LOCUS       BBa_K1893017            2230 bp    DNA     linear       26-Nov-2024
DEFINITION  Arabinose inducible GFP (pBAD+GFP)
ACCESSION   BBa_K1893017
VERSION     BBa_K1893017.1  
FEATURES             Location/Qualifiers
     misc_feature    1..41
                     /label=B0012
     misc_feature    50..129
                     /label=B0010
     terminator      1..129
                     /label=BBa_B0025
     CDS             138..1178
                     /label=AraC
     promoter        1179..1252
                     /label=PC
     promoter        1253..1347
                     /label=pBAD
     promoter        138..1347
                     /label=BBa_K1321333
     promoter        1..1347
                     /label=BBa_K1893015
     misc_feature    1360..1363
                     /label=conserved
     RBS             1356..1367
                     /label=BBa_B0034
     CDS             1374..2093
                     /label=GFP protein
     CDS             1374..2093
                     /label=BBa_E0040
     stem_loop       2113..2156
                     /label=stem_loop
     misc_feature    2102..2181
                     /label=BBa_B0010
     terminator      2102..2181
                     /label=BBa_B0010
     stem_loop       2197..2216
                     /label=T7 TE
     misc_feature    2223..2223
                     /label=stop
     polyA_site      2217..2230
                     /label=polya
     misc_feature    2190..2230
                     /label=BBa_B0012
     terminator      2190..2230
                     /label=BBa_B0012
     misc_feature    1356..2230
                     /label=BBa_I13504
ORIGIN
        1 tataaacgca gaaaggccca cccgaaggtg agccagtgtg actctagtag agagcgttca
       61 ccgacaaaca acagataaaa cgaaaggccc agtctttcga ctgagccttt cgttttattt
      121 gatgcctggt actagagtta tgacaacttg acggctacat cattcacttt ttcttcacaa
      181 ccggcacgga actcgctcgg gctggccccg gtgcattttt taaatacccg cgagaaatag
      241 agttgatcgt caaaaccaac attgcgaccg acggtggcga taggcatccg ggtggtgctc
      301 aaaagcagct tcgcctggct gatacgttgg tcctcgcgcc agcttaagac gctaatccct
      361 aactgctggc ggaaaagatg tgacagacgc gacggcgaca agcaaacatg ctgtgcgacg
      421 ctggcgatat caaaattgct gtctgccagg tgatcgctga tgtactgaca agcctcgcgt
      481 acccgattat ccatcggtgg atggagcgac tcgttaatcg cttccatgcg ccgcagtaac
      541 aattgctcaa gcagatttat cgccagcagc tccgaatagc gcccttcccc ttgcccggcg
      601 ttaatgattt gcccaaacag gtcgctgaaa tgcggctggt gcgcttcatc cgggcgaaag
      661 aaccccgtat tggcaaatat tgacggccag ttaagccatt catgccagta ggcgcgcgga
      721 cgaaagtaaa cccactggtg ataccattcg cgagcctccg gatgacgacc gtagtgatga
      781 atctctcctg gcgggaacag caaaatatca cccggtcggc aaacaaattc tcgtccctga
      841 tttttcacca ccccctgacc gcgaatggtg agattgagaa tataaccttt cattcccagc
      901 ggtcggtcga taaaaaaatc gagataaccg ttggcctcaa tcggcgttaa acccgccacc
      961 agatgggcat taaacgagta tcccggcagc aggggatcat tttgcgcttc agccatactt
     1021 ttcatactcc cgccattcag agaagaaacc aattgtccat attgcatcag acattgccgt
     1081 cactgcgtct tttactggct cttctcgcta accaaaccgg taaccccgct tattaaaagc
     1141 attctgtaac aaagcgggac caaagccatg acaaaaacgc gtaacaaaag tgtctataat
     1201 cacggcagaa aagtccacat tgattatttg cacggcgtca cactttgcta tgccatagca
     1261 tttttatcca taagattagc ggatcctacc tgacgctttt tatcgcaact ctctactgtt
     1321 tctccatacc cgtttttttg ggctagctac tagagaaaga ggagaaatac tagatgcgta
     1381 aaggagaaga acttttcact ggagttgtcc caattcttgt tgaattagat ggtgatgtta
     1441 atgggcacaa attttctgtc agtggagagg gtgaaggtga tgcaacatac ggaaaactta
     1501 cccttaaatt tatttgcact actggaaaac tacctgttcc atggccaaca cttgtcacta
     1561 ctttcggtta tggtgttcaa tgctttgcga gatacccaga tcatatgaaa cagcatgact
     1621 ttttcaagag tgccatgccc gaaggttatg tacaggaaag aactatattt ttcaaagatg
     1681 acgggaacta caagacacgt gctgaagtca agtttgaagg tgataccctt gttaatagaa
     1741 tcgagttaaa aggtattgat tttaaagaag atggaaacat tcttggacac aaattggaat
     1801 acaactataa ctcacacaat gtatacatca tggcagacaa acaaaagaat ggaatcaaag
     1861 ttaacttcaa aattagacac aacattgaag atggaagcgt tcaactagca gaccattatc
     1921 aacaaaatac tccaattggc gatggccctg tccttttacc agacaaccat tacctgtcca
     1981 cacaatctgc cctttcgaaa gatcccaacg aaaagagaga ccacatggtc cttcttgagt
     2041 ttgtaacagc tgctgggatt acacatggca tggatgaact atacaaataa taatactaga
     2101 gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt
     2161 tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc
     2221 tgcgtttata
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