LOCUS       BBa_K1890023             913 bp    DNA     linear       22-Dec-2024
DEFINITION  GFP with weak constitutive promoter, RBS and terminator
ACCESSION   BBa_K1890023
VERSION     BBa_K1890023.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23117
     RBS             36..50
                     /label=RBS-1\Strong
     RBS             43..47
                     /label=RBS
     misc_feature    36..50
                     /label=BBa_B0030
     RBS             36..50
                     /label=BBa_B0030
     CDS             57..776
                     /label=GFP protein
     CDS             57..776
                     /label=BBa_E0040
     stem_loop       796..839
                     /label=stem_loop
     misc_feature    785..864
                     /label=BBa_B0010
     terminator      785..864
                     /label=BBa_B0010
     stem_loop       880..899
                     /label=T7 TE
     misc_feature    906..906
                     /label=stop
     polyA_site      900..913
                     /label=polya
     misc_feature    873..913
                     /label=BBa_B0012
     terminator      873..913
                     /label=BBa_B0012
     misc_feature    36..913
                     /label=BBa_E0840
ORIGIN
        1 ttgacagcta gctcagtcct agggattgtg ctagcattaa agaggagaaa tactagatgc
       61 gtaaaggaga agaacttttc actggagttg tcccaattct tgttgaatta gatggtgatg
      121 ttaatgggca caaattttct gtcagtggag agggtgaagg tgatgcaaca tacggaaaac
      181 ttacccttaa atttatttgc actactggaa aactacctgt tccatggcca acacttgtca
      241 ctactttcgg ttatggtgtt caatgctttg cgagataccc agatcatatg aaacagcatg
      301 actttttcaa gagtgccatg cccgaaggtt atgtacagga aagaactata tttttcaaag
      361 atgacgggaa ctacaagaca cgtgctgaag tcaagtttga aggtgatacc cttgttaata
      421 gaatcgagtt aaaaggtatt gattttaaag aagatggaaa cattcttgga cacaaattgg
      481 aatacaacta taactcacac aatgtataca tcatggcaga caaacaaaag aatggaatca
      541 aagttaactt caaaattaga cacaacattg aagatggaag cgttcaacta gcagaccatt
      601 atcaacaaaa tactccaatt ggcgatggcc ctgtcctttt accagacaac cattacctgt
      661 ccacacaatc tgccctttcg aaagatccca acgaaaagag agaccacatg gtccttcttg
      721 agtttgtaac agctgctggg attacacatg gcatggatga actatacaaa taataatact
      781 agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc
      841 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct
      901 ttctgcgttt ata
//