BBa_K1891004 1 BBa_K1891004 C-Luciferase 2016-10-09T11:00:00Z 2016-10-10T04:27:40Z We get this gene from pCambia1300-C-Luciferase plasmid. It encodes C-terminal of luciferase. When it meets N-terminal of luciferase, these two parts can become a complete luciferase. We cloned this gene to an expression plasmid pET30a(+) and add IPTG until its final concentration is 1mmol/L, incubate for 4-6h(37??C, 250rpm) to induce the expression of N-Luciferase. We use it as a part of reporter gene to detect whether it has taxol or its analogues. false false _2356_ 27040 27040 9 false To get this part, we designed primers according to the gene sequence and then cloned it to our expression vector.Because this gene doesn't have a initiation codon, so we designed a initiation codon in its forward primer. false Zhao Jingyu annotation2492415 1 C-Luciferase range2492415 1 1 459 BBa_K1891004_sequence 1 atgtccggttatgtaaacaatccggaagcgaccaacgccttgattgacaaggatggatggctacattctggagacatagcttactgggacgaagacgaacacttcttcatcgttgaccgcctgaagtctctgattaagtacaaaggctatcaggtggctcccgctgaattggaatccatcttgctccaacaccccaacatcttcgacgcaggtgtcgcaggtcttcccgacgatgacgccggtgaacttcccgccgccgttgttgttttggagcacggaaagacgatgacggaaaaagagatcgtggattacgtcgccagtcaagtaacaaccgcgaaaaagttgcgcggaggagttgtgtttgtggacgaagtaccgaaaggtcttaccggaaaactcgacgcaagaaaaatcagagagatcctcataaaggccaagaagggcggaaagatcgccgtg igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z