BBa_K1891013
1
BBa_K1891013
beta-tubulin-C- Luciferase
2016-10-10T11:00:00Z
2016-10-11T08:03:32Z
Beta-tubulin comes from human breast cancer cell line Mcf-7 cDNA. C- Luciferase comes from pCambia1300-C-Luciferase plasmid.
It expresses beta-tubulin- C-Luciferase fusion protein with a 42bp linker. The C-terminal of beta-tubulin links to the N-terminal of C-Luciferase. We connected this two gene fragment using fusion PCR. And then we cloned it into pET30a(+) and add IPTG until its final concentration is 1mmol/L, incubate for 4-6h(37??C, 250rpm) to induce the expression of this fusion protein.
false
false
_2356_
27040
27040
9
false
We connected this two gene fragments using fusion PCR. First we acquired beta-tubulin gene fragment and C-Luciferase gene fragment. And then we redesigned a antisense primer of beta-Luciferase and a sense primer of C-Luciferase with a linker about 42bp. And then doing fusion PCR.
false
Zhao Jingyu
annotation2492385
1
C- Luciferase-beta-tubulin
range2492385
1
1
1919
BBa_K1891013_sequence
1
atgtccggttatgtaaacaatccggaagcgaccaacgccttgattgacaaggatggatggctacattctggagacatagcttactgggacgaagacgaacacttcttcatcgttgaccgcctgaagtctctgattaagtacaaaggctatcaggtggctcccgctgaattggaatccatcttgctccaacaccccaacatcttcgacgcaggtgtcgcaggtcttcccgacgatgacgccggtgaacttcccgccgccgttgttgttttggagcacggaaagacgatgacggaaaaagagatcgtggattacgtcgccagtcaagtaacaaccgcgaaaaagttgcgcggaggagttgtgtttgtggacgaagtaccgaaaggtcttaccggaaaactcgacgcaagaaaaatcagagagatcctcataaaggccaagaagggcggaaagatcgccgtgacaagtttgtacaaaaaagctgaacgagaaacgtaaaatgatataaatatcaatatattaaattagattttgcataaaaaacagactacataatactgtaaaacacaacatatccagtcactatgatgcgtgaaatcgtgcacattcaggcaggccagtgcggtaaccagatcggcgccaagttttgggaggttatcagcgacgagcacggcatcgatccgaccggtacctatcatggcgacagtgatttacaactggaccgtatcagcgtgtattacaacgaggccaccggcggtaaatacgtgccgcgtgccattctggtggatctggaaccgggtaccatggatagtgttcgtagcggcccgtttggtcagatctttcgcccggataactttgtgttcggccagagcggtgccggcaataactgggccaaaggccactatactgagggcgcagagctggtggatagcgtgctggacgtggtgcgcaaagaagccgaaagttgtgattgcttacaaggctttcagctgacccacagtctgggtggcggtaccggtagtggtatgggtaccctgctgatcagcaaaatccgcgaggaatacccggaccgcattatgaacaccttcagcgtggttccgagtccgaaagtgagcgacaccgtggttgaaccttacaatgccaccctgagcgtgcaccagctggttgagaataccgatgaaacctattgtatcgataatgaggccttatatgacatttgctttcgcacactgaagctgaccacccctacatatggcgatctgaaccatctggtgagcgcaaccatgagcggtgtgacaacctgtctgcgctttcctggccagctgaacgccgatttacgcaaactggccgtgaatatggttccgtttcctcgtctgcatttctttatgccgggctttgccccgttaaccagtcgcggtagtcagcagtatcgcgcactgaccgttccggaactgacccagcaagtgttcgatgccaagaacatgatggcagcatgcgatccgcgtcatggccgctatctgaccgtggccgccgtttttcgtggtcgtatgagcatgaaggaagtggacgagcagatgctgaacgttcagaacaaaaacagcagctacttcgtggaatggatcccgaacaacgttaagaccgcagtgtgtgatatcccgccgcgcggtctgaaaatggccgtgacctttatcggcaacagcaccgccatccaggaattattcaaacgcattagcgaacagttcaccgccatgtttcgccgtaaagccttcctgcactggtataccggtgaaggcatggacgagatggagtttaccgaggccgagagtaacatgaatgacctggtgagcgagtaccagcagtatcaggatgccacagccgaggaagaagaagatttcggcgaagaagccgaagaggaagcataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z