BBa_K1891001 1 BBa_K1891001 beta-tubulin 2016-10-09T11:00:00Z 2016-10-10T01:46:35Z We get this gene from human breast cancer cell line Mcf-7 cDNA using PCR. It encodes beta-tubulin from human breast cancer cell line Mcf-7. We cloned this gene to an expression plasmid pET30a(+) and add IPTG until its final concentration is 1mmol/L, incubate for 4-6h(37??C, 250rpm) to induce the expression of beta-tubulin. false false _2356_ 27040 27040 9 false To get this part, we extracted human breast cancer cell line Mcf-7 cDNA. And then we designed primers to get this gene fragment with prefix and suffix. Because the sequence of beta -tubulin has a PstI restriction enzyme cutting site. So we use point mutation to mutate these sites. false Zhao Jingyu annotation2492413 1 beta-tubulin range2492413 1 1 1335 BBa_K1891001_sequence 1 atgcgtgaaatcgtgcacattcaggcaggccagtgcggtaaccagatcggcgccaagttttgggaggttatcagcgacgagcacggcatcgatccgaccggtacctatcatggcgacagtgatttacaactggaccgtatcagcgtgtattacaacgaggccaccggcggtaaatacgtgccgcgtgccattctggtggatctggaaccgggtaccatggatagtgttcgtagcggcccgtttggtcagatctttcgcccggataactttgtgttcggccagagcggtgccggcaataactgggccaaaggccactatactgagggcgcagagctggtggatagcgtgctggacgtggtgcgcaaagaagccgaaagttgtgattgcttacaaggctttcagctgacccacagtctgggtggcggtaccggtagtggtatgggtaccctgctgatcagcaaaatccgcgaggaatacccggaccgcattatgaacaccttcagcgtggttccgagtccgaaagtgagcgacaccgtggttgaaccttacaatgccaccctgagcgtgcaccagctggttgagaataccgatgaaacctattgtatcgataatgaggccttatatgacatttgctttcgcacactgaagctgaccacccctacatatggcgatctgaaccatctggtgagcgcaaccatgagcggtgtgacaacctgtctgcgctttcctggccagctgaacgccgatttacgcaaactggccgtgaatatggttccgtttcctcgtctgcatttctttatgccgggctttgccccgttaaccagtcgcggtagtcagcagtatcgcgcactgaccgttccggaactgacccagcaagtgttcgatgccaagaacatgatggcagcatgcgatccgcgtcatggccgctatctgaccgtggccgccgtttttcgtggtcgtatgagcatgaaggaagtggacgagcagatgctgaacgttcagaacaaaaacagcagctacttcgtggaatggatcccgaacaacgttaagaccgcagtgtgtgatatcccgccgcgcggtctgaaaatggccgtgacctttatcggcaacagcaccgccatccaggaattattcaaacgcattagcgaacagttcaccgccatgtttcgccgtaaagccttcctgcactggtataccggtgaaggcatggacgagatggagtttaccgaggccgagagtaacatgaatgacctggtgagcgagtaccagcagtatcaggatgccacagccgaggaagaagaagatttcggcgaagaagccgaagaggaagcataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z