LOCUS       BBa_K1892005             990 bp    DNA     linear       01-Jun-2024
DEFINITION  J04500+SDF-ELP(20)+B0015
ACCESSION   BBa_K1892005
VERSION     BBa_K1892005.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    1..220
                     /label=BBa_J04500
     CDS             227..853
                     /label=BBa_K1892001
     stem_loop       873..916
                     /label=stem_loop
     misc_feature    862..941
                     /label=BBa_B0010
     terminator      862..941
                     /label=BBa_B0010
     stem_loop       957..976
                     /label=T7 TE
     misc_feature    983..983
                     /label=stop
     polyA_site      977..990
                     /label=polya
     misc_feature    950..990
                     /label=BBa_B0012
     terminator      950..990
                     /label=BBa_B0012
     terminator      862..990
                     /label=BBa_B0015
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatga atgcgaaagt
      241 cgttgtcgtg ctggtgttgg tcttaactgc actgtgtttg tctgatggta aaccggtgag
      301 tctttcgtac cgttgcccgt gccgtttctt cgaatcacat gttgctcgcg cgaacgtgaa
      361 acacctgaaa attttgaata cgccgaattg cgcactccag attgtggcgc gtctgaaaaa
      421 caataaccgc caggtatgca tcgaccctaa actgaagtgg attcaagaat atcttgaaaa
      481 agcacttaac aaagcgggcg cgggcgcggg cccggaaggc ggtggtggtg gtagcggtgg
      541 tggtggtagc gttccgggta aaggtgttcc gggtgttggt gttccgggtg ttggtgttcc
      601 gggtgttggt gttccgggtg ttggtgttcc gggtgttggt gttccgggtg ttggtgttcc
      661 gggtgttggt gttccgggtg ttggtgttcc gggttttggt gttccgggta aaggtgttcc
      721 gggtgttggt gttccgggtg ttggtgttcc gggtgttggt gttccgggtg ttggtgttcc
      781 gggtgttggt gttccgggtg ttggtgttcc gggtgttggt gttccgggtg ttggtgttcc
      841 gggttttggt taatactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact
      901 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct
      961 caccttcggg tgggcctttc tgcgtttata
//