BBa_K1895996
type: ComponentDefinition
persistent identity: BBa K1895996
display id: BBa_K1895996
version: 1
was generated by: iGEM to SBOL conversion
description: Red light sensitive 'LDR'
created: 2016-07-21T11:00:00Z
modified: 2016-07-22T06:23:40Z
mutable provenance: Hypothetical part from 2016 Newcastle iGEM project.
owned by: james
owned by: myers
mutable description: [TODO]
discontinued: false
dominant: false
group u list: _2361_
m user id: 29859
owner id: 29859
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: [TODO]
star: false
creator: Jake Burton
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0034
component: BBa_B1006
component: BBa_J23100
component: BBa_I15008
component: BBa_I15009
component: BBa_I15010
component: BBa_R0084
component: BBa_K1895998
sequence annotation: BBa_J23100
sequence annotation: BBa_B0034
sequence annotation: BBa_I15008
sequence annotation: BBa_I15009
sequence annotation: BBa_I15010
sequence annotation: BBa_B1006
sequence annotation: BBa_R0084
sequence annotation: BBa_K1895998
sequence: https://synbiohub.org/public/igem/BBa_K1895996_sequence/1

BBa_B0034
type: Component
persistent identity: component2481186
display id: component2481186
version: 1
top level: BBa_K1895996
definition: BBa_B0034
access: public

BBa_B1006
type: Component
persistent identity: component2481192
display id: component2481192
version: 1
top level: BBa_K1895996
definition: BBa_B1006
access: public

BBa_J23100
type: Component
persistent identity: component2481159
display id: component2481159
version: 1
top level: BBa_K1895996
definition: BBa_J23100
access: public

BBa_I15008
type: Component
persistent identity: component2481163
display id: component2481163
version: 1
top level: BBa_K1895996
definition: BBa_I15008
access: public

BBa_B0034
type: Component
persistent identity: component2481161
display id: component2481161
version: 1
top level: BBa_K1895996
definition: BBa_B0034
access: public

BBa_I15009
type: Component
persistent identity: component2481168
display id: component2481168
version: 1
top level: BBa_K1895996
definition: BBa_I15009
access: public

BBa_B0034
type: Component
persistent identity: component2481165
display id: component2481165
version: 1
top level: BBa_K1895996
definition: BBa_B0034
access: public

BBa_I15010
type: Component
persistent identity: component2481173
display id: component2481173
version: 1
top level: BBa_K1895996
definition: BBa_I15010
access: public

BBa_B0034
type: Component
persistent identity: component2481170
display id: component2481170
version: 1
top level: BBa_K1895996
definition: BBa_B0034
access: public

BBa_R0084
type: Component
persistent identity: component2481184
display id: component2481184
version: 1
top level: BBa_K1895996
definition: OmpR
access: public

BBa_B1006
type: Component
persistent identity: component2481178
display id: component2481178
version: 1
top level: BBa_K1895996
definition: BBa_B1006
access: public

BBa_K1895998
type: Component
persistent identity: component2481187
display id: component2481187
version: 1
top level: BBa_K1895996
definition: BBa_K1895998
access: public

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2481159
display id: annotation2481159
version: 1
top level: BBa_K1895996
location: https://synbiohub.org/public/igem/BBa_K1895996/annotation2481159/range2481159/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K1895996/annotation2481159/range2481159/1
type: Range
persistent identity: range2481159
display id: range2481159
version: 1
top level: BBa_K1895996
direction: direction/reverse
start: 1
end: 35
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2481161
display id: annotation2481161
version: 1
top level: BBa_K1895996
location: https://synbiohub.org/public/igem/BBa_K1895996/annotation2481161/range2481161/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1895996/annotation2481161/range2481161/1
type: Range
persistent identity: range2481161
display id: range2481161
version: 1
top level: BBa_K1895996
direction: direction/reverse
start: 44
end: 55
orientation: inline

BBa_I15008
type: SequenceAnnotation
persistent identity: annotation2481163
display id: annotation2481163
version: 1
top level: BBa_K1895996
location: https://synbiohub.org/public/igem/BBa_K1895996/annotation2481163/range2481163/1
component: BBa_I15008

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type: Range
persistent identity: range2481163
display id: range2481163
version: 1
top level: BBa_K1895996
direction: direction/reverse
start: 62
end: 812
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2481165
display id: annotation2481165
version: 1
top level: BBa_K1895996
location: https://synbiohub.org/public/igem/BBa_K1895996/annotation2481165/range2481165/1
component: BBa_B0034

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type: Range
persistent identity: range2481165
display id: range2481165
version: 1
top level: BBa_K1895996
direction: direction/reverse
start: 821
end: 832
orientation: inline

BBa_I15009
type: SequenceAnnotation
persistent identity: annotation2481168
display id: annotation2481168
version: 1
top level: BBa_K1895996
location: https://synbiohub.org/public/igem/BBa_K1895996/annotation2481168/range2481168/1
component: BBa_I15009

https://synbiohub.org/public/igem/BBa_K1895996/annotation2481168/range2481168/1
type: Range
persistent identity: range2481168
display id: range2481168
version: 1
top level: BBa_K1895996
direction: direction/reverse
start: 839
end: 1613
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2481170
display id: annotation2481170
version: 1
top level: BBa_K1895996
location: https://synbiohub.org/public/igem/BBa_K1895996/annotation2481170/range2481170/1
component: BBa_B0034

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type: Range
persistent identity: range2481170
display id: range2481170
version: 1
top level: BBa_K1895996
direction: direction/reverse
start: 1622
end: 1633
orientation: inline

BBa_I15010
type: SequenceAnnotation
persistent identity: annotation2481173
display id: annotation2481173
version: 1
top level: BBa_K1895996
location: https://synbiohub.org/public/igem/BBa_K1895996/annotation2481173/range2481173/1
component: BBa_I15010

https://synbiohub.org/public/igem/BBa_K1895996/annotation2481173/range2481173/1
type: Range
persistent identity: range2481173
display id: range2481173
version: 1
top level: BBa_K1895996
direction: direction/reverse
start: 1640
end: 3902
orientation: inline

BBa_B1006
type: SequenceAnnotation
persistent identity: annotation2481178
display id: annotation2481178
version: 1
top level: BBa_K1895996
location: https://synbiohub.org/public/igem/BBa_K1895996/annotation2481178/range2481178/1
component: BBa_B1006

https://synbiohub.org/public/igem/BBa_K1895996/annotation2481178/range2481178/1
type: Range
persistent identity: range2481178
display id: range2481178
version: 1
top level: BBa_K1895996
direction: direction/reverse
start: 3911
end: 3949
orientation: inline

BBa_R0084
type: SequenceAnnotation
persistent identity: annotation2481184
display id: annotation2481184
version: 1
top level: BBa_K1895996
location: https://synbiohub.org/public/igem/BBa_K1895996/annotation2481184/range2481184/1
component: BBa_R0084

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type: Range
persistent identity: range2481184
display id: range2481184
version: 1
top level: BBa_K1895996
direction: direction/reverse
start: 3958
end: 4065
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2481186
display id: annotation2481186
version: 1
top level: BBa_K1895996
location: https://synbiohub.org/public/igem/BBa_K1895996/annotation2481186/range2481186/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1895996/annotation2481186/range2481186/1
type: Range
persistent identity: range2481186
display id: range2481186
version: 1
top level: BBa_K1895996
direction: direction/reverse
start: 4074
end: 4085
orientation: inline

BBa_K1895998
type: SequenceAnnotation
persistent identity: annotation2481187
display id: annotation2481187
version: 1
top level: BBa_K1895996
location: https://synbiohub.org/public/igem/BBa_K1895996/annotation2481187/range2481187/1
component: BBa_K1895998

https://synbiohub.org/public/igem/BBa_K1895996/annotation2481187/range2481187/1
type: Range
persistent identity: range2481187
display id: range2481187
version: 1
top level: BBa_K1895996
direction: direction/reverse
start: 4092
end: 4295
orientation: inline

BBa_B1006
type: SequenceAnnotation
persistent identity: annotation2481192
display id: annotation2481192
version: 1
top level: BBa_K1895996
location: https://synbiohub.org/public/igem/BBa_K1895996/annotation2481192/range2481192/1
component: BBa_B1006

https://synbiohub.org/public/igem/BBa_K1895996/annotation2481192/range2481192/1
type: Range
persistent identity: range2481192
display id: range2481192
version: 1
top level: BBa_K1895996
direction: direction/reverse
start: 4304
end: 4342
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

OmpR
type: ComponentDefinition
persistent identity: BBa R0084
display id: BBa_R0084
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (OmpR, positive)
created: 2004-01-27T12:00:00Z
modified: 2015-05-08T01:14:15Z
mutable provenance: NC_000913 E. coli K12
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Positively regulated, OmpR-controlled promoter. This promoter is taken from the upstream region of ompF. Phosphorylated OmpR binds to the three operator sites and activates transcription.
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: The promoter includes three OmpR binding sites: F1, F2, and F3. The promoter starts at -107 and goes up to the transcriptional start site at +1. A distant fourth binding site, F4, has been deleted. It is involved in negative regulation of ompF (Head, Tardy and Kenney, 1998), so its deletion is intended to make the promoter solely activating. The efficacy of this promoter versus the ompC upstream region (BBa_R0082) or the truncated ompC upstream region (BBa_R0083) is currently unknown.
star: true
creator: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: F1 OmpR
sequence annotation: F2 OmpR
sequence annotation: F3 OmpR
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0084_sequence/1

F1 OmpR
type: SequenceAnnotation
persistent identity: annotation301308
display id: annotation301308
version: 1
top level: OmpR
location: https://synbiohub.org/public/igem/BBa_R0084/annotation301308/range301308/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0084/annotation301308/range301308/1
type: Range
persistent identity: range301308
display id: range301308
version: 1
top level: OmpR
direction: direction/forward
start: 11
end: 28
orientation: inline

F2 OmpR
type: SequenceAnnotation
persistent identity: annotation301309
display id: annotation301309
version: 1
top level: OmpR
location: https://synbiohub.org/public/igem/BBa_R0084/annotation301309/range301309/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0084/annotation301309/range301309/1
type: Range
persistent identity: range301309
display id: range301309
version: 1
top level: OmpR
direction: direction/forward
start: 31
end: 48
orientation: inline

F3 OmpR
type: SequenceAnnotation
persistent identity: annotation301313
display id: annotation301313
version: 1
top level: OmpR
location: https://synbiohub.org/public/igem/BBa_R0084/annotation301313/range301313/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0084/annotation301313/range301313/1
type: Range
persistent identity: range301313
display id: range301313
version: 1
top level: OmpR
direction: direction/forward
start: 51
end: 68
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation301317
display id: annotation301317
version: 1
top level: OmpR
location: https://synbiohub.org/public/igem/BBa_R0084/annotation301317/range301317/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0084/annotation301317/range301317/1
type: Range
persistent identity: range301317
display id: range301317
version: 1
top level: OmpR
direction: direction/forward
start: 73
end: 78
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation301318
display id: annotation301318
version: 1
top level: OmpR
location: https://synbiohub.org/public/igem/BBa_R0084/annotation301318/range301318/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0084/annotation301318/range301318/1
type: Range
persistent identity: range301318
display id: range301318
version: 1
top level: OmpR
direction: direction/forward
start: 88
end: 93
orientation: inline

BBa_I15008
type: ComponentDefinition
persistent identity: BBa I15008
display id: BBa_I15008
version: 1
was generated by: iGEM to SBOL conversion
description: heme oxygenase (ho1) from Synechocystis
created: 2004-09-19T11:00:00Z
modified: 2015-08-31T04:07:38Z
mutable provenance: Voigt Lab via Anselm Levskaya
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: One of two requisite genes required for the biosynthesis of phycocyanobilin from heme.
discontinued: false
dominant: false
experience: experience/None
group u list: _5_
m user id: 0
owner id: 88
owning group id: 7
sample status: In stock
status: status/Available
bookmark: true
star: true
creator: Jeff Tabor
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_I15008_sequence/1

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214026
display id: annotation2214026
version: 1
top level: BBa_I15008
location: https://synbiohub.org/public/igem/BBa_I15008/annotation2214026/range2214026/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_I15008/annotation2214026/range2214026/1
type: Range
persistent identity: range2214026
display id: range2214026
version: 1
top level: BBa_I15008
direction: direction/forward
start: 727
end: 751
orientation: inline

BBa_J23100
type: ComponentDefinition
persistent identity: BBa J23100
display id: BBa_J23100
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-03T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: Isolated from library of promoters
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Replace later
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23100_sequence/1

BBa_I15009
type: ComponentDefinition
persistent identity: BBa I15009
display id: BBa_I15009
version: 1
was generated by: iGEM to SBOL conversion
description: phycocyanobilin:ferredoxin oxidoreductase (PcyA) from synechocystis
created: 2004-09-19T11:00:00Z
modified: 2015-08-31T04:07:38Z
mutable provenance: Voigt Lab via Anselm Levskaya
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Second of two required phycocyanobilin biosynthetic genes.
discontinued: false
dominant: false
experience: experience/None
group u list: _5_
m user id: 0
owner id: 88
owning group id: 7
sample status: In stock
status: status/Available
bookmark: true
star: true
creator: Jeff Tabor
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: PcyA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_I15009_sequence/1

PcyA
type: SequenceAnnotation
persistent identity: annotation1891612
display id: annotation1891612
version: 1
top level: BBa_I15009
location: https://synbiohub.org/public/igem/BBa_I15009/annotation1891612/range1891612/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_I15009/annotation1891612/range1891612/1
type: Range
persistent identity: range1891612
display id: range1891612
version: 1
top level: BBa_I15009
direction: direction/forward
start: 1
end: 750
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214032
display id: annotation2214032
version: 1
top level: BBa_I15009
location: https://synbiohub.org/public/igem/BBa_I15009/annotation2214032/range2214032/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_I15009/annotation2214032/range2214032/1
type: Range
persistent identity: range2214032
display id: range2214032
version: 1
top level: BBa_I15009
direction: direction/forward
start: 751
end: 775
orientation: inline

BBa_I15010
type: ComponentDefinition
persistent identity: BBa I15010
display id: BBa_I15010
version: 1
was generated by: iGEM to SBOL conversion
description: cph8 (Cph1/EnvZ fusion)
created: 2004-09-19T11:00:00Z
modified: 2015-08-31T04:07:38Z
mutable provenance: Voigt Lab via Anselm Levskaya
owned by: james
owned by: myers
mutable description: Chimeric Cph1 light receptor/EnvZ protein.
discontinued: false
dominant: false
experience: experience/None
group u list: _5_
m user id: 0
owner id: 88
owning group id: 7
sample status: In stock
status: status/Available
bookmark: true
mutable notes: Silent mutation at base 108 (G-A) to remove PstI site
star: false
creator: Jeff Tabor
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Cph8
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_I15010_sequence/1

Cph8
type: SequenceAnnotation
persistent identity: annotation1891613
display id: annotation1891613
version: 1
top level: BBa_I15010
location: https://synbiohub.org/public/igem/BBa_I15010/annotation1891613/range1891613/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_I15010/annotation1891613/range1891613/1
type: Range
persistent identity: range1891613
display id: range1891613
version: 1
top level: BBa_I15010
direction: direction/forward
start: 1
end: 2238
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214031
display id: annotation2214031
version: 1
top level: BBa_I15010
location: https://synbiohub.org/public/igem/BBa_I15010/annotation2214031/range2214031/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_I15010/annotation2214031/range2214031/1
type: Range
persistent identity: range2214031
display id: range2214031
version: 1
top level: BBa_I15010
direction: direction/forward
start: 2239
end: 2263
orientation: inline

BBa_B1006
type: ComponentDefinition
persistent identity: BBa B1006
display id: BBa_B1006
version: 1
was generated by: iGEM to SBOL conversion
description: Terminator (artificial, large, %T~>90)
created: 2006-08-30T11:00:00Z
modified: 2015-08-31T04:07:21Z
mutable provenance: modified E. coli thr terminator, replaced all A-T pairs in stem with C-G pairs
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Artificial terminator, estimated %T~>90% *8bp stem, 6nt loop *Bidirectional, estimated reverse %T~>90%
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_
m user id: 0
owner id: 745
owning group id: 41
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Bidirectional, with the reverse estimated to be less effective than the forward. Has a polyA tail of 9 residues.
star: true
creator: Haiyao Huang
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: B1006
sequence annotation: PolyA
sequence annotation: modified thr terminator
sequence: https://synbiohub.org/public/igem/BBa_B1006_sequence/1

B1006
type: SequenceAnnotation
persistent identity: annotation1898428
display id: annotation1898428
version: 1
top level: BBa_B1006
location: https://synbiohub.org/public/igem/BBa_B1006/annotation1898428/range1898428/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B1006/annotation1898428/range1898428/1
type: Range
persistent identity: range1898428
display id: range1898428
version: 1
top level: BBa_B1006
direction: direction/forward
start: 1
end: 39
orientation: inline

PolyA
type: SequenceAnnotation
persistent identity: annotation1898431
display id: annotation1898431
version: 1
top level: BBa_B1006
location: https://synbiohub.org/public/igem/BBa_B1006/annotation1898431/range1898431/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B1006/annotation1898431/range1898431/1
type: Range
persistent identity: range1898431
display id: range1898431
version: 1
top level: BBa_B1006
direction: direction/reverse
start: 1
end: 9
orientation: inline

modified thr terminator
type: SequenceAnnotation
persistent identity: annotation1898429
display id: annotation1898429
version: 1
top level: BBa_B1006
location: https://synbiohub.org/public/igem/BBa_B1006/annotation1898429/range1898429/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B1006/annotation1898429/range1898429/1
type: Range
persistent identity: range1898429
display id: range1898429
version: 1
top level: BBa_B1006
direction: direction/forward
start: 10
end: 31
orientation: inline

PolyA
type: SequenceAnnotation
persistent identity: annotation1898430
display id: annotation1898430
version: 1
top level: BBa_B1006
location: https://synbiohub.org/public/igem/BBa_B1006/annotation1898430/range1898430/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B1006/annotation1898430/range1898430/1
type: Range
persistent identity: range1898430
display id: range1898430
version: 1
top level: BBa_B1006
direction: direction/forward
start: 32
end: 39
orientation: inline

BBa_K1895998
type: ComponentDefinition
persistent identity: BBa K1895998
display id: BBa_K1895998
version: 1
was generated by: iGEM to SBOL conversion
description: SmtA metallothionein and LVA tag.
created: 2016-07-20T11:00:00Z
modified: 2016-07-21T05:17:31Z
mutable provenance: Variant of BBa_K519010 with protein degradation tag from Anderson et al. (1998)
owned by: james
owned by: myers
mutable description: This is part BBa_K519010, SmtA which is a metallothionein that can bind to heavy metal ions. This variant has an LVA degradation tag before the stop codon which reduces its half-life in the cell.
discontinued: false
dominant: false
group u list: _2361_
m user id: 29859
owner id: 29859
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: [TODO]
star: false
creator: Jake Burton
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_K1895998_sequence/1

https://synbiohub.org/public/igem/BBa_K1895996_sequence/1
type: Sequence
persistent identity: BBa K1895996 sequence
display id: BBa_K1895996_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacggctagctcagtcctaggtacagtgctagctactagagaaagaggagaaatactagatgagtgtcaacttagcttcccagttgcgggaagggacgaaaaaatcccactccatggcggagaacgtcggctttgtcaaatgcttcctcaagggcgttgtcgagaaaaattcctaccgtaagctggttggcaatctctactttgtctacagtgccatggaagaggaaatggcaaaatttaaggaccatcccatcctcagccacatttacttccccgaactcaaccgcaaacaaagcctagagcaagacctgcaattctattacggctccaactggcggcaagaagtgaaaatttctgccgctggccaagcctatgtggaccgagtccggcaagtggccgctacggcccctgaattgttggtggcccattcctacacccgttacctgggggatctttccggcggtcaaattctcaagaaaattgcccaaaatgccatgaatctccacgatggtggcacagctttctatgaatttgccgacattgatgacgaaaaggcttttaaaaatacctaccgtcaagctatgaatgatctgcccattgaccaagccaccgccgaacggattgtggatgaagccaatgacgcctttgccatgaacatgaaaatgttcaacgaacttgaaggcaacctgatcaaggcgatcggcattatggtgttcaacagcctcacccgtcgccgcagtcaaggcagcaccgaagttggcctcgccacctccgaaggctaataacgctgatagtgctagtgtagatcgctactagagaaagaggagaaatactagatggccgtcactgatttaagtttgaccaattcttccctgatgcctacgttgaacccgatgattcaacagttggccctggcgatcgccgctagttggcaaagtttacccctcaagccctatcaattgccggaggatttgggctacgtagaaggccgcctggaaggggaaaagttagtgattgaaaatcggtgctaccaaacgccccagtttcgcaaaatgcatttggagttggccaaggtgggcaaagggttggatattctccactgtgtaatgtttcctgagcctttatacggtctacctttgtttggctgtgacattgtggccggccccggtggagtaagtgcggctattgcggatctatcccccacccaaagcgatcgccaattgcccgcagcgtaccaaaaatcattggcagagctaggccagccagaatttgagcaacaacgggaattgcccccctggggagaaatattttctgaatattgtttattcatccgtcccagcaatgtcactgaagaagaaagatttgtacaaagggtagtggactttttgcaaattcattgtcaccaatccatcgttgccgaacccttgtctgaagctcaaactttggagcaccgtcaggggcaaattcattactgccaacaacaacagaaaaatgataaaacccgtcgggtactggaaaaagcttttggggaagcttgggcggaacggtatatgagccaagtcttatttgatgttatccaataataactctgatagtgctagtgtagatctctactagagaaagaggagaaatactagatggccaccaccgtacaactcagcgaccaatccctccgtcagctagaaaccctcgccatccacaccgcccacctgattcagccccacggtttagtggtggtcctgcaagaaccagacctcaccatcagccaaattagcgccaactgcaccggcattttagggcgatcgccagaggatttgttgggcagaaccctaggggaagtgtttgatagctttcagattgatcccatccagagtcgcctaacggccggacaaatcagcagcctcaaccccagtaaactttgggcgcgggtcatgggggacgactttgtcatttttgacggggtttttcatcgcaacagtgacggtttattggtatgtg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0084_sequence/1
type: Sequence
persistent identity: BBa R0084 sequence
display id: BBa_R0084_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cttaaattttacttttggttacatattttttctttttgaaaccaaatctttatctttgtagcactttcacggtagcgaaacgttagtttgaatggaaagatgcctgca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I15008_sequence/1
type: Sequence
persistent identity: BBa I15008 sequence
display id: BBa_I15008_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgagtgtcaacttagcttcccagttgcgggaagggacgaaaaaatcccactccatggcggagaacgtcggctttgtcaaatgcttcctcaagggcgttgtcgagaaaaattcctaccgtaagctggttggcaatctctactttgtctacagtgccatggaagaggaaatggcaaaatttaaggaccatcccatcctcagccacatttacttccccgaactcaaccgcaaacaaagcctagagcaagacctgcaattctattacggctccaactggcggcaagaagtgaaaatttctgccgctggccaagcctatgtggaccgagtccggcaagtggccgctacggcccctgaattgttggtggcccattcctacacccgttacctgggggatctttccggcggtcaaattctcaagaaaattgcccaaaatgccatgaatctccacgatggtggcacagctttctatgaatttgccgacattgatgacgaaaaggcttttaaaaatacctaccgtcaagctatgaatgatctgcccattgaccaagccaccgccgaacggattgtggatgaagccaatgacgcctttgccatgaacatgaaaatgttcaacgaacttgaaggcaacctgatcaaggcgatcggcattatggtgttcaacagcctcacccgtcgccgcagtcaaggcagcaccgaagttggcctcgccacctccgaaggctaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23100_sequence/1
type: Sequence
persistent identity: BBa J23100 sequence
display id: BBa_J23100_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacggctagctcagtcctaggtacagtgctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1895998_sequence/1
type: Sequence
persistent identity: BBa K1895998 sequence
display id: BBa_K1895998_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgacctcaacaacgttggtcaaatgcgcttgtgagccctgtctctgcaacgtcgatcccagcaaagcgatcgatcgcaacggtctgtactactgcagcgaagcctgtgccgatggccacaccggtggtagcaaaggctgcggccacaccggctgtaactgccacggcgcagcaaacgacgaaaactacgctcttgttgcttaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I15009_sequence/1
type: Sequence
persistent identity: BBa I15009 sequence
display id: BBa_I15009_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggccgtcactgatttaagtttgaccaattcttccctgatgcctacgttgaacccgatgattcaacagttggccctggcgatcgccgctagttggcaaagtttacccctcaagccctatcaattgccggaggatttgggctacgtagaaggccgcctggaaggggaaaagttagtgattgaaaatcggtgctaccaaacgccccagtttcgcaaaatgcatttggagttggccaaggtgggcaaagggttggatattctccactgtgtaatgtttcctgagcctttatacggtctacctttgtttggctgtgacattgtggccggccccggtggagtaagtgcggctattgcggatctatcccccacccaaagcgatcgccaattgcccgcagcgtaccaaaaatcattggcagagctaggccagccagaatttgagcaacaacgggaattgcccccctggggagaaatattttctgaatattgtttattcatccgtcccagcaatgtcactgaagaagaaagatttgtacaaagggtagtggactttttgcaaattcattgtcaccaatccatcgttgccgaacccttgtctgaagctcaaactttggagcaccgtcaggggcaaattcattactgccaacaacaacagaaaaatgataaaacccgtcgggtactggaaaaagcttttggggaagcttgggcggaacggtatatgagccaagtcttatttgatgttatccaataataactctgatagtgctagtgtagatctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I15010_sequence/1
type: Sequence
persistent identity: BBa I15010 sequence
display id: BBa_I15010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggccaccaccgtacaactcagcgaccaatccctccgtcagctagaaaccctcgccatccacaccgcccacctgattcagccccacggtttagtggtggtcctgcaagaaccagacctcaccatcagccaaattagcgccaactgcaccggcattttagggcgatcgccagaggatttgttgggcagaaccctaggggaagtgtttgatagctttcagattgatcccatccagagtcgcctaacggccggacaaatcagcagcctcaaccccagtaaactttgggcgcgggtcatgggggacgactttgtcatttttgacggggtttttcatcgcaacagtgacggtttattggtatgtgaactcgagccagcctacacttccgataatctgcccttcctcggtttttatcacatggccaacgctgccctgaatcggttgcgccaacaagctaatctacgggatttctacgatgttattgtcgaagaagtccgccgtatgactggctttgaccgggtgatgctataccgctttgatgaaaataaccacggtgatgtcattgccgaagataaacgggatgatatggaaccctatttgggcctgcactatcccgaatcggatattccccaacccgcccgtcggctatttatccacaaccccattcgagtaattcccgatgtttatggtgtggcggtgcccctgaccccagcggttaaccccagcaccaaccgagcggtggatttaacagaatccattctgcgcagtgcgtaccattgccacttgacctatctgaaaaatatgggggtaggagcgtctttaaccatttccctaattaaggacggccatctctgggggctcattgcctgccaccatcaaacccccaaagtaattccctttgaactgcgtaaagcctgcgaattttttggtcgggtggtgtttagcaacatttccgcccaggaagatacggaaaccttcgattaccgggtgcagctggcggagcatgaagcggttttattggacaaaatgaccacggcggcggattttgtcgaaggattaactaatcatcccgatcgcctgttgggattaacgggctcccagggggcggccatttgctttggggaaaaattgattttagtaggggaaaccccggacgagaaagcagtgcaatatttactgcaatggttggagaatcgggaagtgcaagacgttttcttcacctcttccctctcacaaatttatcctgatgcagtgaattttaaatccgtggccagtggcttattggccattcccattgcccgtcacaactttttgctctggtttcgccctgaagtgttgcaaacggttaattggggcggtgacccaaatcatgcttacgaagctacccaggaagacggtaaaatcgagctccatccccgccaatcctttgacctctggaaagaaattgtccgactccaatctttgccctggcaatcggtggaaatccaaagtgccctggccctgaaaaaggcgatcgtcaacctcattttgcgccaggcagaagaattgcatatggcggctggtgttaagcaactggcggatgaccgcacgctgctgatggcgggggtaagtcacgacttgcgcacgccgctgacgcgtattcgcctggcgactgagatgatgagcgagcaggatggctatctggcagaatcgatcaataaagatatcgaagagtgcaacgccatcattgagcagtttatcgactacctgcgcaccgggcaggagatgccgatggaaatggcggatcttaatgcagtactcggtgaggtgattgctgccgaaagtggctatgagcgggaaattgaaaccgcgctttaccccggcagcattgaagtgaaaatgcacccgctgtcgatcaaacgcgcggtggcgaatatggtggtcaacgccgcccgttatggcaatggctgggtcaaagtcagcagcggaacggagccgaatcgcgcctggttccaggtggaaga…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B1006_sequence/1
type: Sequence
persistent identity: BBa B1006 sequence
display id: BBa_B1006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaaaaaaaaccccgcccctgacagggcggggtttttttt
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z