BBa_K1891006
1
BBa_K1891006
C-Luciferase+alpha-tubulin
2016-10-09T11:00:00Z
2016-10-10T09:27:47Z
C-Luciferase comes from pCambia1300-C-Luciferase plasmid. Alpha-tubulin comes from human breast cancer cell line Mcf-7 cDNA.
It expresses C-Luciferase-alpha-tubulin fusion protein. The C-terminal of C-Luciferase links to the N-terminal of alpha-tubulin. We connected this two gene fragment using fusion PCR. And then we cloned it into pET30a(+) and add IPTG until its final concentration is 1mmol/L, incubate for 4-6h(37??C, 250rpm) to induce the expression of this fusion protein.
false
false
_2356_
27040
27040
9
false
We connected this two gene fragments using fusion PCR. First we acquired alpha-tubulin gene fragment and C-Luciferase gene fragment. And then we redesigned a antisense primer of C-Luciferase and a sense primer of alpha-tubulin with a linker about 42bp. And then doing fusion PCR.
false
Zhao Jingyu
annotation2492756
1
linker
range2492756
1
459
501
annotation2492752
1
C-luciferase
range2492752
1
1
459
annotation2492757
1
alpha-tubulin
range2492757
1
502
1857
BBa_K1891006_sequence
1
atgtccggttatgtaaacaatccggaagcgaccaacgccttgattgacaaggatggatggctacattctggagacatagcttactgggacgaagacgaacacttcttcatcgttgaccgcctgaagtctctgattaagtacaaaggctatcaggtggctcccgctgaattggaatccatcttgctccaacaccccaacatcttcgacgcaggtgtcgcaggtcttcccgacgatgacgccggtgaacttcccgccgccgttgttgttttggagcacggaaagacgatgacggaaaaagagatcgtggattacgtcgccagtcaagtaacaaccgcgaaaaagttgcgcggaggagttgtgtttgtggacgaagtaccgaaaggtcttaccggaaaactcgacgcaagaaaaatcagagagatcctcataaaggccaagaagggcggaaagatcgccgtggctaccaccaccgctaccaccaccaccgctaccaccaccaccatgcgtgaatgcattagcattcacgtgggccaggccggtgtgcaaatcggtaatgcctgctgggagctgtattgtctggagcatggcatccagccggatggccagatgcctagcgacaaaaccattggcggcggcgatgatagtttcaataccttttttagcgagacaggtgccggcaagcatgtgccgcgtgccgtttttgtggatctggaaccgaccgtgatcgatgaagttcgcacaggtacctaccgtcagctgttccatccggagcagctgattaccggcaaagaggacgccgccaataactatgcacgcggccactacaccattggcaaagaaattattgatttagtgttagatcgtattcgcaagctggccgatcagtgtaccggcttacaaggctttctggtgtttcatagctttggcggcggtacaggtagcggctttaccagcctgttaatggaacgcctgagcgtggactacggcaagaaaagcaaactggaatttagtatttatccggccccgcaggtgagcaccgcagtggtggaaccttataacagcatcctgaccacccataccaccctggagcatagcgattgcgcatttatggtggacaacgaggcaatctatgacatctgccgccgcaatctggatattgagcgcccgacctacaccaacctgaaccgcctgattggccagatcgtgagcagcatcaccgccagtctgcgcttcgacggtgccctgaatgtggacctgaccgagtttcagaccaacctggttccgtatccgcgcattcatttccctctggccacctacgccccggtgattagtgccgaaaaagcctaccacgagcagctgagcgtggcagaaattaccaacgcctgcttcgagcctgcaaatcagatggtgaagtgcgatccgcgtcacggcaaatatatggcatgctgtctgctgtatcgcggcgatgttgtgccgaaagatgtgaatgccgccattgccaccatcaagaccaagcgtaccatccagtttgtggattggtgcccgaccggcttcaaagtgggcattaattaccagccgccgaccgttgttccgggcggtgatctggcaaaagttcagcgtgccgtgtgcatgctgagcaatacaaccgcaatcgccgaagcctgggcacgcctggaccacaagttcgacctgatgtatgccaagcgtgccttcgtgcattggtacgttggcgaaggtatggaggaaggcgagtttagcgaagcccgcgaagatatggccgcactggaaaaggactacgaggaagtgggcgtggatagcgttgaaggtgaaggcgaggaagaaggcgaagagtattaa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z