BBa_K1891013 1 BBa_K1891013 beta-tubulin-C- Luciferase 2016-10-10T11:00:00Z 2016-10-11T08:03:32Z Beta-tubulin comes from human breast cancer cell line Mcf-7 cDNA. C- Luciferase comes from pCambia1300-C-Luciferase plasmid. It expresses beta-tubulin- C-Luciferase fusion protein with a 42bp linker. The C-terminal of beta-tubulin links to the N-terminal of C-Luciferase. We connected this two gene fragment using fusion PCR. And then we cloned it into pET30a(+) and add IPTG until its final concentration is 1mmol/L, incubate for 4-6h(37??C, 250rpm) to induce the expression of this fusion protein. false false _2356_ 27040 27040 9 false We connected this two gene fragments using fusion PCR. First we acquired beta-tubulin gene fragment and C-Luciferase gene fragment. And then we redesigned a antisense primer of beta-Luciferase and a sense primer of C-Luciferase with a linker about 42bp. And then doing fusion PCR. false Zhao Jingyu annotation2492385 1 C- Luciferase-beta-tubulin range2492385 1 1 1919 BBa_K1891013_sequence 1 atgtccggttatgtaaacaatccggaagcgaccaacgccttgattgacaaggatggatggctacattctggagacatagcttactgggacgaagacgaacacttcttcatcgttgaccgcctgaagtctctgattaagtacaaaggctatcaggtggctcccgctgaattggaatccatcttgctccaacaccccaacatcttcgacgcaggtgtcgcaggtcttcccgacgatgacgccggtgaacttcccgccgccgttgttgttttggagcacggaaagacgatgacggaaaaagagatcgtggattacgtcgccagtcaagtaacaaccgcgaaaaagttgcgcggaggagttgtgtttgtggacgaagtaccgaaaggtcttaccggaaaactcgacgcaagaaaaatcagagagatcctcataaaggccaagaagggcggaaagatcgccgtgacaagtttgtacaaaaaagctgaacgagaaacgtaaaatgatataaatatcaatatattaaattagattttgcataaaaaacagactacataatactgtaaaacacaacatatccagtcactatgatgcgtgaaatcgtgcacattcaggcaggccagtgcggtaaccagatcggcgccaagttttgggaggttatcagcgacgagcacggcatcgatccgaccggtacctatcatggcgacagtgatttacaactggaccgtatcagcgtgtattacaacgaggccaccggcggtaaatacgtgccgcgtgccattctggtggatctggaaccgggtaccatggatagtgttcgtagcggcccgtttggtcagatctttcgcccggataactttgtgttcggccagagcggtgccggcaataactgggccaaaggccactatactgagggcgcagagctggtggatagcgtgctggacgtggtgcgcaaagaagccgaaagttgtgattgcttacaaggctttcagctgacccacagtctgggtggcggtaccggtagtggtatgggtaccctgctgatcagcaaaatccgcgaggaatacccggaccgcattatgaacaccttcagcgtggttccgagtccgaaagtgagcgacaccgtggttgaaccttacaatgccaccctgagcgtgcaccagctggttgagaataccgatgaaacctattgtatcgataatgaggccttatatgacatttgctttcgcacactgaagctgaccacccctacatatggcgatctgaaccatctggtgagcgcaaccatgagcggtgtgacaacctgtctgcgctttcctggccagctgaacgccgatttacgcaaactggccgtgaatatggttccgtttcctcgtctgcatttctttatgccgggctttgccccgttaaccagtcgcggtagtcagcagtatcgcgcactgaccgttccggaactgacccagcaagtgttcgatgccaagaacatgatggcagcatgcgatccgcgtcatggccgctatctgaccgtggccgccgtttttcgtggtcgtatgagcatgaaggaagtggacgagcagatgctgaacgttcagaacaaaaacagcagctacttcgtggaatggatcccgaacaacgttaagaccgcagtgtgtgatatcccgccgcgcggtctgaaaatggccgtgacctttatcggcaacagcaccgccatccaggaattattcaaacgcattagcgaacagttcaccgccatgtttcgccgtaaagccttcctgcactggtataccggtgaaggcatggacgagatggagtttaccgaggccgagagtaacatgaatgacctggtgagcgagtaccagcagtatcaggatgccacagccgaggaagaagaagatttcggcgaagaagccgaagaggaagcataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z