BBa_K1896004
1
BBa_K1896004
linker-Streptavidin-His (tetrameric)
2016-10-12T11:00:00Z
2016-10-14T05:41:27Z
[[Part:BBa_K283010|Part:BBa_K283010]]
This is an improved version of [[Part:BBa_K283010|Part:BBa_K283010]], which encodes for the core Streptavidin protein of ''Streptomyces avidinii''. The start codon was replaced by a GSTGS linker, a His-tag was added to the C-terminus and one BsaI site was removed.
false
false
_2362_
19009
19009
9
false
One BsaI site was removed to enable Golden Gate cloning.
false
Bob Van Hove, Maarten Van Brempt
annotation2497040
1
GSTGS linker
range2497040
1
1
15
annotation2497045
1
stop
range2497045
1
415
420
annotation2497042
1
Streptavidin
range2497042
1
16
396
annotation2497044
1
His-tag
range2497044
1
397
414
annotation2497041
1
BamHI site removed
range2497041
1
60
60
BBa_K1896004_sequence
1
ggcagcaccggatctgctgaagctggtatcaccggcacctggtacaaccagctgggatctaccttcatcgttaccgctggtgctgacggtgctctgaccggtacctacgaatccgctgttggtaacgctgaaagccgctacgttctgaccggtcgttacgactccgctccggctaccgacggttccggaaccgctctgggttggaccgttgcttggaaaaacaactaccgtaacgctcactccgctaccacctggtctggccagtacgttggtggtgctgaagctcgtatcaacacccagtggttgttgacctccggcaccaccgaagccaacgcgtggaaatccaccctggttggtcacgacaccttcaccaaagttaaaccgtccgctgcttcccatcaccatcaccaccattaataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z