LOCUS       BBa_K1893007            1911 bp    DNA     linear       17-May-2024
DEFINITION  Cin receiver with GFP (CinR+pCin+GFP)
ACCESSION   BBa_K1893007
VERSION     BBa_K1893007.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23101
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     CDS             64..809
                     /label=CinR
     CDS             64..809
                     /label=BBa_K1893028
     stem_loop       829..872
                     /label=stem_loop
     misc_feature    818..897
                     /label=BBa_B0010
     terminator      818..897
                     /label=BBa_B0010
     stem_loop       913..932
                     /label=T7 TE
     misc_feature    939..939
                     /label=stop
     polyA_site      933..946
                     /label=polya
     misc_feature    906..946
                     /label=BBa_B0012
     terminator      906..946
                     /label=BBa_B0012
     terminator      818..946
                     /label=BBa_B0015
     promoter        955..1028
                     /label=pCin
     promoter        955..1028
                     /label=BBa_K1893029
     misc_feature    1..1028
                     /label=BBa_K1893006
     misc_feature    1041..1044
                     /label=conserved
     RBS             1037..1048
                     /label=BBa_B0034
     CDS             1055..1774
                     /label=GFP protein
     CDS             1055..1774
                     /label=BBa_E0040
     stem_loop       1794..1837
                     /label=stem_loop
     misc_feature    1783..1862
                     /label=BBa_B0010
     terminator      1783..1862
                     /label=BBa_B0010
     stem_loop       1878..1897
                     /label=T7 TE
     misc_feature    1904..1904
                     /label=stop
     polyA_site      1898..1911
                     /label=polya
     misc_feature    1871..1911
                     /label=BBa_B0012
     terminator      1871..1911
                     /label=BBa_B0012
     misc_feature    1037..1911
                     /label=BBa_I13504
ORIGIN
        1 tttacagcta gctcagtcct aggtattatg ctagctacta gagaaagagg agaaatacta
       61 gagtgctaag gagaaattct agtatgattg agaataccta tagcgaaaag ttcgagtccg
      121 cgttcgaaca gatcaaggcg gcggccaacg tggatgccgc catccgtatt ctccaggcgg
      181 aatataacct cgatttcgtc acctaccatc tcgcccagac gatcgcgagc aagatcgatc
      241 cgcccttcgt gcgcaccacc tatccggatg cctgggtttc ccgctacctc ctcaacagct
      301 atgtgaaggt cgatccgatc gtcaagcagg gcttcgaacg ccagctgccc ttcgactgga
      361 gcgaggtcga accgacgccg gaggcctatg ccatgctggt cgacgcccag aaacacggca
      421 tcggtggcaa tggctactcc atccccgtcg ccgacaaggc gcagcgccgc gccctgctgt
      481 cgctgaatgc ccgtataccg gccgacgaat ggaccgagct tgtgcgccgc tgccgcaacg
      541 agtggatcga gatcgcccat ctgatccacc gcaaggccgt ctatgagctg catggcgaaa
      601 acgatccggt gccggcattg tcgccgcgcg agatcgagtg tctgcactgg accgccctcg
      661 gcaaggatta caaggatatt tcggtcatcc tgggcatatc agagcatacc acacgcgatt
      721 acctgaagac cgcccgcttc aagctcggct gcgccacgat ctcggccgcc gcgtcgcggg
      781 ctgttcaatt gcgcatcatc aatccctgat actagagcca ggcatcaaat aaaacgaaag
      841 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac
      901 tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttatatact agagggggcc
      961 tatctgaggg aatttacttc cctatcagtg atagagatac tgagcacatc cctatcagtg
     1021 atagagatta ctagagaaag aggagaaata ctagatgcgt aaaggagaag aacttttcac
     1081 tggagttgtc ccaattcttg ttgaattaga tggtgatgtt aatgggcaca aattttctgt
     1141 cagtggagag ggtgaaggtg atgcaacata cggaaaactt acccttaaat ttatttgcac
     1201 tactggaaaa ctacctgttc catggccaac acttgtcact actttcggtt atggtgttca
     1261 atgctttgcg agatacccag atcatatgaa acagcatgac tttttcaaga gtgccatgcc
     1321 cgaaggttat gtacaggaaa gaactatatt tttcaaagat gacgggaact acaagacacg
     1381 tgctgaagtc aagtttgaag gtgataccct tgttaataga atcgagttaa aaggtattga
     1441 ttttaaagaa gatggaaaca ttcttggaca caaattggaa tacaactata actcacacaa
     1501 tgtatacatc atggcagaca aacaaaagaa tggaatcaaa gttaacttca aaattagaca
     1561 caacattgaa gatggaagcg ttcaactagc agaccattat caacaaaata ctccaattgg
     1621 cgatggccct gtccttttac cagacaacca ttacctgtcc acacaatctg ccctttcgaa
     1681 agatcccaac gaaaagagag accacatggt ccttcttgag tttgtaacag ctgctgggat
     1741 tacacatggc atggatgaac tatacaaata ataatactag agccaggcat caaataaaac
     1801 gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc
     1861 tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat a
//