattttttttaaagatgacggtaattacaagacacgcgcagaggtgaagtttgagggggacacccttgtcaaccgtatcgagcttaaaggtattgacttcaaagaagacggcaacattcttgggcacaaattggagtacaacgcgatttctgacaacgtgtatattactgccgataaacaaaagaatggaattaaggcgaactttaaaattcgtcataatatcgaggatggttccgtgcagttggctgatcactaccaacaaaacaccccaattggggacggtccggttctgttacccgacaaccactacctgagcactcaatcgaaattgtcgaaagacccgaacgagaaacgcgatcacatggtattattggagttcgtcacggctgccggtatcaccttagggatggatgaattatacaaataa
BBa_B0030_sequence
1
attaaagaggagaaa
BBa_K1890010_sequence
1
ttgacggctagctcagtcctaggtacagtgctagctactagagattaaagaggagaaatactagatggtgagcaagggcgaggagttatttaccggggttgtacccatcttggtcgaactggatggtgacgtaaacggccacaagttcagtgtgtcgggagaaggtgagggggatgcgacttatggtaagctgaccttaaagttcatttgcactacgggtaaattgcccgtgccttggcccacgcttgttacaacacttacctggggtgtgcaatgcttcgcacgctacccagatcacatgaaacagcatgattttttcaaaagtgctatgccagagggttatgtgcaggaacgtaccattttttttaaagatgacggtaattacaagacacgcgcagaggtgaagtttgagggggacacccttgtcaaccgtatcgagcttaaaggtattgacttcaaagaagacggcaacattcttgggcacaaattggagtacaacgcgatttctgacaacgtgtatattactgccgataaacaaaagaatggaattaaggcgaactttaaaattcgtcataatatcgaggatggttccgtgcagttggctgatcactaccaacaaaacaccccaattggggacggtccggttctgttacccgacaaccactacctgagcactcaatcgaaattgtcgaaagacccgaacgagaaacgcgatcacatggtattattggagttcgtcacggctgccggtatcaccttagggatggatgaattatacaaataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z