BBa_K1891008 1 BBa_K1891008 alpha-tubulin+YCE 2016-10-09T11:00:00Z 2016-10-10T10:32:28Z Alpha-tubulin comes from human breast cancer cell line Mcf-7. YCE comes from pSPYCE plasmid. This part expresses alpha-tubulin-YCE fusion protein. The C-terminal of alpha-tubulin links to the N-terminal of YCE. We connected this two gene fragment using fusion PCR. And then we cloned it into pET30a(+) and add IPTG until its final concentration is 1mmol/L, incubate for 4-6h(37??C, 250rpm) to induce the expression of this fusion protein. false false _2356_ 27040 27040 9 false We connected this two gene fragments using fusion PCR. First we acquired alpha-tubulin gene fragment and YCE gene fragment. And then we redesigned a antisense primer ofalpha-tubulin and a sense primer of YCE with a linker about 42bp. And then doing fusion PCR. false Zhao Jingyu annotation2492762 1 YCE range2492762 1 1399 1650 annotation2492761 1 alpha-tubulin range2492761 1 1 1356 annotation2492764 1 linker range2492764 1 1357 1398 BBa_K1891008_sequence 1 atgcgtgaatgcattagcattcacgtgggccaggccggtgtgcaaatcggtaatgcctgctgggagctgtattgtctggagcatggcatccagccggatggccagatgcctagcgacaaaaccattggcggcggcgatgatagtttcaataccttttttagcgagacaggtgccggcaagcatgtgccgcgtgccgtttttgtggatctggaaccgaccgtgatcgatgaagttcgcacaggtacctaccgtcagctgttccatccggagcagctgattaccggcaaagaggacgccgccaataactatgcacgcggccactacaccattggcaaagaaattattgatttagtgttagatcgtattcgcaagctggccgatcagtgtaccggcttacaaggctttctggtgtttcatagctttggcggcggtacaggtagcggctttaccagcctgttaatggaacgcctgagcgtggactacggcaagaaaagcaaactggaatttagtatttatccggccccgcaggtgagcaccgcagtggtggaaccttataacagcatcctgaccacccataccaccctggagcatagcgattgcgcatttatggtggacaacgaggcaatctatgacatctgccgccgcaatctggatattgagcgcccgacctacaccaacctgaaccgcctgattggccagatcgtgagcagcatcaccgccagtctgcgcttcgacggtgccctgaatgtggacctgaccgagtttcagaccaacctggttccgtatccgcgcattcatttccctctggccacctacgccccggtgattagtgccgaaaaagcctaccacgagcagctgagcgtggcagaaattaccaacgcctgcttcgagcctgcaaatcagatggtgaagtgcgatccgcgtcacggcaaatatatggcatgctgtctgctgtatcgcggcgatgttgtgccgaaagatgtgaatgccgccattgccaccatcaagaccaagcgtaccatccagtttgtggattggtgcccgaccggcttcaaagtgggcattaattaccagccgccgaccgttgttccgggcggtgatctggcaaaagttcagcgtgccgtgtgcatgctgagcaatacaaccgcaatcgccgaagcctgggcacgcctggaccacaagttcgacctgatgtatgccaagcgtgccttcgtgcattggtacgttggcgaaggtatggaggaaggcgagtttagcgaagcccgcgaagatatggccgcactggaaaaggactacgaggaagtgggcgtggatagcgttgaaggtgaaggcgaggaagaaggcgaagagtattaagctaccaccaccgctaccaccaccaccgctaccaccaccaccaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z