LOCUS       BBa_K1895004            1297 bp    DNA     linear       22-Nov-2024
DEFINITION  Porin Overexpression for Microbial Fuel Cell Use
ACCESSION   BBa_K1895004
VERSION     BBa_K1895004.1  
FEATURES             Location/Qualifiers
     promoter        1..131
                     /label=promoter
     misc_feature    40..57
                     /label=AraI1
     misc_feature    61..78
                     /label=AraI2
     promoter        1..130
                     /label=BBa_K206000
     misc_feature    143..146
                     /label=conserved
     RBS             139..150
                     /label=BBa_B0034
     CDS             157..1248
                     /label=ompF
     misc_feature    222..222
                     /label=T>A
     misc_feature    225..225
                     /label=A>T
     CDS             157..1248
                     /label=BBa_K864204
     stem_loop       1264..1283
                     /label=T7 TE
     misc_feature    1290..1290
                     /label=stop
     polyA_site      1284..1297
                     /label=polya
     misc_feature    1257..1297
                     /label=BBa_B0012
     terminator      1257..1297
                     /label=BBa_B0012
ORIGIN
        1 acattgatta tttgcacggc gtcacacttt gctatgccat agcaagatag tccataagat
       61 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca taccgttttt
      121 ttgggctagc tactagagaa agaggagaaa tactagatga tgaagcgcaa tattctggca
      181 gtgatcgtcc ctgctctgtt agtagcaggt actgcaaacg cagctgaaat ctataacaaa
      241 gatggcaaca aagtagatct gtacggtaaa gctgttggtc tgcattattt ttccaagggt
      301 aacggtgaaa acagttacgg tggcaatggc gacatgacct atgcccgtct tggttttaaa
      361 ggggaaactc aaatcaattc cgatctgacc ggttatggtc agtgggaata taacttccag
      421 ggtaacaact ctgaaggcgc tgacgctcaa actggtaaca aaacgcgtct ggcattcgcg
      481 ggtcttaaat acgctgacgt tggttctttc gattacggcc gtaactacgg tgtggtttat
      541 gatgcactgg gttacaccga tatgctgcca gaatttggtg gtgatactgc atacagcgat
      601 gacttcttcg ttggtcgtgt tggcggcgtt gctacctatc gtaactccaa cttctttggt
      661 ctggttgatg gcctgaactt cgctgttcag tacctgggta aaaacgagcg tgacactgca
      721 cgccgttcta acggcgacgg tgttggcggt tctatcagct acgaatacga aggctttggt
      781 atcgttggtg cttatggtgc agctgaccgt accaacctgc aagaagctca acctcttggc
      841 aacggtaaaa aagctgaaca gtgggctact ggtctgaagt acgacgcgaa caacatctac
      901 ctggcagcga actacggtga aacccgtaac gctacgccga tcactaataa atttacaaac
      961 accagcggct tcgccaacaa aacgcaagac gttctgttag ttgcgcaata ccagttcgat
     1021 ttcggtctgc gtccgtccat cgcttacacc aaatctaaag cgaaagacgt agaaggtatc
     1081 ggtgatgttg atctggtgaa ctactttgaa gtgggcgcaa cctactactt caacaaaaac
     1141 atgtccacct atgttgacta catcatcaac cagatcgatt ctgacaacaa actgggcgta
     1201 ggttcagacg acaccgttgc tgtgggtatc gtttaccagt tctaataata ctagagtcac
     1261 actggctcac cttcgggtgg gcctttctgc gtttata
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