BBa_K1895995
type: ComponentDefinition
persistent identity: BBa K1895995
display id: BBa_K1895995
version: 1
was generated by: iGEM to SBOL conversion
description: Time delay device
created: 2016-07-21T11:00:00Z
modified: 2016-07-22T07:03:08Z
mutable provenance: Hypothetical part from the 2016 Newcastle iGEM Project.
owned by: james
owned by: myers
mutable description: [TODO]
discontinued: false
dominant: false
group u list: _2361_
m user id: 29859
owner id: 29859
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: [TODO]
star: false
creator: Jake Burton
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_R0010
component: BBa_C0171
component: BBa_B0034
component: BBa_C0052
component: BBa_I14017
component: BBa_B1006
component: BBa_C0051
component: BBa_I12006
component: BBa_I746916
sequence annotation: BBa_R0010
sequence annotation: BBa_B0034
sequence annotation: BBa_C0171
sequence annotation: BBa_C0052
sequence annotation: BBa_B1006
sequence annotation: BBa_I14017
sequence annotation: BBa_C0051
sequence annotation: BBa_I12006
sequence annotation: BBa_I746916
sequence: https://synbiohub.org/public/igem/BBa_K1895995_sequence/1

BBa_R0010
type: Component
persistent identity: component2481250
display id: component2481250
version: 1
top level: BBa_K1895995
definition: LacI
access: public

BBa_C0171
type: Component
persistent identity: component2481259
display id: component2481259
version: 1
top level: BBa_K1895995
definition: rhIR
access: public

BBa_B0034
type: Component
persistent identity: component2481258
display id: component2481258
version: 1
top level: BBa_K1895995
definition: BBa_B0034
access: public

BBa_C0052
type: Component
persistent identity: component2481263
display id: component2481263
version: 1
top level: BBa_K1895995
definition: cI 434
access: public

BBa_B0034
type: Component
persistent identity: component2481261
display id: component2481261
version: 1
top level: BBa_K1895995
definition: BBa_B0034
access: public

BBa_C0052
type: Component
persistent identity: component2481269
display id: component2481269
version: 1
top level: BBa_K1895995
definition: cI 434
access: public

BBa_B0034
type: Component
persistent identity: component2481267
display id: component2481267
version: 1
top level: BBa_K1895995
definition: BBa_B0034
access: public

BBa_I14017
type: Component
persistent identity: component2481280
display id: component2481280
version: 1
top level: BBa_K1895995
definition: RhI
access: public

BBa_B1006
type: Component
persistent identity: component2481276
display id: component2481276
version: 1
top level: BBa_K1895995
definition: BBa_B1006
access: public

BBa_C0051
type: Component
persistent identity: component2481286
display id: component2481286
version: 1
top level: BBa_K1895995
definition: cI lam
access: public

BBa_B0034
type: Component
persistent identity: component2481282
display id: component2481282
version: 1
top level: BBa_K1895995
definition: BBa_B0034
access: public

BBa_I12006
type: Component
persistent identity: component2481297
display id: component2481297
version: 1
top level: BBa_K1895995
definition: Prm +
access: public

BBa_B1006
type: Component
persistent identity: component2481291
display id: component2481291
version: 1
top level: BBa_K1895995
definition: BBa_B1006
access: public

BBa_I746916
type: Component
persistent identity: component2481303
display id: component2481303
version: 1
top level: BBa_K1895995
definition: BBa_I746916
access: public

BBa_B0034
type: Component
persistent identity: component2481299
display id: component2481299
version: 1
top level: BBa_K1895995
definition: BBa_B0034
access: public

BBa_B1006
type: Component
persistent identity: component2481308
display id: component2481308
version: 1
top level: BBa_K1895995
definition: BBa_B1006
access: public

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2481250
display id: annotation2481250
version: 1
top level: BBa_K1895995
location: https://synbiohub.org/public/igem/BBa_K1895995/annotation2481250/range2481250/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K1895995/annotation2481250/range2481250/1
type: Range
persistent identity: range2481250
display id: range2481250
version: 1
top level: BBa_K1895995
direction: direction/forward
start: 1
end: 200
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2481258
display id: annotation2481258
version: 1
top level: BBa_K1895995
location: https://synbiohub.org/public/igem/BBa_K1895995/annotation2481258/range2481258/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1895995/annotation2481258/range2481258/1
type: Range
persistent identity: range2481258
display id: range2481258
version: 1
top level: BBa_K1895995
direction: direction/reverse
start: 209
end: 220
orientation: inline

BBa_C0171
type: SequenceAnnotation
persistent identity: annotation2481259
display id: annotation2481259
version: 1
top level: BBa_K1895995
location: https://synbiohub.org/public/igem/BBa_K1895995/annotation2481259/range2481259/1
component: BBa_C0171

https://synbiohub.org/public/igem/BBa_K1895995/annotation2481259/range2481259/1
type: Range
persistent identity: range2481259
display id: range2481259
version: 1
top level: BBa_K1895995
direction: direction/reverse
start: 227
end: 955
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2481261
display id: annotation2481261
version: 1
top level: BBa_K1895995
location: https://synbiohub.org/public/igem/BBa_K1895995/annotation2481261/range2481261/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1895995/annotation2481261/range2481261/1
type: Range
persistent identity: range2481261
display id: range2481261
version: 1
top level: BBa_K1895995
direction: direction/reverse
start: 964
end: 975
orientation: inline

BBa_C0052
type: SequenceAnnotation
persistent identity: annotation2481263
display id: annotation2481263
version: 1
top level: BBa_K1895995
location: https://synbiohub.org/public/igem/BBa_K1895995/annotation2481263/range2481263/1
component: BBa_C0052

https://synbiohub.org/public/igem/BBa_K1895995/annotation2481263/range2481263/1
type: Range
persistent identity: range2481263
display id: range2481263
version: 1
top level: BBa_K1895995
direction: direction/forward
start: 982
end: 1650
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2481267
display id: annotation2481267
version: 1
top level: BBa_K1895995
location: https://synbiohub.org/public/igem/BBa_K1895995/annotation2481267/range2481267/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1895995/annotation2481267/range2481267/1
type: Range
persistent identity: range2481267
display id: range2481267
version: 1
top level: BBa_K1895995
direction: direction/reverse
start: 1684
end: 1695
orientation: inline

BBa_C0052
type: SequenceAnnotation
persistent identity: annotation2481269
display id: annotation2481269
version: 1
top level: BBa_K1895995
location: https://synbiohub.org/public/igem/BBa_K1895995/annotation2481269/range2481269/1
component: BBa_C0052

https://synbiohub.org/public/igem/BBa_K1895995/annotation2481269/range2481269/1
type: Range
persistent identity: range2481269
display id: range2481269
version: 1
top level: BBa_K1895995
direction: direction/forward
start: 1702
end: 2370
orientation: inline

BBa_B1006
type: SequenceAnnotation
persistent identity: annotation2481276
display id: annotation2481276
version: 1
top level: BBa_K1895995
location: https://synbiohub.org/public/igem/BBa_K1895995/annotation2481276/range2481276/1
component: BBa_B1006

https://synbiohub.org/public/igem/BBa_K1895995/annotation2481276/range2481276/1
type: Range
persistent identity: range2481276
display id: range2481276
version: 1
top level: BBa_K1895995
direction: direction/reverse
start: 2404
end: 2442
orientation: inline

BBa_I14017
type: SequenceAnnotation
persistent identity: annotation2481280
display id: annotation2481280
version: 1
top level: BBa_K1895995
location: https://synbiohub.org/public/igem/BBa_K1895995/annotation2481280/range2481280/1
component: BBa_I14017

https://synbiohub.org/public/igem/BBa_K1895995/annotation2481280/range2481280/1
type: Range
persistent identity: range2481280
display id: range2481280
version: 1
top level: BBa_K1895995
direction: direction/reverse
start: 2451
end: 2501
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2481282
display id: annotation2481282
version: 1
top level: BBa_K1895995
location: https://synbiohub.org/public/igem/BBa_K1895995/annotation2481282/range2481282/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1895995/annotation2481282/range2481282/1
type: Range
persistent identity: range2481282
display id: range2481282
version: 1
top level: BBa_K1895995
direction: direction/reverse
start: 2510
end: 2521
orientation: inline

BBa_C0051
type: SequenceAnnotation
persistent identity: annotation2481286
display id: annotation2481286
version: 1
top level: BBa_K1895995
location: https://synbiohub.org/public/igem/BBa_K1895995/annotation2481286/range2481286/1
component: BBa_C0051

https://synbiohub.org/public/igem/BBa_K1895995/annotation2481286/range2481286/1
type: Range
persistent identity: range2481286
display id: range2481286
version: 1
top level: BBa_K1895995
direction: direction/reverse
start: 2528
end: 3302
orientation: inline

BBa_B1006
type: SequenceAnnotation
persistent identity: annotation2481291
display id: annotation2481291
version: 1
top level: BBa_K1895995
location: https://synbiohub.org/public/igem/BBa_K1895995/annotation2481291/range2481291/1
component: BBa_B1006

https://synbiohub.org/public/igem/BBa_K1895995/annotation2481291/range2481291/1
type: Range
persistent identity: range2481291
display id: range2481291
version: 1
top level: BBa_K1895995
direction: direction/reverse
start: 3311
end: 3349
orientation: inline

BBa_I12006
type: SequenceAnnotation
persistent identity: annotation2481297
display id: annotation2481297
version: 1
top level: BBa_K1895995
location: https://synbiohub.org/public/igem/BBa_K1895995/annotation2481297/range2481297/1
component: BBa_I12006

https://synbiohub.org/public/igem/BBa_K1895995/annotation2481297/range2481297/1
type: Range
persistent identity: range2481297
display id: range2481297
version: 1
top level: BBa_K1895995
direction: direction/reverse
start: 3358
end: 3439
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2481299
display id: annotation2481299
version: 1
top level: BBa_K1895995
location: https://synbiohub.org/public/igem/BBa_K1895995/annotation2481299/range2481299/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1895995/annotation2481299/range2481299/1
type: Range
persistent identity: range2481299
display id: range2481299
version: 1
top level: BBa_K1895995
direction: direction/reverse
start: 3448
end: 3459
orientation: inline

BBa_I746916
type: SequenceAnnotation
persistent identity: annotation2481303
display id: annotation2481303
version: 1
top level: BBa_K1895995
location: https://synbiohub.org/public/igem/BBa_K1895995/annotation2481303/range2481303/1
component: BBa_I746916

https://synbiohub.org/public/igem/BBa_K1895995/annotation2481303/range2481303/1
type: Range
persistent identity: range2481303
display id: range2481303
version: 1
top level: BBa_K1895995
direction: direction/reverse
start: 3466
end: 4185
orientation: inline

BBa_B1006
type: SequenceAnnotation
persistent identity: annotation2481308
display id: annotation2481308
version: 1
top level: BBa_K1895995
location: https://synbiohub.org/public/igem/BBa_K1895995/annotation2481308/range2481308/1
component: BBa_B1006

https://synbiohub.org/public/igem/BBa_K1895995/annotation2481308/range2481308/1
type: Range
persistent identity: range2481308
display id: range2481308
version: 1
top level: BBa_K1895995
direction: direction/reverse
start: 4194
end: 4232
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

LacI
type: ComponentDefinition
persistent identity: BBa R0010
display id: BBa_R0010
version: 1
was generated by: iGEM to SBOL conversion
description: promoter (lacI regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
star: true
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: end of LacI coding region (inactive)
sequence annotation: BBa_R0010
sequence annotation: CAP binding site
sequence annotation: -35
sequence annotation: -10
sequence annotation: LacI binding site
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_R0010_sequence/1

end of LacI coding region (inactive)
type: SequenceAnnotation
persistent identity: annotation1961221
display id: annotation1961221
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
type: Range
persistent identity: range1961221
display id: range1961221
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 88
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1961222
display id: annotation1961222
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
type: Range
persistent identity: range1961222
display id: range1961222
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 200
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation1961223
display id: annotation1961223
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
type: Range
persistent identity: range1961223
display id: range1961223
version: 1
top level: LacI
direction: direction/forward
start: 89
end: 126
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1961224
display id: annotation1961224
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
type: Range
persistent identity: range1961224
display id: range1961224
version: 1
top level: LacI
direction: direction/forward
start: 137
end: 142
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1961225
display id: annotation1961225
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
type: Range
persistent identity: range1961225
display id: range1961225
version: 1
top level: LacI
direction: direction/forward
start: 161
end: 166
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation1961226
display id: annotation1961226
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
type: Range
persistent identity: range1961226
display id: range1961226
version: 1
top level: LacI
direction: direction/forward
start: 166
end: 200
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1961227
display id: annotation1961227
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
type: Range
persistent identity: range1961227
display id: range1961227
version: 1
top level: LacI
direction: direction/forward
start: 173
end: 173
orientation: inline

rhIR
type: ComponentDefinition
persistent identity: BBa C0171
display id: BBa_C0171
version: 1
was generated by: iGEM to SBOL conversion
description: rhlR repressor/activator from P. aeruginosa PA3477 (no LVA)
created: 2004-05-26T11:00:00Z
modified: 2015-08-31T04:07:24Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: same as C0071 except no LVA tag
discontinued: false
dominant: false
experience: experience/None
group u list: _11_1_
m user id: 0
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jcbraff
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_C0171_sequence/1

cI lam
type: ComponentDefinition
persistent identity: BBa C0051
display id: BBa_C0051
version: 1
was generated by: iGEM to SBOL conversion
description: cI repressor from E. coli phage lambda (+LVA)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999).
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Coding region for the cI repressor based on cI repressor from bacteriophage lambda modified with an LVA tail for rapid degradation of the protein. cI repressor binds to the cI regulator (BBa_R0051).
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: References (unparsed) here: A synthetic oscillatory network of transcriptional regulators , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000 Octamerization of CI repressor is needed for effective repression of PRM and efficient switching from lysogeny. Ian B. Dodd,1 Alison J. Perkins, Daniel Tsemitsidis, and J. Barry Egan , Genes and Development (Vol 15, No. 22) 3013-3022: 2001 References (unparsed) here: A synthetic oscillatory network of transcriptional regulators , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000 Octamerization of CI repressor is needed for effective repression of PRM and efficient switching from lysogeny. Ian B. Dodd,1 Alison J. Perkins, Daniel Tsemitsidis, and J. Barry Egan , Genes and Development (Vol 15, No. 22) 3013-3022: 2001 BBa_C0051 cI repressor is based on the cI repressor from the Elowitz's repressilator. It has been modified to include a rapid degradation LAA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.
star: true
creator: Vinay S Mahajan, Brian Chow, Peter Carr, Voichita Marinescu and Alexander D. Wissner-Gross
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: cI lambda
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0051_sequence/1

cI lambda
type: SequenceAnnotation
persistent identity: annotation23334
display id: annotation23334
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_C0051/annotation23334/range23334/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_C0051/annotation23334/range23334/1
type: Range
persistent identity: range23334
display id: range23334
version: 1
top level: cI lam
direction: direction/forward
start: 4
end: 711
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation23335
display id: annotation23335
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_C0051/annotation23335/range23335/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_C0051/annotation23335/range23335/1
type: Range
persistent identity: range23335
display id: range23335
version: 1
top level: cI lam
direction: direction/forward
start: 712
end: 744
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213991
display id: annotation2213991
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_C0051/annotation2213991/range2213991/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_C0051/annotation2213991/range2213991/1
type: Range
persistent identity: range2213991
display id: range2213991
version: 1
top level: cI lam
direction: direction/forward
start: 751
end: 775
orientation: inline

cI 434
type: ComponentDefinition
persistent identity: BBa C0052
display id: BBa_C0052
version: 1
was generated by: iGEM to SBOL conversion
description: cI repressor from phage 434 (+LVA)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: Bacteriophage 434
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: The 434 cI repressor protein coding sequence is a 710 base-pair sequence with the standard RBS-compatible BioBrick prefix and the standard BioBrick suffix sections on its ends. It binds to the 434 regulatory sequence, BBa_R0052. The sequence contains a LVA tag for faster degredation and has no RBS.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: References (unparsed) here: Nikolnikov,S., Posfai,G. and Sain,B. The construction of a versatile plasmid vector that allows direct selection of fragments cloned into six unique sites of the cI gene of coliphage 434. Gene 30 (1-3), 261-265 (1984) References (unparsed) here: Nikolnikov,S., Posfai,G. and Sain,B. The construction of a versatile plasmid vector that allows direct selection of fragments cloned into six unique sites of the cI gene of coliphage 434. Gene 30 (1-3), 261-265 (1984)
star: true
creator: Maia Mahoney
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: BBa_C0052
sequence annotation: cI 434
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0052_sequence/1

BBa_C0052
type: SequenceAnnotation
persistent identity: annotation7035
display id: annotation7035
version: 1
top level: cI 434
location: https://synbiohub.org/public/igem/BBa_C0052/annotation7035/range7035/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_C0052/annotation7035/range7035/1
type: Range
persistent identity: range7035
display id: range7035
version: 1
top level: cI 434
direction: direction/forward
start: 1
end: 669
orientation: inline

cI 434
type: SequenceAnnotation
persistent identity: annotation1743
display id: annotation1743
version: 1
top level: cI 434
location: https://synbiohub.org/public/igem/BBa_C0052/annotation1743/range1743/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_C0052/annotation1743/range1743/1
type: Range
persistent identity: range1743
display id: range1743
version: 1
top level: cI 434
direction: direction/forward
start: 1
end: 669
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation1745
display id: annotation1745
version: 1
top level: cI 434
location: https://synbiohub.org/public/igem/BBa_C0052/annotation1745/range1745/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_C0052/annotation1745/range1745/1
type: Range
persistent identity: range1745
display id: range1745
version: 1
top level: cI 434
direction: direction/forward
start: 631
end: 669
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213992
display id: annotation2213992
version: 1
top level: cI 434
location: https://synbiohub.org/public/igem/BBa_C0052/annotation2213992/range2213992/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_C0052/annotation2213992/range2213992/1
type: Range
persistent identity: range2213992
display id: range2213992
version: 1
top level: cI 434
direction: direction/forward
start: 670
end: 694
orientation: inline

BBa_B1006
type: ComponentDefinition
persistent identity: BBa B1006
display id: BBa_B1006
version: 1
was generated by: iGEM to SBOL conversion
description: Terminator (artificial, large, %T~>90)
created: 2006-08-30T11:00:00Z
modified: 2015-08-31T04:07:21Z
mutable provenance: modified E. coli thr terminator, replaced all A-T pairs in stem with C-G pairs
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Artificial terminator, estimated %T~>90% *8bp stem, 6nt loop *Bidirectional, estimated reverse %T~>90%
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_
m user id: 0
owner id: 745
owning group id: 41
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Bidirectional, with the reverse estimated to be less effective than the forward. Has a polyA tail of 9 residues.
star: true
creator: Haiyao Huang
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: B1006
sequence annotation: PolyA
sequence annotation: modified thr terminator
sequence: https://synbiohub.org/public/igem/BBa_B1006_sequence/1

B1006
type: SequenceAnnotation
persistent identity: annotation1898428
display id: annotation1898428
version: 1
top level: BBa_B1006
location: https://synbiohub.org/public/igem/BBa_B1006/annotation1898428/range1898428/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B1006/annotation1898428/range1898428/1
type: Range
persistent identity: range1898428
display id: range1898428
version: 1
top level: BBa_B1006
direction: direction/forward
start: 1
end: 39
orientation: inline

PolyA
type: SequenceAnnotation
persistent identity: annotation1898431
display id: annotation1898431
version: 1
top level: BBa_B1006
location: https://synbiohub.org/public/igem/BBa_B1006/annotation1898431/range1898431/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B1006/annotation1898431/range1898431/1
type: Range
persistent identity: range1898431
display id: range1898431
version: 1
top level: BBa_B1006
direction: direction/reverse
start: 1
end: 9
orientation: inline

modified thr terminator
type: SequenceAnnotation
persistent identity: annotation1898429
display id: annotation1898429
version: 1
top level: BBa_B1006
location: https://synbiohub.org/public/igem/BBa_B1006/annotation1898429/range1898429/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B1006/annotation1898429/range1898429/1
type: Range
persistent identity: range1898429
display id: range1898429
version: 1
top level: BBa_B1006
direction: direction/forward
start: 10
end: 31
orientation: inline

PolyA
type: SequenceAnnotation
persistent identity: annotation1898430
display id: annotation1898430
version: 1
top level: BBa_B1006
location: https://synbiohub.org/public/igem/BBa_B1006/annotation1898430/range1898430/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B1006/annotation1898430/range1898430/1
type: Range
persistent identity: range1898430
display id: range1898430
version: 1
top level: BBa_B1006
direction: direction/forward
start: 32
end: 39
orientation: inline

BBa_I746916
type: ComponentDefinition
persistent identity: BBa I746916
display id: BBa_I746916
version: 1
was generated by: iGEM to SBOL conversion
description: superfolder GFP coding sequence
created: 2008-09-29T11:00:00Z
modified: 2015-08-31T04:08:05Z
mutable provenance: Superfolder GFP was originally described by: Pedelacq et al (2006): "Engineering and characterization of a superfolder green fluorescent protein", Nature Biotech 24 (1) January 2006 This version was synthesised de novo (by Geneart).
owned by: james
owned by: myers
mutable description: This is the coding sequence of superfolder GFP (Pedelacq et al (2006): "Engineering and characterization of a superfolder green fluorescent protein", Nature Biotech 24 (1) January 2006). It carries the following amino acid changes with respect to mut3 GFP (E0040), the currently most commonly used GFP in the registry: S30R, Y39N, F64L, G65T, F99S, N105T, Y145F, M153T, V163A, I171V, A206V Its in-vivo properties are considerably improved with respect to mut3 - it develops fluorescence about 3fold faster than mut3 GFP and reaches 4fold higher absolute fluorescence levels. Fluorescenct colonies can be identified with the naked eye even without UV or blue light illumination (that is to say the amount of blue light in normal daylight or lablight is sufficient). Additionally it is more stable in vitro and refolds faster after in vitro denaturation with respect to mut3 GFP. Note: Superfolder GFP is available in constructs driven by the pBAD and T7 promoters: part numbers I746908 and I746909 respectively. Additionally 6-his tagged versions for protein purification exist: I746914 (pBAD driven) and I746915 (T7 driven).
discontinued: false
dominant: false
group u list: _116_
m user id: 0
owner id: 2122
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Codon optimisation before de novo synthesis was carried out for both, E.coli and Bacillus subtilis.
star: false
creator: Stefan Milde
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: superfolder GFP coding region
sequence annotation: start
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_I746916_sequence/1

superfolder GFP coding region
type: SequenceAnnotation
persistent identity: annotation1977534
display id: annotation1977534
version: 1
top level: BBa_I746916
location: https://synbiohub.org/public/igem/BBa_I746916/annotation1977534/range1977534/1
role: feature/protein
role: SO:0000316

https://synbiohub.org/public/igem/BBa_I746916/annotation1977534/range1977534/1
type: Range
persistent identity: range1977534
display id: range1977534
version: 1
top level: BBa_I746916
direction: direction/forward
start: 1
end: 720
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1977533
display id: annotation1977533
version: 1
top level: BBa_I746916
location: https://synbiohub.org/public/igem/BBa_I746916/annotation1977533/range1977533/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_I746916/annotation1977533/range1977533/1
type: Range
persistent identity: range1977533
display id: range1977533
version: 1
top level: BBa_I746916
direction: direction/forward
start: 1
end: 3
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1977535
display id: annotation1977535
version: 1
top level: BBa_I746916
location: https://synbiohub.org/public/igem/BBa_I746916/annotation1977535/range1977535/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_I746916/annotation1977535/range1977535/1
type: Range
persistent identity: range1977535
display id: range1977535
version: 1
top level: BBa_I746916
direction: direction/forward
start: 715
end: 720
orientation: inline

RhI
type: ComponentDefinition
persistent identity: BBa I14017
display id: BBa_I14017
version: 1
was generated by: iGEM to SBOL conversion
description: P(Rhl)
created: 2004-08-01T11:00:00Z
modified: 2015-08-31T04:07:37Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RhlR and C4HSL regulated promoter.
discontinued: false
dominant: true
experience: experience/None
group u list: _4_
m user id: 0
owner id: 171
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Vikram Vijayan, Allen Hsu, Lawrence Fomundam
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: modified P(Rhl)
sequence annotation: RhlR
sequence annotation: -35
sequence: https://synbiohub.org/public/igem/BBa_I14017_sequence/1

modified P(Rhl)
type: SequenceAnnotation
persistent identity: annotation1043006
display id: annotation1043006
version: 1
top level: RhI
location: https://synbiohub.org/public/igem/BBa_I14017/annotation1043006/range1043006/1
role: feature/operator
role: SO:0000057

https://synbiohub.org/public/igem/BBa_I14017/annotation1043006/range1043006/1
type: Range
persistent identity: range1043006
display id: range1043006
version: 1
top level: RhI
direction: direction/forward
start: 1
end: 51
orientation: inline

RhlR
type: SequenceAnnotation
persistent identity: annotation1043005
display id: annotation1043005
version: 1
top level: RhI
location: https://synbiohub.org/public/igem/BBa_I14017/annotation1043005/range1043005/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_I14017/annotation1043005/range1043005/1
type: Range
persistent identity: range1043005
display id: range1043005
version: 1
top level: RhI
direction: direction/forward
start: 1
end: 20
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1043007
display id: annotation1043007
version: 1
top level: RhI
location: https://synbiohub.org/public/igem/BBa_I14017/annotation1043007/range1043007/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_I14017/annotation1043007/range1043007/1
type: Range
persistent identity: range1043007
display id: range1043007
version: 1
top level: RhI
direction: direction/forward
start: 21
end: 50
orientation: inline

Prm +
type: ComponentDefinition
persistent identity: BBa I12006
display id: BBa_I12006
version: 1
was generated by: iGEM to SBOL conversion
description: Modified lamdba Prm promoter (repressed by 434 cI)
created: 2004-07-13T11:00:00Z
modified: 2015-08-31T04:07:31Z
mutable provenance: Bushman(1993), Shih & Gussin (1983)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Lamdba Prm promoter modified to be activated by lamda repressor (cI) and repressed by 434 repressor (cI)
discontinued: false
dominant: false
experience: experience/None
group u list: _3_
m user id: 0
owner id: 147
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: The O-R1 region of 434 contained 14 base pairs as opposed to the 17 base pairs of the O-R3 site of lambda. Also, it was noticed that the O-R3 site of the lambda included part of the -10 site. Hence, to preserve the spacing and the -10 site, the three nucleotides that were in both the -10 site and the lambda O-R3 site were retained. The 14 nucleotides that were in the O-R3 site and not in the -10 site were replaced with the O-R1 site of the 434.
star: true
creator: mcnamara
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: OR1 lambda
sequence annotation: OR2 lambda
sequence annotation: -35
sequence annotation: OR1 434
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_I12006_sequence/1

OR1 lambda
type: SequenceAnnotation
persistent identity: annotation786500
display id: annotation786500
version: 1
top level: Prm +
location: https://synbiohub.org/public/igem/BBa_I12006/annotation786500/range786500/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_I12006/annotation786500/range786500/1
type: Range
persistent identity: range786500
display id: range786500
version: 1
top level: Prm +
direction: direction/forward
start: 9
end: 25
orientation: inline

OR2 lambda
type: SequenceAnnotation
persistent identity: annotation786518
display id: annotation786518
version: 1
top level: Prm +
location: https://synbiohub.org/public/igem/BBa_I12006/annotation786518/range786518/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_I12006/annotation786518/range786518/1
type: Range
persistent identity: range786518
display id: range786518
version: 1
top level: Prm +
direction: direction/forward
start: 33
end: 49
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation837284
display id: annotation837284
version: 1
top level: Prm +
location: https://synbiohub.org/public/igem/BBa_I12006/annotation837284/range837284/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_I12006/annotation837284/range837284/1
type: Range
persistent identity: range837284
display id: range837284
version: 1
top level: Prm +
direction: direction/forward
start: 48
end: 53
orientation: inline

OR1 434
type: SequenceAnnotation
persistent identity: annotation786365
display id: annotation786365
version: 1
top level: Prm +
location: https://synbiohub.org/public/igem/BBa_I12006/annotation786365/range786365/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_I12006/annotation786365/range786365/1
type: Range
persistent identity: range786365
display id: range786365
version: 1
top level: Prm +
direction: direction/forward
start: 56
end: 69
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation837228
display id: annotation837228
version: 1
top level: Prm +
location: https://synbiohub.org/public/igem/BBa_I12006/annotation837228/range837228/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_I12006/annotation837228/range837228/1
type: Range
persistent identity: range837228
display id: range837228
version: 1
top level: Prm +
direction: direction/forward
start: 71
end: 76
orientation: inline

https://synbiohub.org/public/igem/BBa_K1895995_sequence/1
type: Sequence
persistent identity: BBa K1895995 sequence
display id: BBa_K1895995_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgaggaatgacggaggctttttgctgtggtgggacggtttgcgtagcgagatgcagccgatccacgacagccagggcgtgttcgccgtcctggaaaaggaagtgcggcgcctgggcttcgattactacgcctatggcgtgcgccacacgattcccttcacccggccgaagaccgaggtccatggcacctatcccaaggcctggctggagcgataccagatgcagaactacggggccgtggatccggcgatcctcaacggcctgcgctcctcggaaatggtggtctggagcgacagcctgttcgaccagagccggatgctctggaacgaggctcgcgattggggcctctgtgtcggcgcgaccttgccgatccgcgcgccgaacaatttgctcagcgtgctttccgtggcgcgcgaccagcagaacatctccagcttcgagcgcgaggaaatccgcctgcggctgcgttgcatgatcgagttgctgacccagaagctgaccgacctggagcatccgatgctgatgtccaacccggtctgcctgagccatcgcgaacgcgagatcctgcaatggaccgccgacggcaagagttccggggaaatcgccatcatcctgagcatctccgagagcacggtgaacttccaccacaagaacatccagaagaagttcgacgcgccgaacaagacgctggctgccgcctacgccgcggcgctgggtctcatctaataatactagagaaagaggagaaatactagatgagtatttcttccagggtaaaaagcaaaagaatccagcttggacttaaccaggctgaacttgctcaaaaggtggggactacccagcagtctatagagcagctcgaaaacggtaaaactaagcgaccacgctttttaccagaacttgcgtcagctcttggcgtaagtgttgactggctgctcaatggcacctctgattcgaatgttagatttgttgggcacgttgagcccaaagggaaatatccattgattagcatggttagagctggttcgtggtgtgaagcttgtgaaccctacgatatcaaggacattgatgaatggtatgacagtgacgttaacttattaggcaatggattctggctgaaggttgaaggtgattccatgacctcacctgtaggtcaaagcatccctgaaggtcatatggtgttagtagatactggacgggagccagtgaatggaagccttgttgtagccaaactgactgacgcgaacgaagcaacattcaagaaactggtcatagatggcggtcagaagtacctgaaaggcctgaatccttcatggcctatgactcctatcaacggaaactgcaagattatcggtgttgtcgtggaagcgagggtaaaattcgtagctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagaaagaggagaaatactagatgagtatttcttccagggtaaaaagcaaaagaatccagcttggacttaaccaggctgaacttgctcaaaaggtggggactacccagcagtctatagagcagctcgaaaacggtaaaactaagcgaccacgctttttaccagaacttgcgtcagctcttggcgtaagtgttgactggctgctcaatggcacctctgattcgaatgttagatttgttgggcacgttgagcccaaagggaaatatccattgattagcatggttagagctggttcgtggtgtgaagcttgtgaaccctacga…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0010_sequence/1
type: Sequence
persistent identity: BBa R0010 sequence
display id: BBa_R0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0051_sequence/1
type: Sequence
persistent identity: BBa C0051 sequence
display id: BBa_C0051_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0171_sequence/1
type: Sequence
persistent identity: BBa C0171 sequence
display id: BBa_C0171_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaggaatgacggaggctttttgctgtggtgggacggtttgcgtagcgagatgcagccgatccacgacagccagggcgtgttcgccgtcctggaaaaggaagtgcggcgcctgggcttcgattactacgcctatggcgtgcgccacacgattcccttcacccggccgaagaccgaggtccatggcacctatcccaaggcctggctggagcgataccagatgcagaactacggggccgtggatccggcgatcctcaacggcctgcgctcctcggaaatggtggtctggagcgacagcctgttcgaccagagccggatgctctggaacgaggctcgcgattggggcctctgtgtcggcgcgaccttgccgatccgcgcgccgaacaatttgctcagcgtgctttccgtggcgcgcgaccagcagaacatctccagcttcgagcgcgaggaaatccgcctgcggctgcgttgcatgatcgagttgctgacccagaagctgaccgacctggagcatccgatgctgatgtccaacccggtctgcctgagccatcgcgaacgcgagatcctgcaatggaccgccgacggcaagagttccggggaaatcgccatcatcctgagcatctccgagagcacggtgaacttccaccacaagaacatccagaagaagttcgacgcgccgaacaagacgctggctgccgcctacgccgcggcgctgggtctcatctaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0052_sequence/1
type: Sequence
persistent identity: BBa C0052 sequence
display id: BBa_C0052_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgagtatttcttccagggtaaaaagcaaaagaatccagcttggacttaaccaggctgaacttgctcaaaaggtggggactacccagcagtctatagagcagctcgaaaacggtaaaactaagcgaccacgctttttaccagaacttgcgtcagctcttggcgtaagtgttgactggctgctcaatggcacctctgattcgaatgttagatttgttgggcacgttgagcccaaagggaaatatccattgattagcatggttagagctggttcgtggtgtgaagcttgtgaaccctacgatatcaaggacattgatgaatggtatgacagtgacgttaacttattaggcaatggattctggctgaaggttgaaggtgattccatgacctcacctgtaggtcaaagcatccctgaaggtcatatggtgttagtagatactggacgggagccagtgaatggaagccttgttgtagccaaactgactgacgcgaacgaagcaacattcaagaaactggtcatagatggcggtcagaagtacctgaaaggcctgaatccttcatggcctatgactcctatcaacggaaactgcaagattatcggtgttgtcgtggaagcgagggtaaaattcgtagctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B1006_sequence/1
type: Sequence
persistent identity: BBa B1006 sequence
display id: BBa_B1006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaaaaaaaaccccgcccctgacagggcggggtttttttt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I746916_sequence/1
type: Sequence
persistent identity: BBa I746916 sequence
display id: BBa_I746916_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcgtaaaggcgaagagctgttcactggtgtcgtccctattctggtggaactggatggtgatgtcaacggtcataagttttccgtgcgtggcgagggtgaaggtgacgcaactaatggtaaactgacgctgaagttcatctgtactactggtaaactgccggtaccttggccgactctggtaacgacgctgacttatggtgttcagtgctttgctcgttatccggaccatatgaagcagcatgacttcttcaagtccgccatgccggaaggctatgtgcaggaacgcacgatttcctttaaggatgacggcacgtacaaaacgcgtgcggaagtgaaatttgaaggcgataccctggtaaaccgcattgagctgaaaggcattgactttaaagaagacggcaatatcctgggccataagctggaatacaattttaacagccacaatgtttacatcaccgccgataaacaaaaaaatggcattaaagcgaattttaaaattcgccacaacgtggaggatggcagcgtgcagctggctgatcactaccagcaaaacactccaatcggtgatggtcctgttctgctgccagacaatcactatctgagcacgcaaagcgttctgtctaaagatccgaacgagaaacgcgatcatatggttctgctggagttcgtaaccgcagcgggcatcacgcatggtatggatgaactgtacaaatgatga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I14017_sequence/1
type: Sequence
persistent identity: BBa I14017 sequence
display id: BBa_I14017_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcctgcatgaattggtaggcttacgcaagaaaatggtttgttatagtcgaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I12006_sequence/1
type: Sequence
persistent identity: BBa I12006 sequence
display id: BBa_I12006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcaaccattatcaccgccagaggtaaaatagtcaacacgcacggtgttagatattacaaactttcttgtatagatttaacgt
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z