BBa_K1891000 1 BBa_K1891000 alpha-tubulin 2016-10-09T11:00:00Z 2016-10-10T01:36:56Z We get this gene from human breast cancer cell line Mcf-7 cDNA using PCR. It encodes alpha-tubulin from human breast cancer cell line Mcf-7. We cloned this gene to an expression plasmid pET30a(+) and add IPTG until its final concentration is 1mmol/L, incubate for 4-6h(37??C, 250rpm) to induce the expression of alpha-tubulin false false _2356_ 27040 27040 9 false To get this part, we extracted human breast cancer cell line Mcf-7 cDNA. And then we designed primers to get this gene fragment with prefix and suffix. Because the sequence of alpha -tubulin have tow EcoRI restriction enzyme cutting sites. So we use point mutation to mutate these sites. false Zhao Jingyu annotation2492396 1 alpha-tubulin range2492396 1 1 1356 BBa_K1891000_sequence 1 atgcgtgaatgcattagcattcacgtgggccaggccggtgtgcaaatcggtaatgcctgctgggagctgtattgtctggagcatggcatccagccggatggccagatgcctagcgacaaaaccattggcggcggcgatgatagtttcaataccttttttagcgagacaggtgccggcaagcatgtgccgcgtgccgtttttgtggatctggaaccgaccgtgatcgatgaagttcgcacaggtacctaccgtcagctgttccatccggagcagctgattaccggcaaagaggacgccgccaataactatgcacgcggccactacaccattggcaaagaaattattgatttagtgttagatcgtattcgcaagctggccgatcagtgtaccggcttacaaggctttctggtgtttcatagctttggcggcggtacaggtagcggctttaccagcctgttaatggaacgcctgagcgtggactacggcaagaaaagcaaactggaatttagtatttatccggccccgcaggtgagcaccgcagtggtggaaccttataacagcatcctgaccacccataccaccctggagcatagcgattgcgcatttatggtggacaacgaggcaatctatgacatctgccgccgcaatctggatattgagcgcccgacctacaccaacctgaaccgcctgattggccagatcgtgagcagcatcaccgccagtctgcgcttcgacggtgccctgaatgtggacctgaccgagtttcagaccaacctggttccgtatccgcgcattcatttccctctggccacctacgccccggtgattagtgccgaaaaagcctaccacgagcagctgagcgtggcagaaattaccaacgcctgcttcgagcctgcaaatcagatggtgaagtgcgatccgcgtcacggcaaatatatggcatgctgtctgctgtatcgcggcgatgttgtgccgaaagatgtgaatgccgccattgccaccatcaagaccaagcgtaccatccagtttgtggattggtgcccgaccggcttcaaagtgggcattaattaccagccgccgaccgttgttccgggcggtgatctggcaaaagttcagcgtgccgtgtgcatgctgagcaatacaaccgcaatcgccgaagcctgggcacgcctggaccacaagttcgacctgatgtatgccaagcgtgccttcgtgcattggtacgttggcgaaggtatggaggaaggcgagtttagcgaagcccgcgaagatatggccgcactggaaaaggactacgaggaagtgggcgtggatagcgttgaaggtgaaggcgaggaagaaggcgaagagtattaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z