LOCUS BBa_K189101 9626 bp DNA linear 23-Nov-2024 DEFINITION pET linkage group of GenSniper via Bottom Up approach ACCESSION BBa_K189101 VERSION BBa_K189101.1 FEATURES Location/Qualifiers ORIGIN 1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa 61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt 121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt 181 cgacggagct cgaattcaat gcgtcgagac gacagtgaga agaggaggag acaactaaca 241 gcgtcgggcc taagtttacg acgtcggcgg gtccgcccgc caaattctgg ccgacgtgcc 301 gcgaggtagc aaagtgcctg gacgacccgt ttttaaagga ctatcagcag tggcgcaaaa 361 cgcgtgagaa agagcatcca tgagtcaggc cgaagatagt cgtagtggcg aaggacttga 421 agaaagtctg gtagctaccg gtatgctggc ctcggatagg tcagcgtcaa gggggtccgt 481 gacgcccgaa ggacttttga cttcgcgcga aaacttccat tgcagtggtg cgccgcttgc 541 taccggagaa ggtcggtcgt gtctttgtag accgagcgga ctgccctacg ctgcttaaaa 601 gcggcggggt atttcatgcg ggtgctgagc aagcgtgacc gggcacggca cctcatcgag 661 tagacccgca ttaaggccct ttcgacgagt atgctctgtg ggtcgggccg tcgctatatg 721 gcgtcgtcac tgacgagttt gtgcctcatc ggcaataggc ataggactcg gcagacgtcc 781 aagtcactca gtgggccgta gtccacgcca tgaaaacgcg gagggtcggc ctggccgcga 841 cgccgcatta tgcgccgtta aagtggttag gtcggccagt cggaaagggc gacgaggact 901 gacaagcgcg ggtcttattt taggtagcga ctgacgcata ggtcgagtga gagttaccac 961 cgccgtatgt agcggaagtg ttaccgcgag acgtcgacgc acaagacgtc gcacagctcg 1021 tagaagtaga cgaggtagtg cgacattttg tgtaaacgtg gcgctcagac gggcaggagg 1081 tgcccaagtt tttgcactta cttgctccgc gcgggcggcc cattgagtgc cccataggta 1141 caggtaaaga cgccgtaggt cggtcctatg ggcaggagcg actgcattat agggtcgcgg 1201 cgtggcgaca gtaattagac gtgtggccgt gccgtcaagg ccgacagcgg ccataacaag 1261 cccaacgact acgcgaagcc cgactggtag gccttgacac aggccttttc ggcgctgctt 1321 gaccataggg tccaccggac ttgcttgtca agtggcaatt tccgcacgta ccggtgtgga 1381 agggcttagt agtaccattt gcacgcaaaa gcgagttgca gttacgtcgt cgtcagtagg 1441 agccgtttga gaaaggtacg gcgaagttgg agcgcccttt tccgtgcccg aagaaggagg 1501 ggctacgggt ctatcgcggt cgaacccgct actgactcgg ccttttttct gggctgctat 1561 actaggacta cgtcgaccta ccgcaaccgc cgtatcggca ataacgcatg gtctagcaga 1621 cgcgcccgta acggtgccca tttcaacccg ttgtcccgac gtaggtgtga aagtgagcca 1681 cccaaggtgc tggcgttgac gggaggttta ggcgacggtg gcggcactat tggccgtata 1741 agcgcgtcgc tacagtacgg caggccgggg tcttcccacc cttaccaccc gcaaaagtat 1801 gtattttagg acgtccaggg gacgcagcga cacagtacgg ccagacgtga aggtcgagac 1861 gttatataaa aaagtccagt ctgtgccttc accggcattt gaggtgggaa gcaggcagaa 1921 agacatgaca acggtgggca aatggacagt agtccagtac gtcacggcgt gcccgtcgcc 1981 gttcaagaag gacagcgcag tagatcatag taagtaggag aggcctattc cgtgcccgca 2041 ttagacggtc acaaaagaac aaccaacgac gtggtaggag aaggacgtcc gagcggtcgt 2101 cgcgtgactc taggtcgacg gtcgcccttt atgactacgc gtcgcggcgg tcgcgtattt 2161 gcttcgtcag ctcacggagt aacgcagcga aaaacgacag ggtgtcataa aaaaaggacg 2221 gtaggtgggt aaaaagctgg acgagaagtc gtcagtcgac gacgcgaagc cagtctagtt 2281 tttataggcc caataagccc ttcacttgcc gtggcccttc gccaagtagg ggaaggccgc 2341 agtcacactt cgccaatatt tagacgagaa agcgccatag gcatggctaa agccattcca 2401 tttggggcaa aaacaaagcg aatgcaccgt acgaccggtg gccgaaaggc atctgcctac 2461 ggggaaatta cccctagtgg gccttgtcgg gtacaaaaaa gctcgcaagt atgtgttacc 2521 agcccagtta gggcggtcat agggtcgtct atgccctata gctgtaaaga cgtggtaagg 2581 ccgcccatat ccaaaataac taccggagta ggtgtgcgtc gtcgcagaca agtagcagca 2641 ccgccgggta ttattagacg gccagttagt cggtcgaaag gagtgggccg ggggtagggg 2701 tatgcgcgta aagcatcgcc aggtcgaccc tcagctatgg ccgccagtcc attcggtgtg 2761 ccagtccttg cccgcgactt attacgagaa aggcgagacg gtagtgaagt cgtaggcctg 2821 caagcggtta aaagcggagg gtgcagagtg gctcgcacca caaatgcttc caaaatgcaa 2881 aagggcatag gggaaagcaa aagtaggtca gaaactgtta gacgtgggtc caccacttgc 2941 ccgacatgcg acaggtctac actttccagt gtgacagtcc accgagttag agaagtggcc 3001 tactgctttt ggtctcttac ggtagtgccc aggtctaggg ccagaaaagc gtctatattg 3061 cccgtagtca tttcaggtcg aggacgaccg cctactgcgt ccgtaatacg agcgtctcta 3121 ttttgtgcga cctccccagt aggccgcagg taaactccgg tttgccgcag agaaacagcg 3181 gtttaaattc tatgacgagg aggggcgtta cgcccgtccg ttgtactttt gcgtatttta 3241 cgcccctaag tgaccgacgt gcgagttaga ctgtccacgg agagtgaaaa ccgcacctcg 3301 gtgcctacct gaaaccggtc tggctcggaa gttatgcgaa cagtgggtcc ttgcagcctc 3361 tcggaaggac aagttatagt agtagttttc gtcgttcaag tagtattggg cggtgtaggt 3421 ggctgaaaag tgccatcaaa aaacggcgaa atggcgggtc cgtggtcttc ggtgcgggta 3481 atcactttgc gaagtaccac tcgcacaata gggccacgaa aaacggtatg gtgccccggt 3541 cgcggtcgtc gctgccttat agtgcttatc agccgagttg cacccaaaag tatttcaaga 3601 gccgtagtgg taggcagccg ttggtctatt cccacaacgc gacgaatacg agatatttca 3661 tccgtatttg tgggtcgtcg taaaacctta ttggctgtgc ccgtctgaag tggtgtaagt 3721 ggagtgccta catcagcgac gggtagcgta agtactaccg ggcgactttc ccgtcacaaa 3781 gggtcgcggg aaggaccata cgcctaagaa agccctctat cattaatcgt aggcgggtaa 3841 gttgccgcca gacaccgagg ccggacttgt cactcgcttc gggccgcgcc tgttttacgg 3901 cgtcggacaa ttggactgac aagctatata agtgagtcgt tggggccata gtcaagtagg 3961 tcgcgccgac gaaacaagta ccgaaactac tatagggcaa agtcctttag ttgtacagcc 4021 aaaaggtcaa ggccttttgc ggcgacgtgg ctgtccccct cgggcagctc ttatgaccgt 4081 taaagtggac gctaggcgct gtcgtgcttt catgtcttac gccaaaggtg gtgaagtcgc 4141 ctcagagacc gtaagaagtc aaggacacgc agccggacgc gtgcgcattc agctaccgca 4201 agcatgagtt atcaaggacc gacctctaga cggagcgacc ggacggcgtc aagaagttgg 4261 agggccgcgt cgaaaagcaa gagttaaagt cgtagggaaa gccgtatggt aaaatactgc 4321 cgccgtctca gtatttcgtg gagtaatggg aacggtggcg gagcgtcttg cccgtaaggg 4381 acaaggacgg tcaagactta ccatgcctat gagcgtggct tttacagtcg gtcgacgaaa 4441 aacaactgaa ggtaggatcc gcgacccatt tgctgtccac cagtcatgct agccatatgg 4501 ctgccgcgcg gcaccaggcc gctgctgtga tgatgatgat gatggctgct gcccatggta 4561 tatctccttc ttaaagttaa acaaaattat ttctagaggg gaattgttat ccgctcacaa 4621 ttcccctata gtgagtcgta ttaatttcgc gggatcgaga tctcgatcct ctacgccgga 4681 cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg ctggcgccta tatcgccgac 4741 atcaccgatg gggaagatcg ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg 4801 ggtatggtgg caggccccgt ggccggggga ctgttgggcg ccatctcctt gcatgcacca 4861 ttccttgcgg cggcggtgct caacggcctc aacctactac tgggctgctt cctaatgcag 4921 gagtcgcata agggagagcg tcgagatccc ggacaccatc gaatggcgca aaacctttcg 4981 cggtatggca tgatagcgcc cggaagagag tcaattcagg gtggtgaatg tgaaaccagt 5041 aacgttatac gatgtcgcag agtatgccgg tgtctcttat cagaccgttt cccgcgtggt 5101 gaaccaggcc agccacgttt ctgcgaaaac gcgggaaaaa gtggaagcgg cgatggcgga 5161 gctgaattac attcccaacc gcgtggcaca acaactggcg ggcaaacagt cgttgctgat 5221 tggcgttgcc acctccagtc tggccctgca cgcgccgtcg caaattgtcg cggcgattaa 5281 atctcgcgcc gatcaactgg gtgccagcgt ggtggtgtcg atggtagaac gaagcggcgt 5341 cgaagcctgt aaagcggcgg tgcacaatct tctcgcgcaa cgcgtcagtg ggctgatcat 5401 taactatccg ctggatgacc aggatgccat tgctgtggaa gctgcctgca ctaatgttcc 5461 ggcgttattt cttgatgtct ctgaccagac acccatcaac agtattattt tctcccatga 5521 agacggtacg cgactgggcg tggagcatct ggtcgcattg ggtcaccagc aaatcgcgct 5581 gttagcgggc ccattaagtt ctgtctcggc gcgtctgcgt ctggctggct ggcataaata 5641 tctcactcgc aatcaaattc agccgatagc ggaacgggaa ggcgactgga gtgccatgtc 5701 cggttttcaa caaaccatgc aaatgctgaa tgagggcatc gttcccactg cgatgctggt 5761 tgccaacgat cagatggcgc tgggcgcaat gcgcgccatt accgagtccg ggctgcgcgt 5821 tggtgcggat atctcggtag tgggatacga cgataccgaa gacagctcat gttatatccc 5881 gccgttaacc accatcaaac aggattttcg cctgctgggg caaaccagcg tggaccgctt 5941 gctgcaactc tctcagggcc aggcggtgaa gggcaatcag ctgttgcccg tctcactggt 6001 gaaaagaaaa accaccctgg cgcccaatac gcaaaccgcc tctccccgcg cgttggccga 6061 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg 6121 caattaatgt aagttagctc actcattagg caccgggatc tcgaccgatg cccttgagag 6181 ccttcaaccc agtcagctcc ttccggtggg cgcggggcat gactatcgtc gccgcactta 6241 tgactgtctt ctttatcatg caactcgtag gacaggtgcc ggcagcgctc tgggtcattt 6301 tcggcgagga ccgctttcgc tggagcgcga cgatgatcgg cctgtcgctt gcggtattcg 6361 gaatcttgca cgccctcgct caagccttcg tcactggtcc cgccaccaaa cgtttcggcg 6421 agaagcaggc cattatcgcc ggcatggcgg ccccacgggt gcgcatgatc gtgctcctgt 6481 cgttgaggac ccggctaggc tggcggggtt gccttactgg ttagcagaat gaatcaccga 6541 tacgcgagcg aacgtgaagc gactgctgct gcaaaacgtc tgcgacctga gcaacaacat 6601 gaatggtctt cggtttccgt gtttcgtaaa gtctggaaac gcggaagtca gcgccctgca 6661 ccattatgtt ccggatctgc atcgcaggat gctgctggct accctgtgga acacctacat 6721 ctgtattaac gaagcgctgg cattgaccct gagtgatttt tctctggtcc cgccgcatcc 6781 ataccgccag ttgtttaccc tcacaacgtt ccagtaaccg ggcatgttca tcatcagtaa 6841 cccgtatcgt gagcatcctc tctcgtttca tcggtatcat tacccccatg aacagaaatc 6901 ccccttacac ggaggcatca gtgaccaaac aggaaaaaac cgcccttaac atggcccgct 6961 ttatcagaag ccagacatta acgcttctgg agaaactcaa cgagctggac gcggatgaac 7021 aggcagacat ctgtgaatcg cttcacgacc acgctgatga gctttaccgc agctgcctcg 7081 cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag 7141 cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg 7201 gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga tagcggagtg tatactggct 7261 taactatgcg gcatcagagc agattgtact gagagtgcac catatatgcg gtgtgaaata 7321 ccgcacagat gcgtaaggag aaaataccgc atcaggcgct cttccgcttc ctcgctcact 7381 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta 7441 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag 7501 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc 7561 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta 7621 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg 7681 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc 7741 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac 7801 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac 7861 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg 7921 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga 7981 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt 8041 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag 8101 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct 8161 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgaacaa taaaactgtc 8221 tgcttacata aacagtaata caaggggtgt tatgagccat attcaacggg aaacgtcttg 8281 ctctaggccg cgattaaatt ccaacatgga tgctgattta tatgggtata aatgggctcg 8341 cgataatgtc gggcaatcag gtgcgacaat ctatcgattg tatgggaagc ccgatgcgcc 8401 agagttgttt ctgaaacatg gcaaaggtag cgttgccaat gatgttacag atgagatggt 8461 cagactaaac tggctgacgg aatttatgcc tcttccgacc atcaagcatt ttatccgtac 8521 tcctgatgat gcatggttac tcaccactgc gatccccggg aaaacagcat tccaggtatt 8581 agaagaatat cctgattcag gtgaaaatat tgttgatgcg ctggcagtgt tcctgcgccg 8641 gttgcattcg attcctgttt gtaattgtcc ttttaacagc gatcgcgtat ttcgtctcgc 8701 tcaggcgcaa tcacgaatga ataacggttt ggttgatgcg agtgattttg atgacgagcg 8761 taatggctgg cctgttgaac aagtctggaa agaaatgcat aaacttttgc cattctcacc 8821 ggattcagtc gtcactcatg gtgatttctc acttgataac cttatttttg acgaggggaa 8881 attaataggt tgtattgatg ttggacgagt cggaatcgca gaccgatacc aggatcttgc 8941 catcctatgg aactgcctcg gtgagttttc tccttcatta cagaaacggc tttttcaaaa 9001 atatggtatt gataatcctg atatgaataa attgcagttt catttgatgc tcgatgagtt 9061 tttctaagaa ttaattcatg agcggataca tatttgaatg tatttagaaa aataaacaaa 9121 taggggttcc gcgcacattt ccccgaaaag tgccacctga aattgtaaac gttaatattt 9181 tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa 9241 tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag 9301 tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg 9361 tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga 9421 ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg 9481 gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg 9541 cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc 9601 cgctacaggg cgcgtcccat tcgcca //