BBa_K1891012 1 BBa_K1891012 YCE+beta-tubulin 2016-10-09T11:00:00Z 2016-10-10T11:04:44Z Beta-tubulin comes from human breast cancer cell line Mcf-7. YCE comes from pSPYCE plasmid. This part expresses YCE-beta-tubulin fusion protein. The C-terminal of YCE links to the N-terminal of YCE. We connected this two gene fragment using fusion PCR. And then we cloned it into pET30a(+) and add IPTG until its final concentration is 1mmol/L, incubate for 4-6h(37??C, 250rpm) to induce the expression of this fusion protein. false false _2356_ 27040 27040 9 false We connected this two gene fragments using fusion PCR. First we acquired YCE gene fragment and beta-tubulin gene fragment. And then we redesigned a antisense primer of YCE and a sense primer of beta-tubulin with a linker about 42bp. And then doing fusion PCR. false Zhao Jingyu annotation2492779 1 beta-tubulin range2492779 1 295 1629 annotation2492775 1 YCE range2492775 1 1 252 annotation2492778 1 linker range2492778 1 253 294 BBa_K1891012_sequence 1 aagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaagctaccaccaccgctaccaccaccaccgctaccaccaccaccatgcgtgaaatcgtgcacattcaggcaggccagtgcggtaaccagatcggcgccaagttttgggaggttatcagcgacgagcacggcatcgatccgaccggtacctatcatggcgacagtgatttacaactggaccgtatcagcgtgtattacaacgaggccaccggcggtaaatacgtgccgcgtgccattctggtggatctggaaccgggtaccatggatagtgttcgtagcggcccgtttggtcagatctttcgcccggataactttgtgttcggccagagcggtgccggcaataactgggccaaaggccactatactgagggcgcagagctggtggatagcgtgctggacgtggtgcgcaaagaagccgaaagttgtgattgcttacaaggctttcagctgacccacagtctgggtggcggtaccggtagtggtatgggtaccctgctgatcagcaaaatccgcgaggaatacccggaccgcattatgaacaccttcagcgtggttccgagtccgaaagtgagcgacaccgtggttgaaccttacaatgccaccctgagcgtgcaccagctggttgagaataccgatgaaacctattgtatcgataatgaggccttatatgacatttgctttcgcacactgaagctgaccacccctacatatggcgatctgaaccatctggtgagcgcaaccatgagcggtgtgacaacctgtctgcgctttcctggccagctgaacgccgatttacgcaaactggccgtgaatatggttccgtttcctcgtctgcatttctttatgccgggctttgccccgttaaccagtcgcggtagtcagcagtatcgcgcactgaccgttccggaactgacccagcaagtgttcgatgccaagaacatgatggcagcatgcgatccgcgtcatggccgctatctgaccgtggccgccgtttttcgtggtcgtatgagcatgaaggaagtggacgagcagatgctgaacgttcagaacaaaaacagcagctacttcgtggaatggatcccgaacaacgttaagaccgcagtgtgtgatatcccgccgcgcggtctgaaaatggccgtgacctttatcggcaacagcaccgccatccaggaattattcaaacgcattagcgaacagttcaccgccatgtttcgccgtaaagccttcctgcactggtataccggtgaaggcatggacgagatggagtttaccgaggccgagagtaacatgaatgacctggtgagcgagtaccagcagtatcaggatgccacagccgaggaagaagaagatttcggcgaagaagccgaagaggaagcataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z