BBa_B1006 1 BBa_B1006 Terminator (artificial, large, %T~>90) 2006-08-30T11:00:00Z 2015-08-31T04:07:21Z modified E. coli thr terminator, replaced all A-T pairs in stem with C-G pairs Released HQ 2013 Artificial terminator, estimated %T~>90% *8bp stem, 6nt loop *Bidirectional, estimated reverse %T~>90% false true _41_ 0 745 41 In stock false Bidirectional, with the reverse estimated to be less effective than the forward. Has a polyA tail of 9 residues. true Haiyao Huang annotation1898431 1 PolyA range1898431 1 1 9 annotation1898430 1 PolyA range1898430 1 32 39 annotation1898428 1 B1006 range1898428 1 1 39 annotation1898429 1 modified thr terminator range1898429 1 10 31 BBa_K1895999 1 BBa_K1895999 Arabinose controlled 'resistor' 2016-07-20T11:00:00Z 2016-07-21T04:26:09Z Hypothetical design for the 2016 Newcastle iGEM team. [TODO] false false _2361_ 29859 29859 9 false [TODO] false Jake Burton component2481086 1 BBa_B1006 component2481081 1 BBa_K090000 component2481079 1 BBa_R0080 component2481080 1 BBa_M36405 annotation2481081 1 BBa_K090000 range2481081 1 252 1445 annotation2481080 1 BBa_M36405 range2481080 1 158 245 annotation2481079 1 BBa_R0080 range2481079 1 1 149 annotation2481086 1 BBa_B1006 range2481086 1 1454 1492 BBa_K090000 1 BBa_K090000 Glutamate gated K+ channel 2008-10-22T11:00:00Z 2015-05-08T01:08:37Z From Genomic sequence This part encodes the gene GluR0 from Synechocystis. It is a well characterised glutamate-gated K+ membrane channel, which has a considerable degree of structural and functional homology to rat neurone GluR2 AMPA receptors. false false _188_ 0 3483 9 It's complicated false From peptide sequence of Synechocystis PCC 6803, codon optimised for E.coli, restriction sites removed, and synthesised de novo. false Ellis O'Neill BBa_M36405 1 BBa_M36405 Bicistronic RBS (medium) pFAB1646 2012-12-05T12:00:00Z 2015-05-08T01:14:04Z More information can be found at BIOFAB.org. This is a bicistronic RBS, which consists of a constitutive RBS, and a leader sequence that includes the second RBS. It uses bicistronic junction architecture to tightly control translation. It contains two ribosome binding sites: one controls the gene of interest, and the another synthesizes a short leader protein. The latter bicistronic's translation prevents the former RBS from forming a secondary structure with the gene of interest. This ensures a more consistent expression level of your gene of interest. false false _848_ 0 15032 9 Not in stock false This RBS is of medium strength. false Justo A. Caballero, Shamik Mascharak and Sibel Sayiner BBa_R0080 1 AraC Promoter (AraC regulated) 2004-01-27T12:00:00Z 2015-05-08T01:14:15Z GenBank: J01641 (www.ncbi.nlm.nih.gov) Released HQ 2013 AraC operator, truncated to include araO1, araI1, araI2, c-amp1, and c-amp2 sites. This operator should *activate* transcription in the presence of AraC; b/c the operator lacks the araO2 site, there should not be araC-mediated repression. false false _1_ 0 24 7 In stock false true Sara Neves (Fighting Darwins) annotation301458 1 c-amp1 range301458 1 43 72 annotation308601 1 -35 range308601 1 113 118 annotation308602 1 -10 range308602 1 136 141 annotation301456 1 c-amp2 range301456 1 4 29 annotation301457 1 araO1 range301457 1 6 44 annotation301462 1 ara1 and ara2 range301462 1 73 101 BBa_K1895999_sequence 1 gcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccattactagaggggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgcaggggatattttctgatgtactagatgctgatcctgcgtcgtctgatcctggttgtttctctgtctttcgcaactattgcaattgacatcactacccgtccgggtctggaaccggttcagggtcagggtgtcgaaaccgtagatagccagacgctgaaagttggcgttgttggtaacccgccgttcgttttctatggtgaaggtaaaaacgcggcgttcactggtatctctctggacgtttggcgtgcggtagcagaatcccaaaaatggaactctgagtatgttcgtcagaacagcatctctgctggcatcacggcggtcgcagaaggtgaactggacatcctgattggcccaattagcgtgaccccggaacgcgctgctatcgaaggtattactttcacccagccgtacttttcttctggtattggtctgctgatcccgggcaaaccggtgtccctgtgggaacgtttctctccgttctttggcattgcagctctgtctagcgctggcgttctgactctgctgctgttcctggtaggtaacctgatttggctggcggaacaccgcaaaaacccagaacagttcagcccgcactaccctgaaggcgttcagaacggtatgtggttcgccctggtgaccctgaccaccgtgggctacggcgatcgtagcccgcgtactaagctgggccagctggtagcgggtgtttggatgctggtagcgctgctgtctttttctagcattaccgccggtctggcgagcgctttcagcaccgcactgtctgaagcgtctgctacgccactgttccgctccgtcggcgacctgaaaaacaaggaagtcgctgttgtgcgtgacaccaccgctgtagactgggctaatttctaccaggcggatgtacgtgaaactaacaacctgaccgctgcaatcaccctgctgcaaaagaagcaagtagaggccgtcatgttcgaccgtccggctctgatctattatactcgtcagaaccctaacctgaatctggaggtgactgaaatccgtgtttctctggaaccttacggcttcgttctgaaagaaaactctccgctgcaaaagactattaacgtggaaatgctgaacctgctgtattctcgcgtcattgctgaatttaccgaacgttggctgggtccaggcatcgaagagaaccaggatctgctgccacagaacattggcgaatccccgtcctaatactagagaaaaaaaaaccccgcccctgacagggcggggtttttttt BBa_M36405_sequence 1 gggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgcaggggatattttctgatg BBa_B1006_sequence 1 aaaaaaaaaccccgcccctgacagggcggggtttttttt BBa_K090000_sequence 1 atgctgatcctgcgtcgtctgatcctggttgtttctctgtctttcgcaactattgcaattgacatcactacccgtccgggtctggaaccggttcagggtcagggtgtcgaaaccgtagatagccagacgctgaaagttggcgttgttggtaacccgccgttcgttttctatggtgaaggtaaaaacgcggcgttcactggtatctctctggacgtttggcgtgcggtagcagaatcccaaaaatggaactctgagtatgttcgtcagaacagcatctctgctggcatcacggcggtcgcagaaggtgaactggacatcctgattggcccaattagcgtgaccccggaacgcgctgctatcgaaggtattactttcacccagccgtacttttcttctggtattggtctgctgatcccgggcaaaccggtgtccctgtgggaacgtttctctccgttctttggcattgcagctctgtctagcgctggcgttctgactctgctgctgttcctggtaggtaacctgatttggctggcggaacaccgcaaaaacccagaacagttcagcccgcactaccctgaaggcgttcagaacggtatgtggttcgccctggtgaccctgaccaccgtgggctacggcgatcgtagcccgcgtactaagctgggccagctggtagcgggtgtttggatgctggtagcgctgctgtctttttctagcattaccgccggtctggcgagcgctttcagcaccgcactgtctgaagcgtctgctacgccactgttccgctccgtcggcgacctgaaaaacaaggaagtcgctgttgtgcgtgacaccaccgctgtagactgggctaatttctaccaggcggatgtacgtgaaactaacaacctgaccgctgcaatcaccctgctgcaaaagaagcaagtagaggccgtcatgttcgaccgtccggctctgatctattatactcgtcagaaccctaacctgaatctggaggtgactgaaatccgtgtttctctggaaccttacggcttcgttctgaaagaaaactctccgctgcaaaagactattaacgtggaaatgctgaacctgctgtattctcgcgtcattgctgaatttaccgaacgttggctgggtccaggcatcgaagagaaccaggatctgctgccacagaacattggcgaatccccgtcctaa BBa_R0080_sequence 1 gcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccat igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z