BBa_K1896002
1
BBa_K1896002
inaZ (GG safe)
2016-10-11T11:00:00Z
2016-10-14T05:25:33Z
This part was based on [[Part:BBa_K584021|BBa_K584021]].
This is an improved version of [[Part:BBa_K584021|BBa_K584021]], which encodes the ice nucleating protein gene ''inaZ'' from ''Pseudomonas syringae''. Two BsaI sites have been removed to make the part safe for Golden Gate cloning.
false
false
_2362_
19009
19009
9
false
The many repeats in this gene seem to make this sequence virtually impossible to synthesize, even after changing the codons used. Over-expression of this part at high levels is toxic.
false
Bob Van Hove, Maarten Van Brempt
annotation2495366
1
inaZ
range2495366
1
1
3603
annotation2495367
1
start
range2495367
1
1
3
annotation2495368
1
stop
range2495368
1
3601
3603
annotation2495369
1
repeat region
range2495369
1
526
3453
BBa_K1896002_sequence
1
atgaatctcgacaaggcgttggtgctgcgtacctgtgcaaataacatggccgatcactgcggccttatatggcccgcgtccggcacggtggaatccagatactggcagtcaaccaggcggcatgagaatggtctggtcggtttactgtggggcgctggaaccagcgcttttctaagcgtgcatgccgatgctcgatggattgtctgtgaagttgccgttgcagacatcatcagtctggaagagccgggaatggtcaagtttccgcgggccgaggtggttcatgtcggcgacaggatcagcgcgtcacacttcatttcggcacgtcaggccgaccctgcgtcaacatcaacatcaacgttaacgccaatgcccactgccatacccacgcccatgcctgccgtagcaagtgtcacgttaccggtggccgaacaggcccgtcatgaagtgttcgatgtcgcgtcggtcagcgcggctgccgccccagtaaacaccctgccggtgacgacgccgcagaatgtgcagaccgccacttacggcagcacgttgagtggcgacaatcacagtcgtctgattgccggttatggcagtaacgaaaccgctggcaaccacagtgatctaattgccggttatggaagtacaggcaccgccggctccgacagctggctggtcgctggctatggaagcacccagaccgccggtggggacagcgcgctgacagcgggttacggcagcacccagaccgcccgcgaaggcagcaacctgacggcagggtacggcagcaccggcacggcaggctcggacagttcgctgatcgccggttacggcagtactcagacttcgggcggggacagctcactcacagcgggttacggcagcacgcaaacggctcaggaaggcagcaatctcaccgctgggtatggcagcaccggcacggcaggctcggacagctccttgatcgccggttatggcagtacacaaacctcgggaggcgacagttcgctgaccgcgggctacggcagtacgcagacggcccaggagggcagcaatctgacggcggggtacggcagcacgggtacagcaggtgtcgacagctctctgatcgcgggatacggcagcacgcagacctcgggaagtgacagcgccctgaccgcaggctatggcagcacgcaaacggcccaggaaggcagcaatctcactgctgggtatggcagcaccggcacggcaggttccgacagctcgctgatcgccggttacggcagcacgcaaacctcgggcagtgacagctcgctcacggcggggtacggcagtacgcagacggctcaggaaggcagcattctgacggcggggtacggcagcacgggtacagcaggtgtcgacagttcgttgatcgccggatatggcagcacgcagacctcgggaagtgacagtgcgctgacagcgggttacggcagcacgcaaacggcccaggaaggcagcaacctgacggcgggctacggcagcactggcacggcaggtgccgacagttcgttgatcgccggatatggcagcacgcagacgtcaggcagcgaaagttcgcttaccgcaggctatggcagtacccagactgcccgtgagggcagcaccctgacggccggatatggcagtaccggaacagctggcgctgacagctcgctgatcgccggttacggcagcacgcaaacctcgggcagtgaaagctcgctcacggcaggttatggcagtacccagaccgcacagcagggcagcgtactcacatcaggctatggcagtacgcaaacggccggggctgccagtaacctcaccaccggttacggaagtacaggtaccgcaggtcacgagagtttcatcattgcgggttatggaagtacacagacagcgggccacaaaagtatcctgaccgctggttatggcagtactcagacggccagggacggtagcgacctgattgcgggctatggcagtacgggaaccgcaggctcgggcagttcgctgatcgcaggttatggcagcacccagaccgcgagttacagaagcatgctgaccgccggttatggcagtacccagaccgccagagaacacagcgaccttgtcacaggctatggcagcacttcaacggcagggtcaaacagttcgctgatcgccggctatggaagcactcagacggcgggcttcaaaagcatactgaccgccggttacggcagtacccagacggcacaggagcgcacgagcctggtcgcaggctacggaagcacgtcgactgcgggctattccagttccttgatcgccggctatggcagcacgcagacggcaggctacgaaagcacgttgaccgccggttacggcagtacgcaaaccgctcaggaaaacagctcgctcaccacaggttacggaagtacttctactgcgggctattccagctcgctcatcgcgggttacggcagtacgcaaacggcaggctacgagagcacgttgaccgccggttacggtagtacgcaaaccgcgcaggagcgcagtgatctggtgacaggttatggaagtacctccaccgccggctatgcgagctcgctgattgcgggttatggcagcacgcagactgcgggttatgagagcacgttgaccgccggttacggcagcacgcaaaccgcacaggaaaacagctcgctcaccaccgggtacggaagtacctccacagccggctttgccagctcgctgatctccggttatggcagtacgcagacagccggctataaaagtaccctcacggccggttacggcagtactcagaccgcagagtatggaagctcactcactgcgggctacggcagcactgcaacggccgggcaggacagttcattgatagccggctatggcagctccctgaccagcggaatcagaagttttctgacggcaggctatggcagtacgctgatcgccggacttcgcagcgttttgatcgccggttatggcagtagtcttacatcgggcgttcgcagcacgttgactgcgggttatggcagtaaccagattgcaagttacggcagctcgttgattgcaggccatgaaagcattcaggtcgccggaaataaaagcatgctgatcgccggcaagggcagctcgcagacagcaggttttcgcagcacgctgattgccggtgcgggcagtgtacaactggcgggtgatcgcagccggttgattgccggtgcagacagtaatcagaccgcgggtgaccgcagcaaactgctggccggtaataacagttatctgactgccggcgatagaagcaaactgaccggcgggcatgactgcaccctgatggcgggtgaccaaagcagattgaccgctggtaagaacagtgtcttgacggcaggcgctcgtagcaaactgattggcagtgaaggctcgacgctctcggctggagaagactccatacttattttcaggctctgggacgggaagaggtacaggcaactggtcgccagaacgggtgagaacggtgttgaggccgacataccgtattacgtgaacgaagatgacgatattgtcgataaacccgacgaggacgatgactggatagaggtaaagtag
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z