LOCUS       BBa_K1892004             831 bp    DNA     linear       01-Jan-2026
DEFINITION  J04500+yebf-LL-37+B0015
ACCESSION   BBa_K1892004
VERSION     BBa_K1892004.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    1..220
                     /label=BBa_J04500
     CDS             227..694
                     /label=BBa_K1892000
     stem_loop       714..757
                     /label=stem_loop
     misc_feature    703..782
                     /label=BBa_B0010
     terminator      703..782
                     /label=BBa_B0010
     stem_loop       798..817
                     /label=T7 TE
     misc_feature    824..824
                     /label=stop
     polyA_site      818..831
                     /label=polya
     misc_feature    791..831
                     /label=BBa_B0012
     terminator      791..831
                     /label=BBa_B0012
     terminator      703..831
                     /label=BBa_B0015
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgg gaaaaagagg
      241 ggcgttttta gggctgttgt tggtttctgc ctgcgcatca gttttcgctg ccaataatga
      301 aaccagcaag tcggtcactt tcccaaagtg tgaaggtctg gatgctgccg gaattgccgc
      361 gagcgtaaaa cgtgattatc aacaaaatcg cgtggcgcgc tgggcagatg atcaaaaaat
      421 tgtcggtcag gccgatcccg tggcttgggt cagtttgcag gacattcagg gtaaagatga
      481 taaatggtca gtaccgctaa ccgtgcgtgg taaaagtgcc gatattcatt accaggtcag
      541 cgtggactgc aaagcgggaa tggcggaata tcagcggcgt ctgctgggtg atttcttccg
      601 gaaatctaaa gagaagattg gcaaagagtt taaaagaatt gtccagagaa tcaaggattt
      661 tttgcggaat cttgtaccca ggacagagtc ctaatactag agccaggcat caaataaaac
      721 gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc
      781 tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat a
//