BBa_K1893033 1 RBS Strong ribosome binding site (RBS) 2016-10-22T11:00:00Z 2016-10-27T02:35:55Z Same sequence that was used by James Chappell (Nature Chemical Biology, 2015) in designing the STAR reporter plasmid. Strong RBS false false _2358_ 31914 31815 9 false Was synthesized as a block with j23119, STAR-target, SFGFP, TrrnB false Akash Bhattacharjee BBa_K1893032 1 SF-GFP Superfolder GFP (SF-GFP) 2016-10-21T11:00:00Z 2016-10-27T02:35:28Z This sequence is taken from the construct as used by James Chapelle, for the STAR reporter plasmid. Reporter protein superfolder GFP (SFGFP) false false _2358_ 31914 31815 9 false This was synthesised as a block with j23119, STAR target. false Akash Bhattacharjee annotation2528178 1 SFGFP range2528178 1 1 717 BBa_K1893009 1 STAR targe STAR inducible superfolder GFP (pAD1.S5+SF-GFP) 2016-10-13T11:00:00Z 2016-10-28T07:57:17Z More Stuff Stuff false false _2358_ 31815 31914 9 false Lots of stuff false Akash Bhattacharjee, Gear Rotrattanadumrong component2528177 1 BBa_K1893035 component2528173 1 BBa_K1893031 component2528172 1 BBa_J23119 component2528176 1 BBa_K112999 component2528175 1 BBa_K1893032 component2528174 1 BBa_K1893033 annotation2528177 1 BBa_K1893035 range2528177 1 872 1239 annotation2528172 1 BBa_J23119 range2528172 1 1 35 annotation2528173 1 BBa_K1893031 range2528173 1 36 128 annotation2528174 1 BBa_K1893033 range2528174 1 129 148 annotation2528175 1 BBa_K1893032 range2528175 1 149 865 annotation2528176 1 BBa_K112999 range2528176 1 866 871 BBa_K1893035 1 TrrnB rrnB terminator (TrrnB) 2016-10-22T11:00:00Z 2016-10-27T02:36:20Z Comes from James Chappell's paper (Nature Chemical Biology, 2015), from the STAR reporter construct. Transcription terminator (rrnB terminator) false false _2358_ 31914 31815 9 false Was syntheszed as a block along with j23119, STAR-target, SFGFP. false Akash Bhattacharjee BBa_K112999 1 scar BBb1 scar sequence 2008-10-20T11:00:00Z 2015-05-08T01:09:20Z This is the scar sequence of the BBb1 assembly scheme. For more information, refer to [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]. false false _224_ 0 2998 9 Not in stock false false Bing Xia BBa_J23119 1 BBa_J23119 constitutive promoter family member 2006-08-23T11:00:00Z 2015-08-31T04:08:40Z Overlap extension of synthetic oligonucleotides Released HQ 2013 Later false true _52_ 0 483 95 In stock false N/A true John Anderson BBa_K1893031 1 STAR targe STAR target (pAD1.S5) 2016-10-21T11:00:00Z 2016-10-27T02:34:01Z This comes from the pAD1 attenuator sequence. When transcribed, it produces the sense RNA which is also complementary to the STAR-antisense RNA. In the absence of STAR, it forms a hairpin structure and and prevent transcription of the downstream coding sequence. false false _2358_ 31914 31815 9 false This was synthesised as a composite sequence including the j23119 promoter, SFGFP and TrrnB terminator. false Akash Bhattacharjee BBa_K1893009_sequence 1 ttgacagctagctcagtcctaggtataatgctagcagtttttacagtgaattgttttaattagttgtataaatgttggagcagcggggaatgtatacagttcatgtatatattccccgctttttttttggatctaggaggaaggatctatgagcaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtccgtggagagggtgaaggtgatgctacaaacggaaaactcacccttaaatttatttgcactactggaaaactacctgttccgtggccaacacttgtcactactctgacctatggtgttcaatgcttttcccgttatccggatcacatgaaacggcatgactttttcaagagtgccatgcccgaaggttatgtacaggaacgcactatatctttcaaagatgacgggacctacaagacgcgtgctgaagtcaagtttgaaggtgatacccttgttaatcgtatcgagttaaagggtattgattttaaagaagatggaaacattcttggacacaaactcgagtacaactttaactcacacaatgtatacatcacggcagacaaacaaaagaatggaatcaaagctaacttcaaaattcgccacaacgttgaagatggttccgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtcgacacaatctgtcctttcgaaagatcccaacgaaaagcgtgaccacatggtccttcttgagtttgtaactgctgctgggattacacatggcatggatgagctctacaaataaggatctgaagcttgggcccgaacaaaaactcatctcagaagaggatctgaatagcgccgtcgaccatcatcatcatcatcattgagtttaaacggtctccagcttggctgttttggcggatgagagaagattttcagcctgatacagattaaatcagaacgcagaagcggtctgataaaacagaatttgcctggcggcagtagcgcggtggtcccacctgaccccatgccgaactcagaagtgaaacgccgtagcgccgatggtagtgtggggtctccccatgcgagagtagggaactgccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaact BBa_K1893035_sequence 1 gaagcttgggcccgaacaaaaactcatctcagaagaggatctgaatagcgccgtcgaccatcatcatcatcatcattgagtttaaacggtctccagcttggctgttttggcggatgagagaagattttcagcctgatacagattaaatcagaacgcagaagcggtctgataaaacagaatttgcctggcggcagtagcgcggtggtcccacctgaccccatgccgaactcagaagtgaaacgccgtagcgccgatggtagtgtggggtctccccatgcgagagtagggaactgccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaact BBa_K1893032_sequence 1 atgagcaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtccgtggagagggtgaaggtgatgctacaaacggaaaactcacccttaaatttatttgcactactggaaaactacctgttccgtggccaacacttgtcactactctgacctatggtgttcaatgcttttcccgttatccggatcacatgaaacggcatgactttttcaagagtgccatgcccgaaggttatgtacaggaacgcactatatctttcaaagatgacgggacctacaagacgcgtgctgaagtcaagtttgaaggtgatacccttgttaatcgtatcgagttaaagggtattgattttaaagaagatggaaacattcttggacacaaactcgagtacaactttaactcacacaatgtatacatcacggcagacaaacaaaagaatggaatcaaagctaacttcaaaattcgccacaacgttgaagatggttccgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtcgacacaatctgtcctttcgaaagatcccaacgaaaagcgtgaccacatggtccttcttgagtttgtaactgctgctgggattacacatggcatggatgagctctacaaataa BBa_K1893031_sequence 1 agtttttacagtgaattgttttaattagttgtataaatgttggagcagcggggaatgtatacagttcatgtatatattccccgcttttttttt BBa_K112999_sequence 1 ggatct BBa_K1893033_sequence 1 ggatctaggaggaaggatct BBa_J23119_sequence 1 ttgacagctagctcagtcctaggtataatgctagc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z