BBa_B0030
1
BBa_B0030
RBS.1 (strong) -- modified from R. Weiss
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_44_46_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation7025
1
BBa_B0030
range7025
1
1
15
annotation1702
1
RBS
range1702
1
8
12
annotation1701
1
RBS-1\Strong
range1701
1
1
15
BBa_K1890011
1
BBa_K1890011
mVenus with strong consitutive promoter and RBS
2016-10-06T11:00:00Z
2016-10-16T01:25:25Z
Derived from Aequorea Victoria
mVenus is a variant of yellow fluorescent proteins (YFP) which contains several mutations that make it more stable1 and provide an improved speed and efficiency of maturation. We expressed it under the control of the strong constitutive promoter J23100 and RBS B0034, both sequences were available in the iGEM registry.
false
false
_2355_
29445
29445
9
false
Codon optimized for E. coli.
false
Lycka Kamoen, Maria Vazquez
component2498210
1
BBa_J23100
component2498212
1
BBa_B0030
component2498214
1
BBa_K1890055
annotation2498210
1
BBa_J23100
range2498210
1
1
35
annotation2498212
1
BBa_B0030
range2498212
1
44
58
annotation2498214
1
BBa_K1890055
range2498214
1
65
784
BBa_J23100
1
BBa_J23100
constitutive promoter family member
2006-08-03T11:00:00Z
2015-08-31T04:08:40Z
Isolated from library of promoters
Released HQ 2013
Replace later
false
true
_52_
0
483
95
In stock
true
N/A
true
John Anderson
BBa_K1890055
1
BBa_K1890055
mVenus
2016-10-11T11:00:00Z
2016-10-18T01:23:35Z
Derived from Aequorea Victoria
mVenus is a variant of yellow fluorescent protein (YFP) which contains several mutations that make it more stable and provide an improved speed and efficiency of maturation.
false
false
_2355_
29445
29445
9
false
false
Lycka Kamoen, Maria Vazquez
BBa_J23100_sequence
1
ttgacggctagctcagtcctaggtacagtgctagc
BBa_K1890011_sequence
1
ttgacggctagctcagtcctaggtacagtgctagctactagagattaaagaggagaaatactagatggttagtaaaggggaggaactgtttacaggtgtcgttccaattcttgtagaattagatggtgacgtaaacgggcataaattttcagtaagcggcgagggcgagggggatgcgacatatggcaaactgactttaaaattgatttgcacgacaggaaagctgccagtcccttggcctactctggtaactacgcttggctacggtctgcaatgtttcgcacgttatcccgaccatatgaagcaacatgatttttttaaatcagcaatgcccgaaggctacgtgcaggaacgtacaatctttttcaaagacgacggaaactataagacacgtgctgaagtaaagttcgagggagacaccttggtcaaccgtattgagttgaagggaatcgattttaaggaggacgggaacattctgggtcacaaattagagtacaattataacagccataacgtctatatcaccgcagacaaacaaaaaaacggaattaaagctaattttaagatccgtcataacattgaagatggtggtgtgcagctggccgaccattatcagcagaacacccctattggtgatggacccgttttacttccggacaaccactacctgtcataccagtcaaagttatccaaagatccgaatgagaagcgcgatcatatggtccttctggagtttgttacagccgctgggattaccttaggcatggacgaactgtacaaataa
BBa_K1890055_sequence
1
atggttagtaaaggggaggaactgtttacaggtgtcgttccaattcttgtagaattagatggtgacgtaaacgggcataaattttcagtaagcggcgagggcgagggggatgcgacatatggcaaactgactttaaaattgatttgcacgacaggaaagctgccagtcccttggcctactctggtaactacgcttggctacggtctgcaatgtttcgcacgttatcccgaccatatgaagcaacatgatttttttaaatcagcaatgcccgaaggctacgtgcaggaacgtacaatctttttcaaagacgacggaaactataagacacgtgctgaagtaaagttcgagggagacaccttggtcaaccgtattgagttgaagggaatcgattttaaggaggacgggaacattctgggtcacaaattagagtacaattataacagccataacgtctatatcaccgcagacaaacaaaaaaacggaattaaagctaattttaagatccgtcataacattgaagatggtggtgtgcagctggccgaccattatcagcagaacacccctattggtgatggacccgttttacttccggacaaccactacctgtcataccagtcaaagttatccaaagatccgaatgagaagcgcgatcatatggtccttctggagtttgttacagccgctgggattaccttaggcatggacgaactgtacaaataa
BBa_B0030_sequence
1
attaaagaggagaaa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z