BBa_R0010
1
LacI
promoter (lacI regulated)
2003-01-31T12:00:00Z
2015-05-08T01:14:14Z
The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
Released HQ 2013
Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
false
true
_1_
0
24
7
In stock
false
<P> <P><P> LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
true
annotation1961222
1
BBa_R0010
range1961222
1
1
200
annotation1961223
1
CAP binding site
range1961223
1
89
126
annotation1961225
1
-10
range1961225
1
161
166
annotation1961227
1
start
range1961227
1
173
173
annotation1961221
1
end of LacI coding region (inactive)
range1961221
1
1
88
annotation1961226
1
LacI binding site
range1961226
1
166
200
annotation1961224
1
-35
range1961224
1
137
142
BBa_J04500
1
BBa_J04500
IPTG inducible promoter with RBS
2005-06-08T11:00:00Z
2015-08-31T04:08:14Z
Davidson Synth-Aces
Released HQ 2013
R0010.B0034
false
true
_16_
0
326
16
In stock
false
false
Kristen DeCelle
component1508149
1
BBa_R0010
component1508159
1
BBa_B0034
annotation1508159
1
BBa_B0034
range1508159
1
209
220
annotation1508149
1
BBa_R0010
range1508149
1
1
200
BBa_B0015
1
BBa_B0015
double terminator (B0010-B0012)
2003-07-16T11:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Double terminator consisting of BBa_B0010 and BBa_B0012
false
true
_1_
0
24
7
In stock
false
true
Reshma Shetty
component1916612
1
BBa_B0012
component1916610
1
BBa_B0010
annotation1916610
1
BBa_B0010
range1916610
1
1
80
annotation1916612
1
BBa_B0012
range1916612
1
89
129
BBa_K1892001
1
BBa_K1892001
SDF-ELP(20)
2016-10-13T11:00:00Z
2016-10-19T02:47:17Z
Humans
a fusion protein which consists of the human growth factor SDF1 (Stromal cell-derived factor1 ) and an elastin- like peptide (ELP)
false
false
_2357_
24906
24906
9
false
The repeated times of pentapeptides
false
YingQi Wang
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation7018
1
BBa_B0010
range7018
1
1
80
annotation4184
1
stem_loop
range4184
1
12
55
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation7020
1
BBa_B0012
range7020
1
1
41
annotation1687
1
stop
range1687
1
34
34
annotation1690
1
polya
range1690
1
28
41
annotation1686
1
T7 TE
range1686
1
8
27
BBa_K1892005
1
BBa_K1892005
J04500+SDF-ELP(20)+B0015
2016-10-13T11:00:00Z
2016-10-20T03:05:10Z
No.
a fusion protein which consists of the human growth factor SDF1 (Stromal cell-derived factor1 ) and an elastin- like peptide (ELP) with an iPTG induced promoter, an RBS and a double terminator
false
false
_2357_
24906
24906
9
false
The repeated times of pentapeptides of elastin-like-protein
false
YingQi Wang
component2525103
1
BBa_J04500
component2525111
1
BBa_B0015
component2525104
1
BBa_K1892001
annotation2525104
1
BBa_K1892001
range2525104
1
227
853
annotation2525103
1
BBa_J04500
range2525103
1
1
220
annotation2525111
1
BBa_B0015
range2525111
1
862
990
BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_R0010_sequence
1
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca
BBa_B0034_sequence
1
aaagaggagaaa
BBa_K1892001_sequence
1
atgaatgcgaaagtcgttgtcgtgctggtgttggtcttaactgcactgtgtttgtctgatggtaaaccggtgagtctttcgtaccgttgcccgtgccgtttcttcgaatcacatgttgctcgcgcgaacgtgaaacacctgaaaattttgaatacgccgaattgcgcactccagattgtggcgcgtctgaaaaacaataaccgccaggtatgcatcgaccctaaactgaagtggattcaagaatatcttgaaaaagcacttaacaaagcgggcgcgggcgcgggcccggaaggcggtggtggtggtagcggtggtggtggtagcgttccgggtaaaggtgttccgggtgttggtgttccgggtgttggtgttccgggtgttggtgttccgggtgttggtgttccgggtgttggtgttccgggtgttggtgttccgggtgttggtgttccgggtgttggtgttccgggttttggtgttccgggtaaaggtgttccgggtgttggtgttccgggtgttggtgttccgggtgttggtgttccgggtgttggtgttccgggtgttggtgttccgggtgttggtgttccgggtgttggtgttccgggtgttggtgttccgggttttggttaa
BBa_J04500_sequence
1
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaa
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_B0015_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_K1892005_sequence
1
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgaatgcgaaagtcgttgtcgtgctggtgttggtcttaactgcactgtgtttgtctgatggtaaaccggtgagtctttcgtaccgttgcccgtgccgtttcttcgaatcacatgttgctcgcgcgaacgtgaaacacctgaaaattttgaatacgccgaattgcgcactccagattgtggcgcgtctgaaaaacaataaccgccaggtatgcatcgaccctaaactgaagtggattcaagaatatcttgaaaaagcacttaacaaagcgggcgcgggcgcgggcccggaaggcggtggtggtggtagcggtggtggtggtagcgttccgggtaaaggtgttccgggtgttggtgttccgggtgttggtgttccgggtgttggtgttccgggtgttggtgttccgggtgttggtgttccgggtgttggtgttccgggtgttggtgttccgggtgttggtgttccgggttttggtgttccgggtaaaggtgttccgggtgttggtgttccgggtgttggtgttccgggtgttggtgttccgggtgttggtgttccgggtgttggtgttccgggtgttggtgttccgggtgttggtgttccgggtgttggtgttccgggttttggttaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z